Anti-CD4 antibody [EPR19514] (ab183685)
- Datasheet
- References (16)
- Protocols
Overview
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Product nameAnti-CD4 antibody [EPR19514]
See all CD4 primary antibodies -
DescriptionRabbit monoclonal [EPR19514] to CD4
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Host speciesRabbit
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Tested applicationsSuitable for: IP, IHC-P, IHC-Fr, WBmore details
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Species reactivityReacts with: Mouse
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Immunogen
Recombinant fragment within Mouse CD4 aa 1-400. The exact sequence is proprietary.
Database link: P06332 -
Positive control
- WB: Mouse thymus lysate. IHC-P: Mouse spleen, colon and thymus tissues. IHC-Fr: Mouse spleen tissue. IP: Mouse thymus whole cell lysate.
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General notes
Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit Alexa Fluor® 488 (ab150077).
See other anti-rabbit secondary antibodies that can be used with this antibody.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEPR19514
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab183685 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | 1/40. | |
| IHC-P | 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. | |
| IHC-Fr | 1/200. | |
| WB | 1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa). |
Target
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FunctionAccessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
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Sequence similaritiesContains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsPalmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts. -
Cellular localizationCell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
- Information by UniProt
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Database links
- Entrez Gene: 12504 Mouse
- SwissProt: P06332 Mouse
- Unigene: 2209 Mouse
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Alternative names
- CD 4 antibody
- CD4 (L3T4) antibody
- CD4 antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR19514] (ab183685)](https://www.abcam.com/ps/products/183/ab183685/Images/ab183685-258194-183685IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR19514] (ab183685)Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling CD4 with ab183685 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.Membrane staining on T cells is observed.Counter stained with Hematoxylin.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution. -
![Immunohistochemistry (Frozen sections) - Anti-CD4 antibody [EPR19514] (ab183685)](https://www.abcam.com/ps/products/183/ab183685/Images/ab183685-258191-183685IHCIF.jpg)
Immunohistochemistry (Frozen sections) - Anti-CD4 antibody [EPR19514] (ab183685)Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse spleen tissue labeling CD4 with ab183685 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
The result showed membrane staining on mouse spleen.
The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.
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![Western blot - Anti-CD4 antibody [EPR19514] (ab183685)](https://www.abcam.com/ps/products/183/ab183685/Images/ab183685-258195-183685-wb.jpg)
Western blot - Anti-CD4 antibody [EPR19514] (ab183685)All lanes : Anti-CD4 antibody [EPR19514] (ab183685) at 1/2000 dilution
Lane 1 : Mouse thymus lysate
Lane 2 : Mouse liver tissue lysate
Lane 3 : Mouse brain tissue lysate
Lane 4 : Mouse kidney tissue lysate
Lane 5 : Raw264.7 (mouse abelson murine leukemia virus-induced tumor) whole cell lysate
Lane 6 : NIH/3T3 (mouse embryo) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 51 kDa
Observed band size: 51 kDaThe expression profile observed is consistent with what has been described in the literature (PMID: 2155425).
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1:3 seconds; Lanes 2,3,4,5 and 6:30 seconds
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![Immunoprecipitation - Anti-CD4 antibody [EPR19514] (ab183685)](https://www.abcam.com/ps/products/183/ab183685/Images/ab183685-258190-183685IP.jpg)
Immunoprecipitation - Anti-CD4 antibody [EPR19514] (ab183685)CD4 was immunoprecipitated from 1mg of Mouse thymus whole cell lysate with ab183685 at 1/40 dilution.
Western blot was performed from the immunoprecipitate using ab183685 at 1/1000 dilution.
VeriBlot for IP secondary antibody (HRP) (ab131366), was used as secondary antibody at 1/10000 dilution.
Lane 1: Mouse thymus whole cell lysate, 10µg (Input).
Lane 2: ab183685 IP in Mouse thymus whole cell lysate.
Lane 3: Rabbit IgG,monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab183685 in Mouse thymus whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR19514] (ab183685)](https://www.abcam.com/ps/products/183/ab183685/Images/ab183685-258193-183685IHCb.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR19514] (ab183685)Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling CD4 with ab183685 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.Membrane staining on lymphocytes and negative on epithelium cells of mouse colon is observed.Counter stained with Hematoxylin.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR19514] (ab183685)](https://www.abcam.com/ps/products/183/ab183685/Images/ab183685-306460-18368557043.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR19514] (ab183685)This image is courtesy of an anonymous Abreview.10% NBF-fixed, paraffin-embedded mouse spleen tissue stained for CD4 using ab183685 at 1/2000 dilution in immunohistochemical analysis, followed by Goat anti-Rabbit IgG Alexa Fluor® 647.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR19514] (ab183685)](https://www.abcam.com/ps/products/183/ab183685/Images/ab183685-258192-183685IHCc.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR19514] (ab183685)Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling CD4 with ab183685 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Negative on mouse cerebrum.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR19514] (ab183685)](https://www.abcam.com/ps/products/183/ab183685/Images/ab183685-323689-183685589711.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR19514] (ab183685)This image is courtesy of an Abreview submitted by Mr. Carl Hobbs.Formaldehyde-fixed, paraffin-embedded mouse spleen tissue stained for CD4 using ab183685 at 1/2000 dilution in immunohistochemical analysis, followed by Goat anti Rabbit IgG Biotin.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR19514] (ab183685)](https://www.abcam.com/ps/products/183/ab183685/Images/ab183685-306459-18368564045.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR19514] (ab183685)This image is courtesy of an anonymous Abreview.Formaldehyde-fixed, paraffin-embedded mouse thymus tissue stained for CD4 using ab183685 at 1/500 dilution in immunohistochemical analysis.
References
This product has been referenced in:
- Wu X et al. The ARE-binding protein Tristetraprolin (TTP) is a novel target and mediator of calcineurin tumor suppressing function in the skin. PLoS Genet 14:e1007366 (2018). Read more (PubMed: 29723192) »
- Guo JZ et al. The role of metallothionein in a dinitrofluorobenzene-induced atopic dermatitis-like murine model. Sci Rep 8:11129 (2018). Read more (PubMed: 30042397) »
