Recombinant
RabMAb

Recombinant Anti-CD3D antibody [EPR20544] (ab213362)

Overview

  • Product name

    Anti-CD3D antibody [EPR20544]
    See all CD3D primary antibodies
  • Description

    Rabbit monoclonal [EPR20544] to CD3D
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IHC-Fr, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Synthetic peptide within Mouse CD3D aa 150 to the C-terminus (intracellular). The exact sequence is proprietary.
    Database link: P04235

  • Positive control

    • WB: Mouse thymus and lymph node lysates. IHC-P: Mouse spleen and thymus tissues. IHC-Fr: Mouse spleen tissue. Flow Cyt: Mouse spleen cells. IP: Mouse thymus lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20544
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab213362 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 24 kDa (predicted molecular weight: 19 kDa).
IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr 1/100.

Antigen retrieval: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20).

Flow Cyt 1/600.
IP 1/30.

Target

  • Function

    The CD3 complex mediates signal transduction.
  • Involvement in disease

    Defects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
  • Sequence similarities

    Contains 1 ITAM domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Form

    .
  • Alternative names

    • CD3 antigen delta subunit antibody
    • CD3 delta antibody
    • CD3-delta antibody
    • CD3d antibody
    • CD3d antigen delta polypeptide (TiT3 complex) antibody
    • CD3d molecule antibody
    • CD3d molecule delta (CD3-TCR complex) antibody
    • CD3D_HUMAN antibody
    • IMD19 antibody
    • OKT3 delta chain antibody
    • T cell receptor T3 delta chain antibody
    • T-cell receptor T3 delta chain antibody
    • T-cell surface glycoprotein CD3 delta chain antibody
    • T3D antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD3D with ab213362 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Cytoplasmic staining on T cells of mouse splenic periarterial lymphatic sheath is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse spleen tissue labeling CD3D with ab213362 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Cytoplasmic and membranous staining on mouse splenic periarterial lymphatic sheath is observed.

    The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

  • All lanes : Anti-CD3D antibody [EPR20544] (ab213362) at 1/1000 dilution

    Lane 1 : Mouse thymus lysate
    Lane 2 : Mouse lymph node lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 19 kDa
    Observed band size: 24 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 15 seconds; Lane 2: 3 minutes.

  • Flow cytometric analysis of mouse spleen cells labeling CD3D with ab213362 at 1/600 dilution (right panel), compared with a rabbit monoclonal IgG isotype control (ab172730) (left panel). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

    Firstly, cells surface was stained with anti-mouse CD3 Alexa Fluor 647 (Y axis), then cells were fixed with 4% PFA followed by intracellular staining with ab213362. The same population of cells were stained by both: anti-mouse CD3 and anti-mouse CD3D (ab213362) antibodies.

  • CD3D was immunoprecipitated from 0.35 mg of mouse thymus lysate with ab213362 at 1/30 dilution.

    Western blot was performed from the immunoprecipitate using ab213362 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

    Lane 1: Mouse thymus lysate, 10 µg (Input).

    Lane 2: ab213362 IP in mouse thymus lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab213362 in mouse thymus lysate .

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

  • Immunohistochemical analysis of paraffin-embedded mouse thymus tissue labeling CD3D with ab213362 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. 

    Cytoplasmic and membranous staining on T cells of mouse thymus medulla is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

ab213362 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab213362.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up