Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Thymus)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA
Permeabilization
Yes - H2O2
Specification
Thymus
Fixative
Formaldehyde
Other product details
Incubation time
36 minute(s) · Temperature: 37°C · Diluent: antibody dilution buffer
Dilution
1/250
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-Rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
The stained cells were also detected in the negative control tissue (no primary antibody treated).
Abcam response
Thank you for trying with a dog sample.We don't have this data yet.We look forward to feedback from other researchers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
Submitted Aug 16 2019