Overview

  • Product name

    Anti-CD4 antibody [B-A1] (FITC)
    See all CD4 primary antibodies
  • Description

    Mouse monoclonal [B-A1] to CD4 (FITC)
  • Host species

    Mouse
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human, Cynomolgus monkey
  • Immunogen

    PHA activated T cells

  • General notes

    This product was changed from ascites to tissue culture supernatant on 19th June 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab59474 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab91362 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
  • Sequence similarities

    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications

    Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
  • Cellular localization

    Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
  • Information by UniProt
  • Database links

    • Alternative names

      • CD 4 antibody
      • CD4 (L3T4) antibody
      • CD4 antibody
      • CD4 antigen (p55) antibody
      • CD4 antigen antibody
      • CD4 molecule antibody
      • CD4 receptor antibody
      • CD4+ Lymphocyte deficiency, included antibody
      • CD4_HUMAN antibody
      • CD4mut antibody
      • L3T4 antibody
      • Leu3 antibody
      • Ly-4 antibody
      • Lymphocyte antigen CD4 antibody
      • MGC165891 antibody
      • OTTHUMP00000238897 antibody
      • p55 antibody
      • T cell antigen T4 antibody
      • T cell antigen T4/LEU3 antibody
      • T cell differentiation antigen L3T4 antibody
      • T cell OKT4 deficiency, included antibody
      • T cell surface antigen T4/Leu 3 antibody
      • T cell surface antigen T4/Leu3 antibody
      • T cell surface glycoprotein CD4 antibody
      • T-cell surface antigen T4/Leu-3 antibody
      • T-cell surface glycoprotein CD4 antibody
      • W3/25 antibody
      • W3/25 antigen antibody
      see all

    Images

    • A typical staining pattern with the B-A1 monoclonal antibody of lymphocytes.

      This image was generated using the ascites version of the product.

    • Flow cytometry analysis of Cynomolgus Monkey whole blood after red blod cell lysis, staining CD4 with ab59474. Cells were fixed with paraformaldehyde. The sample was incubated with the primary antibody (1/100 in 1% BSA in PBS) for 1 hour at 4&degC.

      Gating Strategy: Live lymphocytes

      This image was generated using the ascites version of the product.

      See Abreview

    References

    This product has been referenced in:

    • Sui M  et al. Establishment of specific cytotoxic T lymphocyte culture system and its inhibitory effect on ovarian cancer. Oncol Lett 12:4087-4093 (2016). Flow Cyt ; Human . Read more (PubMed: 27895776) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-6 of 6 Abreviews or Q&A

    Application
    Flow Cytometry
    Sample
    Rat Cell (Splenocytes)
    Specification
    Splenocytes
    Preparation
    Cell harvesting/tissue preparation method: isolated from spleen
    Sample buffer: PBS/BSA 1%
    Permeabilization
    No
    Gating Strategy
    alive lymphocytes

    Abcam user community

    Verified customer

    Submitted Jan 10 2013

    Question
    Answer



    500 uL of the antibody is provided per 50-test vial.

    Read More
    Application
    Flow Cytometry
    Sample
    Human Cell (from buffy coat)
    Specification
    from buffy coat
    Preparation
    Cell harvesting/tissue preparation method: after ficoll extraction
    Sample buffer: PBS/BSA 1%
    Fixation
    none
    Permeabilization
    No
    Gating Strategy
    alive lymphocytes

    Abcam user community

    Verified customer

    Submitted Jan 04 2013

    Application
    Flow Cytometry
    Sample
    Mouse Cell (Splenocytes)
    Specification
    Splenocytes
    Preparation
    Cell harvesting/tissue preparation method: isolated from spleen
    Sample buffer: PBS/BSA 1%
    Permeabilization
    No
    Gating Strategy
    alive lymphocytes

    Abcam user community

    Verified customer

    Submitted Jan 02 2013

    Application
    Flow Cytometry
    Sample
    Cynomolgus Monkey Cell (from whole blood)
    Specification
    from whole blood
    Preparation
    Cell harvesting/tissue preparation method: after red blood cell lysis
    Sample buffer: PBS/BSA 1%
    Fixation
    Paraformaldehyde
    Permeabilization
    No
    Gating Strategy
    alive lymphocytes

    Abcam user community

    Verified customer

    Submitted Jan 02 2013

    Answer

    Thank you for contacting Abcam.
    All of the antibodies that you mentioned and all the antibodies in our catalogue are guaranteed for 6 months under our Abpromise. In terms of stability, they should all have be the same, regardless of the BSA concentration. As all of the antibodies you mentioned are recommended to sore at 4C, they should all remain stable for at least one year.
    If there is anything else I can help you with, please let me know.

    Read More

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