Overview

  • Product name

  • Description

    Rabbit polyclonal to CD3D
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CD3D aa 121-171. The exact sequence is proprietary.
    Database link: P04234

  • Positive control

    • WB: Jurkat whole cell lysate. IP: Jurkat whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab226246 was affinity purified using an epitope specific to CD3D immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab226246 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 19 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    The CD3 complex mediates signal transduction.
  • Involvement in disease

    Defects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
  • Sequence similarities

    Contains 1 ITAM domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Form

    .
  • Alternative names

    • CD3 antigen delta subunit antibody
    • CD3 delta antibody
    • CD3-delta antibody
    • CD3d antibody
    • CD3d antigen delta polypeptide (TiT3 complex) antibody
    • CD3d molecule antibody
    • CD3d molecule delta (CD3-TCR complex) antibody
    • CD3D_HUMAN antibody
    • IMD19 antibody
    • OKT3 delta chain antibody
    • T cell receptor T3 delta chain antibody
    • T-cell receptor T3 delta chain antibody
    • T-cell surface glycoprotein CD3 delta chain antibody
    • T3D antibody
    see all

Images

  • All lanes : Anti-CD3D antibody (ab226246) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen)
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 19 kDa


    Exposure time: 30 seconds


    Lysates prepared using NETN lysis buffer.

  • CD3D was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226246 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226246 at 1 µg/ml.

    Lane 1: ab226246 IP in Jurkat whole cell lysate.

    Lane 2: Control IgG IP in Jurkat whole cell lysate.

    Detection: Chemiluminescence with exposure time of 3 minutes.

References

ab226246 has not yet been referenced specifically in any publications.

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