Recombinant
RabMAb

Recombinant Anti-CD39 antibody [EPR20461] (ab223843)

Overview

  • Product name

    Anti-CD39 antibody [EPR20461]
    See all CD39 primary antibodies
  • Description

    Rabbit monoclonal [EPR20461] to CD39
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CD39 aa 50-150. The exact sequence is proprietary.
    Database link: P49961

  • Positive control

    • WB: Human fetal heart, fetal spleen, tonsil and fetal kidney lysates. IHC-P: Human pancreas, colon carcinoma and tonsil tissues.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab223843 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 78 kDa (predicted molecular weight: 58 kDa).
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Could also be implicated in the prevention of platelet aggregation. Hydrolyzes ATP and ADP equally well.
  • Tissue specificity

    Expressed primarily on activated lymphoid cells. Also expressed in endothelial tissues. The vascular isoform and the placental isoform II are present in both placenta and umbilical vein, whereas placental isoform I is present in placenta only.
  • Sequence similarities

    Belongs to the GDA1/CD39 NTPase family.
  • Post-translational
    modifications

    The N-terminus is blocked.
    Palmitoylated in the N-terminal part.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Form

    There are 3 isoforms produced by alternative splicing.
  • Alternative names

    • ATPDase antibody
    • CD 39 antibody
    • CD39 antibody
    • CD39 antigen antibody
    • DKFZp686D194 antibody
    • DKFZp686I093 antibody
    • Ecto apyrase antibody
    • Ecto ATP diphosphohydrolase antibody
    • Ecto-apyrase antibody
    • Ecto-ATP diphosphohydrolase 1 antibody
    • Ecto-ATPase 1 antibody
    • Ecto-ATPDase 1 antibody
    • Ectonucleoside triphosphate diphosphohydrolase 1 antibody
    • ENTP1_HUMAN antibody
    • ENTPD 1 antibody
    • ENTPD1 antibody
    • FLJ40921 antibody
    • FLJ40959 antibody
    • Lymphoid cell activation antigen antibody
    • NTPDase 1 antibody
    • NTPDase1 antibody
    • SPG64 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling CD39 with ab223843 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on endothelial cells of human pancreas (PMID: 18567823; PMID: 19308674). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue labeling CD39 with ab223843 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on stromal cells of human colon carcinoma (PMID: 25403716). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD39 with ab223843 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous and cytoplasmic staining on human tonsil (PMID: 27532024). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • All lanes : Anti-CD39 antibody [EPR20461] (ab223843) at 1/1000 dilution

    Lane 1 : Human fetal heart lysate at 20 µg
    Lane 2 : Human fetal spleen lysate at 20 µg
    Lane 3 : Human tonsil lysate at 20 µg
    Lane 4 : Human fetal kidney lysate at 10 µg

    Secondary
    Lanes 1-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Lane 4 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 58 kDa
    Observed band size: 78 kDa
    why is the actual band size different from the predicted?



    Exposure time : Lanes 1,4: 3 minutes;  Lanes 2-3: 10 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

References

ab223843 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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