Recombinant
RabMAb

Recombinant Anti-CD4 antibody [EPR7276] (ab133622)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7276] to CD4
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-CD4 antibody [EPR7276]
    See all CD4 primary antibodies

  • Description

    Rabbit monoclonal [EPR7276] to CD4

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment corresponding to the extracellular domain of Human CD4 (UniProt ID: P01730).

  • Positive control

    • Human fetal thymus and Human lymph node lysates, THP-1 cells

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab133622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).

Abcam recommends using milk as the blocking agent.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for IHC-P or IP.

    • Target

    • Function

      Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.

    • Sequence similarities

      Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.

    • Post-translational
      modifications

      Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.

    • Cellular localization

      Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
    • Information by UniProt

    • Database links

      • Alternative names

        • CD 4 antibody
        • CD4 (L3T4) antibody
        • CD4 antibody
        • CD4 antigen (p55) antibody
        • CD4 antigen antibody
        • CD4 molecule antibody
        • CD4 receptor antibody
        • CD4+ Lymphocyte deficiency, included antibody
        • CD4_HUMAN antibody
        • CD4mut antibody
        • L3T4 antibody
        • Leu3 antibody
        • Ly-4 antibody
        • Lymphocyte antigen CD4 antibody
        • MGC165891 antibody
        • OTTHUMP00000238897 antibody
        • p55 antibody
        • T cell antigen T4 antibody
        • T cell antigen T4/LEU3 antibody
        • T cell differentiation antigen L3T4 antibody
        • T cell OKT4 deficiency, included antibody
        • T cell surface antigen T4/Leu 3 antibody
        • T cell surface antigen T4/Leu3 antibody
        • T cell surface glycoprotein CD4 antibody
        • T-cell surface antigen T4/Leu-3 antibody
        • T-cell surface glycoprotein CD4 antibody
        • W3/25 antibody
        • W3/25 antigen antibody
        see all

      Images

      • Western blot - Anti-CD4 antibody [EPR7276] (ab133622)
        Western blot - Anti-CD4 antibody [EPR7276] (ab133622)
        All lanes : Anti-CD4 antibody [EPR7276] (ab133622) at 1/1000 dilution

        Lane 1 : Human fetal thymus lysate
        Lane 2 : Human lymph node lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 51 kDa
        Observed band size: 51 kDa

      • Flow Cytometry - Anti-CD4 antibody [EPR7276] (ab133622)
        Flow Cytometry - Anti-CD4 antibody [EPR7276] (ab133622)
        Flow Cytometrical analysis of THP-1 cells with ab133622 antibody at a dilution of 1/100 (red) or a rabbit IgG (negative) (green).

      References

      Publishing research using ab133622? Please let us know so that we can cite the reference in this datasheet.

      ab133622 has not yet been referenced specifically in any publications.

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