Recombinant
RabMAb

Recombinant Anti-CD3D antibody [EPR20544] - BSA and Azide free (ab229280)

Overview

  • Product name

    Anti-CD3D antibody [EPR20544] - BSA and Azide free
    See all CD3D primary antibodies
  • Description

    Rabbit monoclonal [EPR20544] to CD3D - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Fr, IP, Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Synthetic peptide within Mouse CD3D aa 150 to the C-terminus (intracellular). The exact sequence is proprietary.
    Database link: P04235

  • Positive control

    • WB: Mouse thymus and lymph node lysates. IHC-P: Mouse spleen and thymus tissues. IHC-Fr: Mouse spleen tissue. Flow Cyt: Mouse spleen cells. IP: Mouse thymus lysate.
  • General notes

    Ab229280 is the carrier-free version of ab213362. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab229280 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20544
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab229280 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 24 kDa (predicted molecular weight: 19 kDa).
IHC-Fr Use at an assay dependent concentration.

Antigen retrieval: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20).

IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    The CD3 complex mediates signal transduction.
  • Involvement in disease

    Defects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
  • Sequence similarities

    Contains 1 ITAM domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Form

    .
  • Alternative names

    • CD3 antigen delta subunit antibody
    • CD3 delta antibody
    • CD3-delta antibody
    • CD3d antibody
    • CD3d antigen delta polypeptide (TiT3 complex) antibody
    • CD3d molecule antibody
    • CD3d molecule delta (CD3-TCR complex) antibody
    • CD3D_HUMAN antibody
    • IMD19 antibody
    • OKT3 delta chain antibody
    • T cell receptor T3 delta chain antibody
    • T-cell receptor T3 delta chain antibody
    • T-cell surface glycoprotein CD3 delta chain antibody
    • T3D antibody
    see all

References

ab229280 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab229280.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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