Recombinant Anti-CD4 antibody [EPR20122] (ab207755)
![Flow Cytometry - Anti-CD4 antibody [EPR20122] (ab207755)](https://www.abcam.com/ps/products/207/ab207755/Images/ab207755-269498-anti-cd4-antibody-epr20122-flow-cytometry.jpg "Flow Cytometry - Anti-CD4 antibody [EPR20122] (ab207755)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20122] to CD4
- Suitable for: WB, Flow Cyt, IP
- Reacts with: Mouse
Overview
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Product name
Anti-CD4 antibody [EPR20122]
See all CD4 primary antibodies -
Description
Rabbit monoclonal [EPR20122] to CD4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt, IPmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment within Mouse CD4 aa 1-400. The exact sequence is proprietary.
Database link: P06332 -
Positive control
- WB: Mouse thymus lysate. Flow Cyt: Mouse thymocytes. IP: Mouse thymus lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20122 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab207755 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa). | |
| Flow Cyt | 1/60. | |
| IP | 1/30. |
Target
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Function
Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. -
Sequence similarities
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsPalmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts. -
Cellular localization
Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 12504 Mouse
- SwissProt: P06332 Mouse
- Unigene: 2209 Mouse
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Alternative names
- CD 4 antibody
- CD4 (L3T4) antibody
- CD4 antibody
see all
Images
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Flow Cytometry - Anti-CD4 antibody [EPR20122] (ab207755)
Flow cytometric analysis of Mouse thymocytes labeling CD4 with ab207755 at 1/60 dilution (red), compared with ab172730 (Rabbit IgG, monoclonal [EPR25A] - Isotype Control) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
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![Western blot - Anti-CD4 antibody [EPR20122] (ab207755)](https://www.abcam.com/ps/products/207/ab207755/Images/ab207755-269692-anti-cd4-antibody-epr20122-western-blot.jpg)
Western blot - Anti-CD4 antibody [EPR20122] (ab207755)Anti-CD4 antibody [EPR20122] (ab207755) at 1/5000 dilution + Mouse thymus at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 51 kDa
Exposure time: 5 secondsBlocking and Diluting buffer and concentration: 5% NFDM/TBST.
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![Immunoprecipitation - Anti-CD4 antibody [EPR20122] (ab207755)](https://www.abcam.com/ps/products/207/ab207755/Images/ab207755-269497-anti-cd4-antibody-epr20122-immunoprecipitation.jpg)
Immunoprecipitation - Anti-CD4 antibody [EPR20122] (ab207755)CD4 was immunoprecipitated from 0.35 mg of Mouse thymus lysate with ab207755 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab207755 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: Mouse thymus lysate, 10 μg (Input).
Lane 2: ab207755 IP in Mouse thymus lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab207755 in Mouse thymus lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
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![Anti-CD4 antibody [EPR20122] (ab207755)](https://www.abcam.com/ps/products/207/ab207755/Images/ab207755-5-benefits-of-recombinant-antibodies.png)
Anti-CD4 antibody [EPR20122] (ab207755)
Protocols
Datasheets and documents
Certificate of Compliance
References (0)
ab207755 has not yet been referenced specifically in any publications.
