Recombinant Anti-CD4 antibody [Campath-9H] - Chimeric (ab275975)
![Flow Cytometry - Anti-CD4 antibody [Campath-9H] (ab275975)](https://www.abcam.com/ps/products/275/ab275975/Images/ab275975-3-anti-CD4-antibody-FC.jpg "Flow Cytometry - Anti-CD4 antibody [Campath-9H] (ab275975)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Campath-9H] to CD4 - Chimeric
- Suitable for: Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-CD4 antibody [Campath-9H] - Chimeric
See all CD4 primary antibodies -
Description
Rabbit monoclonal [Campath-9H] to CD4 - Chimeric -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human CD4. Rat T-cell line (NB2) transfected with the human CD4.
Database link: P01730 -
Positive control
- Flow cyt: Human lymphocytes.
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General notes
This chimeric rabbit antibody was made using the variable domain sequences of the original rat IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Proclin 300
Constituent: 99.98% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
Campath-9H -
Isotype
IgG -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab275975 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use a concentration of 1 µg/ml.
|
| Notes |
|---|
|
Flow Cyt
Use a concentration of 1 µg/ml. |
Target
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Function
Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. -
Sequence similarities
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsPalmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts. -
Cellular localization
Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 920 Human
- Omim: 186940 Human
- SwissProt: P01730 Human
- Unigene: 631659 Human
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Alternative names
- CD 4 antibody
- CD4 (L3T4) antibody
- CD4 antibody
see all
Images
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Flow Cytometry - Anti-CD4 antibody [Campath-9H] (ab275975)
Flow cytometry of human lymphocytes labeling CD4 with ab275975 (panel B) compared with an an isotype control (panel A) at 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150073) and cells analysed on a FACSCanto flow-cytometer.
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![Anti-CD4 antibody [Campath-9H] - Chimeric (ab275975)](https://www.abcam.com/ps/products/275/ab275975/Images/ab275975-4-benefits-of-recombinant-antibodies.png)
Anti-CD4 antibody [Campath-9H] - Chimeric (ab275975)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab275975 has not yet been referenced specifically in any publications.