Recombinant Anti-CD4 antibody [EPR7276] (ab133622)
![Western blot - Anti-CD4 antibody [EPR7276] (ab133622)](https://www.abcam.com/ps/products/133/ab133622/Images/ab133622-147506-anti-cd4-antibody-epr7276-western-blot.jpg "Western blot - Anti-CD4 antibody [EPR7276] (ab133622)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7276] to CD4
- Suitable for: WB, Flow Cyt
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-CD4 antibody [EPR7276]
See all CD4 primary antibodies -
Description
Rabbit monoclonal [EPR7276] to CD4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cytmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- Human fetal thymus and Human lymph node lysates, THP-1 cells
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7276 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133622 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
Abcam recommends using milk as the blocking agent. |
|
| Flow Cyt |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa). Abcam recommends using milk as the blocking agent. |
|
Flow Cyt
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
-
Function
Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. -
Sequence similarities
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsPalmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts. -
Cellular localization
Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum. - Information by UniProt
-
Database links
- Entrez Gene: 920 Human
- Omim: 186940 Human
- SwissProt: P01730 Human
- Unigene: 631659 Human
-
Alternative names
- CD 4 antibody
- CD4 (L3T4) antibody
- CD4 antibody
see all
Images
-
Western blot - Anti-CD4 antibody [EPR7276] (ab133622)All lanes : Anti-CD4 antibody [EPR7276] (ab133622) at 1/1000 dilution
Lane 1 : Human fetal thymus lysate
Lane 2 : Human lymph node lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 51 kDa
Observed band size: 51 kDa -
![Flow Cytometry - Anti-CD4 antibody [EPR7276] (ab133622)](https://www.abcam.com/ps/products/133/ab133622/Images/ab133622-147505-anti-cd4-antibody-epr7276-flow-cytometry.jpg)
Flow Cytometry - Anti-CD4 antibody [EPR7276] (ab133622)Flow Cytometrical analysis of THP-1 cells with ab133622 antibody at a dilution of 1/100 (red) or a rabbit IgG (negative) (green). -
![Anti-CD4 antibody [EPR7276] (ab133622)](https://www.abcam.com/ps/products/133/ab133622/Images/ab133622-3-benefits-of-recombinant-antibodies.png)
Anti-CD4 antibody [EPR7276] (ab133622)
Datasheets and documents
-
Datasheet download
References (0)
ab133622 has not yet been referenced specifically in any publications.
