Name: Anti-CD4 antibody [EPR19514]
Brand: RabMAb
SKU: ab183685
Rating: 5 (12 reviews)

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Primary

Secondary

This product Rabbit Anti-CD4 antibody [EPR19514] (ab183685)
IP, IHC-P, IHC-Fr, WB

Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
IHC-P, WB, ELISA, IP

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Product name

Anti-CD4 antibody [EPR19514]
See all CD4 primary antibodies
Description

Rabbit monoclonal [EPR19514] to CD4
Host species

Rabbit
Tested applications

Suitable for: IP, IHC-P, IHC-Fr, WBmore details
Species reactivity

Reacts with: Mouse
Immunogen

Recombinant fragment within Mouse CD4 aa 1-400. The exact sequence is proprietary.
Database link: P06332

Run BLAST with Run BLAST with
Positive control
- WB: Mouse thymus lysate. IHC-P: Mouse spleen, colon and thymus tissues. IHC-Fr: Mouse spleen tissue. IP: Mouse thymus whole cell lysate.
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19514
Isotype

IgG
Research areas

Alternative Versions
- Anti-CD4 antibody [EPR19514] - Low endotoxin, Azide free (ab221775)
- Anti-CD4 antibody [EPR19514] (Alexa Fluor® 647) (ab225310)
Compatible Secondaries
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant human CD4 protein (Active) (ab167756)
Related Products
- Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)
- VeriBlot for IP Detection Reagent (HRP) (ab131366)

Our Abpromise guarantee covers the use of ab183685 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
IP		1/40.
IHC-P		1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr		1/200.
WB		1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).

Function

Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
Sequence similarities

Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Post-translational
modifications

Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
Cellular localization

Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
Target information above from: UniProt accession P01730 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 12504 Mouse
- SwissProt: P06332 Mouse
- Unigene: 2209 Mouse
Alternative names
- CD 4 antibody
- CD4 (L3T4) antibody
- CD4 antibody
- CD4 antigen (p55) antibody
- CD4 antigen antibody
- CD4 molecule antibody
- CD4 receptor antibody
- CD4+ Lymphocyte deficiency, included antibody
- CD4_HUMAN antibody
- CD4mut antibody
- L3T4 antibody
- Leu3 antibody
- Ly-4 antibody
- Lymphocyte antigen CD4 antibody
- MGC165891 antibody
- OTTHUMP00000238897 antibody
- p55 antibody
- T cell antigen T4 antibody
- T cell antigen T4/LEU3 antibody
- T cell differentiation antigen L3T4 antibody
- T cell OKT4 deficiency, included antibody
- T cell surface antigen T4/Leu 3 antibody
- T cell surface antigen T4/Leu3 antibody
- T cell surface glycoprotein CD4 antibody
- T-cell surface antigen T4/Leu-3 antibody
- T-cell surface glycoprotein CD4 antibody
- W3/25 antibody
- W3/25 antigen antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR19514] (ab183685)

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling CD4 with ab183685 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Membrane staining on T cells is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Immunohistochemistry (Frozen sections) - Anti-CD4 antibody [EPR19514] (ab183685)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse spleen tissue labeling CD4 with ab183685 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

The result showed membrane staining on mouse spleen.

The nuclear counterstain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.
Western blot - Anti-CD4 antibody [EPR19514] (ab183685)

All lanes : Anti-CD4 antibody [EPR19514] (ab183685) at 1/2000 dilution

Lane 1 : Mouse thymus lysate
Lane 2 : Mouse liver tissue lysate
Lane 3 : Mouse brain tissue lysate
Lane 4 : Mouse kidney tissue lysate
Lane 5 : Raw264.7 (mouse abelson murine leukemia virus-induced tumor) whole cell lysate
Lane 6 : NIH/3T3 (mouse embryo) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 51 kDa
Observed band size: 51 kDa

The expression profile observed is consistent with what has been described in the literature (PMID: 2155425).

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1:3 seconds; Lanes 2,3,4,5 and 6:30 seconds
Immunoprecipitation - Anti-CD4 antibody [EPR19514] (ab183685)

CD4 was immunoprecipitated from 1mg of Mouse thymus whole cell lysate with ab183685 at 1/40 dilution.

Western blot was performed from the immunoprecipitate using ab183685 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: Mouse thymus whole cell lysate, 10µg (Input).

Lane 2: ab183685 IP in Mouse thymus whole cell lysate.

Lane 3: Rabbit IgG,monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab183685 in Mouse thymus whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 5 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR19514] (ab183685)

Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling CD4 with ab183685 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Membrane staining on lymphocytes and negative on epithelium cells of mouse colon is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR19514] (ab183685)This image is courtesy of an anonymous Abreview.

10% NBF-fixed, paraffin-embedded mouse spleen tissue stained for CD4 using ab183685 at 1/2000 dilution in immunohistochemical analysis, followed by Goat anti-Rabbit IgG Alexa Fluor^® 647.
See Abreview
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR19514] (ab183685)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling CD4 with ab183685 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Negative on mouse cerebrum.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR19514] (ab183685)This image is courtesy of an Abreview submitted by Mr. Carl Hobbs.

Formaldehyde-fixed, paraffin-embedded mouse spleen tissue stained for CD4 using ab183685 at 1/2000 dilution in immunohistochemical analysis, followed by Goat anti Rabbit IgG Biotin.
See Abreview
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR19514] (ab183685)This image is courtesy of an anonymous Abreview.

Formaldehyde-fixed, paraffin-embedded mouse thymus tissue stained for CD4 using ab183685 at 1/500 dilution in immunohistochemical analysis.
See Abreview

Immunoprecipitation protocols
Immunohistochemistry protocols
Western blot protocols

This product has been referenced in:

Camilio KA et al. Combining the oncolytic peptide LTX-315 with doxorubicin demonstrates therapeutic potential in a triple-negative breast cancer model. Breast Cancer Res 21:9 (2019). Read more (PubMed: 30670061) »
Hosomi S et al. Myeloid-Derived Suppressor Cells Infiltrate the Brain and Suppress Neuroinflammation in a Mouse Model of Focal Traumatic Brain Injury. Neuroscience 406:457-466 (2019). Read more (PubMed: 30880103) »

See all 19 Publications for this product

Publishing research using ab183685? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-10 of 12 Abreviews or Q&A

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (Spleen)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Leica ER2 solution

Permeabilization

Specification

Spleen

Fixative

Formaldehyde

Zohar Gavish

Verified customer

Submitted May 13 2019

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (B16F10 (melanoma) syngeneic mouse tumor)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Tris/EDTA

Permeabilization

Yes - 3% H2O2

Specification

B16F10 (melanoma) syngeneic mouse tumor

Fixative

Formaldehyde

Abcam user community

Verified customer

Submitted Apr 30 2019

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (lungs)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6

Permeabilization

Specification

lungs

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Fixative

Formaldehyde

Abcam user community

Verified customer

Submitted Nov 28 2018

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (brain tissue)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Tris/EDTA ph 9

Permeabilization

Specification

brain tissue

Blocking step

Normal Goat Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C

Fixative

Formaldehyde

Herr Dr. Markus Kipp

Verified customer

Submitted Aug 21 2018

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (Eo771 mammary tumor)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citrate Buffer, Vector laboratories(H-3300)

Permeabilization

Yes - 0.2% Triton-X

Specification

Eo771 mammary tumor

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C

Fixative

Formalin

Anugraha Rajagopalan

Verified customer

Submitted Apr 11 2018

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (Thymus)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Bond TM Epitope Retrieval 2-1L (Leika Biosystems)

Permeabilization

Specification

Thymus

Fixative

Formaldehyde

Abcam user community

Verified customer

Submitted Mar 27 2018

Application

Western blot

Sample

Mouse Cell lysate - whole cell (mouse hepatocytes)

Gel Running Conditions

Reduced Denaturing

Loading amount

25 µg

Specification

mouse hepatocytes

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Jan 10 2018

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (Colon)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: pH 9.0 EDTA

Permeabilization

Specification

Colon

Blocking step

Protein Block ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C

Fixative

Formaldehyde

Abcam user community

Verified customer

Submitted Dec 07 2017

Application

Immunohistochemistry (Frozen sections)

Sample

Mouse Tissue sections (Liver)

Permeabilization

Yes - 0.1% tween, trition 0.3% in PBS

Specification

Liver

Blocking step

BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C

Fixative

Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 30 2017

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (Spleen)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citric acid

Permeabilization

Specification

Spleen

Blocking step

BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C

Fixative

Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted Mar 27 2017

1-10 of 12 Abreviews or Q&A

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Recombinant Anti-CD4 antibody [EPR19514] (ab183685)

Overview

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Datasheets and documents

References

This product has been referenced in:

Customer reviews and Q&As

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD4 antibody [EPR19514]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD4 antibody [EPR19514]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD4 antibody [EPR19514]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD4 antibody [EPR19514]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD4 antibody [EPR19514]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD4 antibody [EPR19514]

Western blot abreview for Anti-CD4 antibody [EPR19514]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD4 antibody [EPR19514]

Immunohistochemistry (Frozen sections) abreview for Anti-CD4 antibody [EPR19514]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD4 antibody [EPR19514]