Recombinant Anti-CD39 antibody [EPR20627] (ab223842)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20627] (ab223842)](https://www.abcam.com/ps/products/223/ab223842/Images/ab223842-285848-anti-cd39-antibody-epr20627-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20627] (ab223842)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20627] to CD39
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-CD39 antibody [EPR20627]
See all CD39 primary antibodies -
Description
Rabbit monoclonal [EPR20627] to CD39 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal heart, fetal spleen, placenta and tonsil lysates; Mouse placenta, brain, heart and kidney lysates. IHC-P: Human colon carcinoma, spleen and liver tissues; Mouse spleen tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20627 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab223842 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 78 kDa (predicted molecular weight: 57 kDa).
|
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| IHC-P | (5) |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 78 kDa (predicted molecular weight: 57 kDa). |
|
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Could also be implicated in the prevention of platelet aggregation. Hydrolyzes ATP and ADP equally well. -
Tissue specificity
Expressed primarily on activated lymphoid cells. Also expressed in endothelial tissues. The vascular isoform and the placental isoform II are present in both placenta and umbilical vein, whereas placental isoform I is present in placenta only. -
Sequence similarities
Belongs to the GDA1/CD39 NTPase family. -
Post-translational
modificationsThe N-terminus is blocked.
Palmitoylated in the N-terminal part. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 953 Human
- Entrez Gene: 12495 Mouse
- Omim: 601752 Human
- SwissProt: P49961 Human
- SwissProt: P55772 Mouse
- Unigene: 576612 Human
- Unigene: 2824 Mouse
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Form
There are 3 isoforms produced by alternative splicing. -
Alternative names
- ATPDase antibody
- CD 39 antibody
- CD39 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20627] (ab223842)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue labeling CD39 with ab223842 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on stromal cells of human colon carcinoma (PMID: 25403716). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Western blot - Anti-CD39 antibody [EPR20627] (ab223842)](https://www.abcam.com/ps/products/223/ab223842/Images/ab223842-285851-anti-cd39-antibody-epr20627-western-blot.jpg)
Western blot - Anti-CD39 antibody [EPR20627] (ab223842)Lanes 1-2 : Anti-CD39 antibody [EPR20627] (ab223842) at 1/1000 dilution
Lanes 3-5 : Anti-CD39 antibody [EPR20627] (ab223842) at 1/10000 dilution
Lane 1 : Human fetal heart lysate
Lane 2 : Human placenta lysate
Lane 3 : Human fetal spleen lysate
Lane 4 : Human tonsil lysate
Lane 5 : Mouse placenta lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 57 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted?Exposure time : Lane 1: 30 seconds; Lane 2: 3 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 18036594; PMID: 10636909).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20627] (ab223842)](https://www.abcam.com/ps/products/223/ab223842/Images/ab223842-285846-anti-cd39-antibody-epr20627-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20627] (ab223842)Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling CD39 with ab223842 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous and cytoplasmic staining on human spleen (PMID: 22684996; PMID: 27532024). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20627] (ab223842)](https://www.abcam.com/ps/products/223/ab223842/Images/ab223842-285845-anti-cd39-antibody-epr20627-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20627] (ab223842)Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD39 with ab223842 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on mouse spleen (PMID: 22684996; PMID: 27532024). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20627] (ab223842)](https://www.abcam.com/ps/products/223/ab223842/Images/ab223842-285849-anti-cd39-antibody-epr20627-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20627] (ab223842)Immunohistochemical analysis of paraffin-embedded human liver tissue labeling CD39 with ab223842 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on endothelial cells of human liver (PMID: 18567823; PMID: 19308674). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Western blot - Anti-CD39 antibody [EPR20627] (ab223842)](https://www.abcam.com/ps/products/223/ab223842/Images/ab223842-285850-anti-cd39-antibody-epr20627-western-blot.jpg)
Western blot - Anti-CD39 antibody [EPR20627] (ab223842)All lanes : Anti-CD39 antibody [EPR20627] (ab223842) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Mouse kidney lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 57 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted?Exposure times: Lane 1: 30 seconds; Lane 2: 3 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 18036594; PMID: 10636909).
-
![Anti-CD39 antibody [EPR20627] (ab223842)](https://www.abcam.com/ps/products/223/ab223842/Images/ab223842-7-benefits-of-recombinant-antibodies.png)
Anti-CD39 antibody [EPR20627] (ab223842)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab223842 has been referenced in 2 publications.
- Jin H et al. Increased Extracellular Adenosine in Radiotherapy-Resistant Breast Cancer Cells Enhances Tumor Progression through A2AR-Akt-ß-Catenin Signaling. Cancers (Basel) 13:N/A (2021). PubMed: 33925516
- Wu J et al. High Expression of CD39 is Associated with Poor Prognosis and Immune Infiltrates in Clear Cell Renal Cell Carcinoma. Onco Targets Ther 13:10453-10464 (2020). PubMed: 33116625