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    cd39-antibody-epr22507-48-ab227840.pdf

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Immunology Adaptive Immunity B Cells CD
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RecombinantRabMAb

Recombinant Anti-CD39 antibody [EPR22507-48] (ab227840)

  • Datasheet
  • Certificate of Compliance
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Western blot - Anti-CD39 antibody [EPR22507-48] (ab227840)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR22507-48] (ab227840)
  • Immunocytochemistry/ Immunofluorescence - Anti-CD39 antibody [EPR22507-48] (ab227840)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR22507-48] (ab227840)
  • Flow Cytometry - Anti-CD39 antibody [EPR22507-48] (ab227840)
  • Immunoprecipitation - Anti-CD39 antibody [EPR22507-48] (ab227840)
  • Anti-CD39 antibody [EPR22507-48] (ab227840)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22507-48] to CD39
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
  • Reacts with: Mouse

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Carrier Free PE

You may also be interested in

Protein
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Recombinant Human CD39 protein (ab114445)
Primary
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Anti-CD73 antibody [EPR6114] (ab133582)
Secondary
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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

View more associated products

Overview

  • Product name

    Anti-CD39 antibody [EPR22507-48]
    See all CD39 primary antibodies
  • Description

    Rabbit monoclonal [EPR22507-48] to CD39
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse placenta, heart and spleen lysate. IHC-P: Mouse liver and spleen tissue. ICC/IF: Mouse splenocytes. Flow Cyt: Mouse splenocytes. IP: Mouse placenta lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR22507-48
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • B Cells
    • CD
    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Immunology
    • Cell Type Markers
    • CD
    • NK Cells

Associated products

  • Alternative Versions

    • Anti-CD39 antibody [EPR22507-48] - BSA and Azide free (ab254006)
    • Alexa Fluor® 488 Anti-CD39 antibody [EPR22507-48] (ab270916)
  • Compatible Secondaries

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Immunohistochemistry kits

    • Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human CD39 protein (ab114445)
  • Related Products

    • Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab227840 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Detects a band of approximately 78 kDa (predicted molecular weight: 58 kDa).
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/100.
Flow Cyt
1/500.
IP
1/30.
Notes
WB
1/1000. Detects a band of approximately 78 kDa (predicted molecular weight: 58 kDa).
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/100.
Flow Cyt
1/500.
IP
1/30.

Target

  • Function

    In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Could also be implicated in the prevention of platelet aggregation. Hydrolyzes ATP and ADP equally well.
  • Tissue specificity

    Expressed primarily on activated lymphoid cells. Also expressed in endothelial tissues. The vascular isoform and the placental isoform II are present in both placenta and umbilical vein, whereas placental isoform I is present in placenta only.
  • Sequence similarities

    Belongs to the GDA1/CD39 NTPase family.
  • Post-translational
    modifications

    The N-terminus is blocked.
    Palmitoylated in the N-terminal part.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P49961 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 12495 Mouse
    • SwissProt: P55772 Mouse
    • Unigene: 2824 Mouse
    • Form

      There are 3 isoforms produced by alternative splicing.
    • Alternative names

      • ATPDase antibody
      • CD 39 antibody
      • CD39 antibody
      • CD39 antigen antibody
      • DKFZp686D194 antibody
      • DKFZp686I093 antibody
      • Ecto apyrase antibody
      • Ecto ATP diphosphohydrolase antibody
      • Ecto-apyrase antibody
      • Ecto-ATP diphosphohydrolase 1 antibody
      • Ecto-ATPase 1 antibody
      • Ecto-ATPDase 1 antibody
      • Ectonucleoside triphosphate diphosphohydrolase 1 antibody
      • ENTP1_HUMAN antibody
      • ENTPD 1 antibody
      • ENTPD1 antibody
      • FLJ40921 antibody
      • FLJ40959 antibody
      • Lymphoid cell activation antigen antibody
      • NTPDase 1 antibody
      • NTPDase1 antibody
      • SPG64 antibody
      see all

    Images

    • Western blot - Anti-CD39 antibody [EPR22507-48] (ab227840)
      Western blot - Anti-CD39 antibody [EPR22507-48] (ab227840)
      All lanes : Anti-CD39 antibody [EPR22507-48] (ab227840) at 1/1000 dilution

      Lane 1 : Mouse placenta lysate
      Lane 2 : Mouse heart lysate
      Lane 3 : Mouse spleen lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Lanes 1-2 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
      Lane 3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 58 kDa
      Observed band size: 78 kDa why is the actual band size different from the predicted?


      Exposure time: 81 seconds


      The molecular weight observed is consistent with what has been described in the literature (PMID:18036594).

      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR22507-48] (ab227840)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR22507-48] (ab227840)

      Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD39 with ab227840 at 1/500 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on mouse spleen (PMID: 18567823). The section was incubated with ab227840 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunocytochemistry/ Immunofluorescence - Anti-CD39 antibody [EPR22507-48] (ab227840)
      Immunocytochemistry/ Immunofluorescence - Anti-CD39 antibody [EPR22507-48] (ab227840)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse splenocytes labeling CD39 with ab227840 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in subsets of mouse splenocyte. The nuclear stain is DAPI (blue). Counterstained with Anti-CD45R/B220 Antibody (Alexa Fluor® 647)  at a 1/200 dilution (red).

      Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR22507-48] (ab227840)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR22507-48] (ab227840)

      Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling CD39 with ab227840 at 1/500 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on blood vessels of mouse liver (PMID: 18567823). The section was incubated with ab227840 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Flow Cytometry - Anti-CD39 antibody [EPR22507-48] (ab227840)
      Flow Cytometry - Anti-CD39 antibody [EPR22507-48] (ab227840)

      Flow cytometric analysis of mouse splenocytes labeling CD39 with ab227840 at 1/500. Cells were stained with rabbit IgG Isotype control (ab172730) (Left panel) or ab227840 (Right panel). Then stained with anti-CD45R conjugated to Alexa Fluor® 647. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

    • Immunoprecipitation - Anti-CD39 antibody [EPR22507-48] (ab227840)
      Immunoprecipitation - Anti-CD39 antibody [EPR22507-48] (ab227840)

      CD39 was immunoprecipitated from 0.35 mg mouse placenta lysate with ab227840 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab227840 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

      Lane 1: Mouse placenta lysate 10 µg (Input).
      Lane 2: ab227840 IP in mouse placenta lysate.
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab227840 in mouse placenta lysate.

      Blocking/Dilution buffer: 5% NFDM/TBST.
      Exposure time: 1 second.

    • Anti-CD39 antibody [EPR22507-48] (ab227840)
      Anti-CD39 antibody [EPR22507-48] (ab227840)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (1)

    Publishing research using ab227840? Please let us know so that we can cite the reference in this datasheet.

    ab227840 has been referenced in 1 publication.

    • Shih YV  et al. Dysregulation of ectonucleotidase-mediated extracellular adenosine during postmenopausal bone loss. Sci Adv 5:eaax1387 (2019). PubMed: 31457100

    Customer reviews and Q&As

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