• Product name

    Anti-CD3G antibody [EPR4516]
    See all CD3G primary antibodies
  • Description

    Rabbit monoclonal [EPR4516] to CD3G
  • Host species

  • Tested applications

    Suitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CD3G aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • Human tonsil, Human fetal thymus, HuT-78 and Jurkat whole cell lysate (ab7899); Human tonsil tissue
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.


  • Form

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

  • Clone number

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab108996 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 20 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen retrieval is recommended.

ICC 1/500 - 1/1000.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      The CD3 complex mediates signal transduction.
    • Sequence similarities

      Contains 1 Ig-like (immunoglobulin-like) domain.
      Contains 1 ITAM domain.
    • Domain

      A di-leucine motif and a tyrosine-based motif are individually sufficient to induce both endocytosis and delivery to lysosomes.
    • Cellular localization

    • Information by UniProt
    • Database links

    • Alternative names

      • CD3 gamma antibody
      • CD3-GAMMA antibody
      • CD3g antibody
      • CD3g antigen gamma polypeptide (TiT3 complex) antibody
      • CD3g antigen gamma polypeptide antibody
      • CD3g molecule gamma (CD3 TCR complex) antibody
      • CD3g molecule gamma antibody
      • CD3G_HUMAN antibody
      • FLJ17620 antibody
      • FLJ17664 antibody
      • FLJ79544 antibody
      • FLJ94613 antibody
      • MGC138597 antibody
      • T cell antigen receptor complex gamma subunit of T3 antibody
      • T-cell receptor T3 gamma chain antibody
      • T-cell surface glycoprotein CD3 gamma chain antibody
      • T3G antibody
      see all


    • All lanes : Anti-CD3G antibody [EPR4516] (ab108996) at 1/1000 dilution

      Lane 1 : Human tonsil lysate
      Lane 2 : Human fetal thymus lysate
      Lane 3 : HuT-78 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 20 kDa

    • Anti-CD3G antibody [EPR4516] (ab108996) at 1/1000 dilution + Jurkat cell lysate at 10 µg

      Predicted band size: 20 kDa

    • ab108996, at 1/100 dilution, staining CD3G in paraffin-embedded Human tonsil tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


    This product has been referenced in:

    • Pérez-Mazliah D  et al. Follicular Helper T Cells are Essential for the Elimination of Plasmodium Infection. EBioMedicine 24:216-230 (2017). Read more (PubMed: 28888925) »
    See 1 Publication for this product

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