Anti-CD4 antibody [CAL4] (ab237722)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-CD4 antibody [CAL4]
See all CD4 primary antibodies -
DescriptionRabbit monoclonal [CAL4] to CD4
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-Pmore details
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Species reactivityReacts with: Mouse
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Immunogen
Synthetic peptide within Mouse CD4 aa 417-457. The exact sequence is proprietary.
Database link: P06332-1 -
Positive control
- IHC-P: Mouse spleen tissue.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.50
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
PurityProtein A purified
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Purification notesPurity >99%.
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ClonalityMonoclonal
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Clone numberCAL4
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab237722 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 0.5 µg/ml. Deparaffinize slides using xylene or xylene alternative and graded alcohols. Boil tissue section in TRIS EDTA buffer for 24 minutes followed by cooling at room temperature for 30-45 minutes. Incubate with primary antibody for 75 minutes at room temperature. Slides must be washed in between steps. Rinse slides with TBS/0.05% Tween. |
Target
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FunctionAccessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
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Sequence similaritiesContains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsPalmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts. -
Cellular localizationCell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
- Information by UniProt
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Database links
- Entrez Gene: 12504 Mouse
- SwissProt: P06332 Mouse
- Unigene: 2209 Mouse
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Alternative names
- CD 4 antibody
- CD4 (L3T4) antibody
- CD4 antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [CAL4] (ab237722)](https://www.abcam.com/ps/products/237/ab237722/Images/ab237722-315208-ab237722.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [CAL4] (ab237722)Formalin-fixed, paraffin-embedded mouse spleen tissue stained for CD4 using ab237722 at 0.5 µg/ml in immunohistochemical analysis.
Datasheets and documents
References
ab237722 has not yet been referenced specifically in any publications.
