Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Syncerus caffer (African buffalo) Tissue sections (Lung and thoracic lymph nodes)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9
Permeabilization
No
Specification
Lung and thoracic lymph nodes
Blocking step
Peroxide Block as blocking agent for 5 minute(s) · Concentration: 30µg/mL
Fixative
Formaldehyde
Other product details
Incubation time
30 minute(s)
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
The CD4 marker did not work on my buffalo lung or lymph node tissue, however, it did work on the hum.an tonsil tissue we ran in parallel
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Samantha Goldswain
Verified customer
Submitted Mar 19 2020