Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Lymphnode)
Permeabilization
No
Specification
Lymphnode
Blocking step
5% Normal Goat in 0.1% BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
Formaldehyde
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 23°C
Dilution
1/100
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Goat anti-rabbit, Alexa Fluor 568
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Additional data
Additional Notes
For antigen retrival: Citrate buffer, 30 min incubation at 96C
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. JIMENA TRILLO-TINOCO
Verified customer
Submitted Feb 13 2018