Recombinant Anti-CD39 antibody [EPR22507-48] (ab227840)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-CD39 antibody [EPR22507-48]
See all CD39 primary antibodies -
Description
Rabbit monoclonal [EPR22507-48] to CD39 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IPmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment within Mouse CD39 aa 1-500. The exact sequence is proprietary.
Database link: P55772 -
Positive control
- WB: Mouse placenta, heart and spleen lysate. IHC-P: Mouse liver and spleen tissue. ICC/IF: Mouse splenocytes. Flow Cyt: Mouse splenocytes. IP: Mouse placenta lysate.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22507-48 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Immunohistochemistry kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab227840 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 78 kDa (predicted molecular weight: 58 kDa). | |
| IHC-P | 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. | |
| ICC/IF | 1/100. | |
| Flow Cyt | 1/500. | |
| IP | 1/30. |
Target
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Function
In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Could also be implicated in the prevention of platelet aggregation. Hydrolyzes ATP and ADP equally well. -
Tissue specificity
Expressed primarily on activated lymphoid cells. Also expressed in endothelial tissues. The vascular isoform and the placental isoform II are present in both placenta and umbilical vein, whereas placental isoform I is present in placenta only. -
Sequence similarities
Belongs to the GDA1/CD39 NTPase family. -
Post-translational
modificationsThe N-terminus is blocked.
Palmitoylated in the N-terminal part. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 12495 Mouse
- SwissProt: P55772 Mouse
- Unigene: 2824 Mouse
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Form
There are 3 isoforms produced by alternative splicing. -
Alternative names
- ATPDase antibody
- CD 39 antibody
- CD39 antibody
see all
Images
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![Western blot - Anti-CD39 antibody [EPR22507-48] (ab227840)](https://www.abcam.com/ps/products/227/ab227840/Images/ab227840-4-227840WB1.jpg)
Western blot - Anti-CD39 antibody [EPR22507-48] (ab227840)All lanes : Anti-CD39 antibody [EPR22507-48] (ab227840) at 1/1000 dilution
Lane 1 : Mouse placenta lysate
Lane 2 : Mouse heart lysate
Lane 3 : Mouse spleen lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 58 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted?
Exposure time: 81 secondsThe molecular weight observed is consistent with what has been described in the literature (PMID:18036594).
Blocking/Dilution buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR22507-48] (ab227840)](https://www.abcam.com/ps/products/227/ab227840/Images/ab227840-3-227840IHC2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR22507-48] (ab227840)Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD39 with ab227840 at 1/500 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on mouse spleen (PMID: 18567823). The section was incubated with ab227840 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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![Immunocytochemistry/ Immunofluorescence - Anti-CD39 antibody [EPR22507-48] (ab227840)](https://www.abcam.com/ps/products/227/ab227840/Images/ab227840-1-227840ICC1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD39 antibody [EPR22507-48] (ab227840)Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse splenocytes labeling CD39 with ab227840 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in subsets of mouse splenocyte. The nuclear stain is DAPI (blue). Counterstained with Anti-CD45R/B220 Antibody (Alexa Fluor® 647) at a 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR22507-48] (ab227840)](https://www.abcam.com/ps/products/227/ab227840/Images/ab227840-2-227840IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR22507-48] (ab227840)Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling CD39 with ab227840 at 1/500 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on blood vessels of mouse liver (PMID: 18567823). The section was incubated with ab227840 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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![Flow Cytometry - Anti-CD39 antibody [EPR22507-48] (ab227840)](https://www.abcam.com/ps/products/227/ab227840/Images/ab227840-5-227840FC1.jpg)
Flow Cytometry - Anti-CD39 antibody [EPR22507-48] (ab227840)Flow cytometric analysis of mouse splenocytes labeling CD39 with ab227840 at 1/500. Cells were stained with rabbit IgG Isotype control (ab172730) (Left panel) or ab227840 (Right panel). Then stained with anti-CD45R conjugated to Alexa Fluor® 647. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.
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![Immunoprecipitation - Anti-CD39 antibody [EPR22507-48] (ab227840)](https://www.abcam.com/ps/products/227/ab227840/Images/ab227840-6-227840IP1.jpg)
Immunoprecipitation - Anti-CD39 antibody [EPR22507-48] (ab227840)CD39 was immunoprecipitated from 0.35 mg mouse placenta lysate with ab227840 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab227840 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: Mouse placenta lysate 10 µg (Input).
Lane 2: ab227840 IP in mouse placenta lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab227840 in mouse placenta lysate.Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 1 second.
Datasheets and documents
References
ab227840 has not yet been referenced specifically in any publications.
