Anti-CD3 epsilon antibody [PC3/188A] (ab219328)
Key features and details
- Mouse monoclonal [PC3/188A] to CD3 epsilon
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD3 epsilon antibody [PC3/188A]
See all CD3 epsilon primary antibodies -
Description
Mouse monoclonal [PC3/188A] to CD3 epsilon -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human CD3 epsilon aa 150 to the C-terminus. Cytoplasmic domain.
Database link: P07766 -
Positive control
- IHC-P: Human tonsil tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.05% BSA, PBS -
Concentration information loading...
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Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
PC3/188A -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab219328 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 23 kDa.
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Notes |
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IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 23 kDa. |
Target
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Function
The CD3 complex mediates signal transduction. -
Sequence similarities
Contains 1 Ig-like (immunoglobulin-like) domain.
Contains 1 ITAM domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 916 Human
- Omim: 186830 Human
- SwissProt: P07766 Human
- Unigene: 3003 Human
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Alternative names
- CD3 epsilon antibody
- CD3e antibody
- CD3e antigen antibody
see all
Images
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Anti-CD3 epsilon antibody [PC3/188A] (ab219328) at 2 µg/ml + Human thymus whole cell lysate
Predicted band size: 23 kDa -
Anti-CD3 epsilon antibody [PC3/188A] (ab219328) at 2 µg/ml + Jurkat cells (human T cell leukemia cell line from peripheral blood)
Predicted band size: 23 kDa -
Immunohistochemical analysis of formalin-fixed paraffin-embedded human tonsil tissue sections labeling CD3 epsilon with ab219328 at 2 µg/mL.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab219328 has not yet been referenced specifically in any publications.