• Product name
    Anti-CD4 antibody [10C12]
    See all CD4 primary antibodies
  • Description
    Mouse monoclonal [10C12] to CD4
  • Host species
  • Tested applications
    Suitable for: ELISA, WB, ICC/IF, Blockingmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Cat, Dog, Pig, Chimpanzee, Monkey
  • Immunogen

    Synthetic peptide: LS EKKTCQCPHR FQKTCSPI, corresponding to C terminal amino acids 439-458 of Human CD4.

  • Epitope
    This antibody recognizes an epitope within amino acids 414-433 of the cytoplasmic tail region of human CD4.
  • Positive control
    • Jurkat and H9 cell lysates for ELISA, and CEM cells on glass slides for IF.
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab133487 as a replacement.



Our Abpromise guarantee covers the use of ab25804 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 10 - 20 µg/ml.
WB Use a concentration of 5 - 10 µg/ml. Predicted molecular weight: 51 kDa.
ICC/IF Use at an assay dependent concentration. PubMed: 20929345
Blocking Use at an assay dependent concentration. PubMed: 22745469


  • Function
    Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
  • Sequence similarities
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
  • Cellular localization
    Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 4 antibody
    • CD4 (L3T4) antibody
    • CD4 antibody
    • CD4 antigen (p55) antibody
    • CD4 antigen antibody
    • CD4 molecule antibody
    • CD4 receptor antibody
    • CD4+ Lymphocyte deficiency, included antibody
    • CD4_HUMAN antibody
    • CD4mut antibody
    • L3T4 antibody
    • Leu3 antibody
    • Ly-4 antibody
    • Lymphocyte antigen CD4 antibody
    • MGC165891 antibody
    • OTTHUMP00000238897 antibody
    • p55 antibody
    • T cell antigen T4 antibody
    • T cell antigen T4/LEU3 antibody
    • T cell differentiation antigen L3T4 antibody
    • T cell OKT4 deficiency, included antibody
    • T cell surface antigen T4/Leu 3 antibody
    • T cell surface antigen T4/Leu3 antibody
    • T cell surface glycoprotein CD4 antibody
    • T-cell surface antigen T4/Leu-3 antibody
    • T-cell surface glycoprotein CD4 antibody
    • W3/25 antibody
    • W3/25 antigen antibody
    see all


This product has been referenced in:
  • Chae JJ  et al. Nictitating membrane fixation improves stability of the contact lens on the animal corneal surface. PLoS One 13:e0194795 (2018). Read more (PubMed: 29584737) »
  • Wongsena W  et al. Melatonin suppresses eosinophils and Th17 cells in hamsters treated with a combination of human liver fluke infection and a chemical carcinogen. Pharmacol Rep 70:98-105 (2018). Read more (PubMed: 29331794) »

See all 6 Publications for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Western blot
Bos taurus Tissue lysate - whole (Spleen)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
45 µg
Noninfected controls and virally infected samples
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 24°C

Dr. Katie Knapek

Verified customer

Submitted Mar 22 2018

Abcam has not validated the combination of species/application used in this Abreview.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Mouse Tissue sections (small intestine)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate Buffer
small intestine
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Dr. Hsiao-Ying chiu

Verified customer

Submitted Dec 22 2014

Vielen Dank für Ihre Antwort und für diese weiteren Informationen.

Es tut mir leid zu hören, dass unsere Vorschläge in diesem Fall nicht zu einer Verbesserung Ihrer Ergebnisse geführt haben. Ich weiß, dass Sie ...

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Thank you for contacting Abcam and for your interest in our products.

Regarding the CD4 antibody ab25804, based on a BLAST of the immunogen, there is no homology with rat, therefore I would not recommend this antibody to use with rat samples...

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Thank you for your email.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with ab846.
To check th...

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Thank you for your reply.
For PPAR alpha, I would recommend ab27478 as an isotype control.

As for the CD4 antibody, your protocol seems to be fine. The immunogen sequence is ...

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Thank you for contacting us.
To answer your questions:
1. Yes, a negative control can be ommiting the primary. Other options could be using an isotype control antibody that compares with the priamry, or the blocking peptide. There are various...

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Thank you for your inquiry. Unfortunately, we do not offer an antibody that is tested and guaranteed for rabbit and IHC-P. We do not know if ab25804 is suitable for IHC-P. I would like to offer you a testing discount for this, but testing two unknowns ...

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Thank you for calling Abcam. I have continued to look through our catalogue for antibodies for CD4 and CD19, that are guaranteed to work in ICC/IF on mouse tissue. I have not found anything that is guaranteed to work in both your requirements, the best...

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Thank you for calling Abcam on Friday. For CD4, I have found the following set Protein: ab111463, fragment from 29-390. Antibodies: ab67480, aa7****                      ab51312, aa******* I know that the antibodies bind very close to each other, but t...

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1-10 of 12 Abreviews or Q&A


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