Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22507-67] to CD39
- Suitable for: WB
- Reacts with: Mouse, Rat
Product nameAnti-CD39 antibody [EPR22507-67]
See all CD39 primary antibodies
DescriptionRabbit monoclonal [EPR22507-67] to CD39
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
Species reactivityReacts with: Mouse, Rat
Recombinant fragment within Mouse CD39 aa 1-500. The exact sequence is proprietary.
Database link: P55772
- WB: Mouse heart and placenta lysates; rat spleen and heart lysates.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab252917 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 78 kDa (predicted molecular weight: 58 kDa).|
FunctionIn the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Could also be implicated in the prevention of platelet aggregation. Hydrolyzes ATP and ADP equally well.
Tissue specificityExpressed primarily on activated lymphoid cells. Also expressed in endothelial tissues. The vascular isoform and the placental isoform II are present in both placenta and umbilical vein, whereas placental isoform I is present in placenta only.
Sequence similaritiesBelongs to the GDA1/CD39 NTPase family.
modificationsThe N-terminus is blocked.
Palmitoylated in the N-terminal part.
- Information by UniProt
FormThere are 3 isoforms produced by alternative splicing.
- ATPDase antibody
- CD 39 antibody
- CD39 antibody
All lanes : Anti-CD39 antibody [EPR22507-67] (ab252917) at 1/1000 dilution
Lane 1 : Mouse heart lysate
Lane 2 : Mouse placenta lysate
Lane 3 : Rat spleen lysate
Lane 4 : Rat heart lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 58 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted?
The molecular weight observed is consistent with what has been described in the literature (PMID:18036594).
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 37 secs; Lane 2: 48 secs; Lanes 3-4: 3 mins.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab252917 has not yet been referenced specifically in any publications.