RecombinantRabMAb

Recombinant Anti-CD4 antibody [EPR7276] (ab133622)

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Western blot - Anti-CD4 antibody [EPR7276] (ab133622)
  • Flow Cytometry - Anti-CD4 antibody [EPR7276] (ab133622)
  • Anti-CD4 antibody [EPR7276] (ab133622)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7276] to CD4
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free

Overview

  • Product name

    Anti-CD4 antibody [EPR7276]
    See all CD4 primary antibodies

  • Description

    Rabbit monoclonal [EPR7276] to CD4

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal thymus and Human lymph node lysates, THP-1 cells

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).

Abcam recommends using milk as the blocking agent.
Flow Cyt
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).

Abcam recommends using milk as the blocking agent.
Flow Cyt
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Application notes
Is unsuitable for IHC-P or IP.

Target

  • Function

    Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.

  • Sequence similarities

    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • Post-translational
    modifications

    Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.

  • Cellular localization

    Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
  • Information by UniProt

  • Database links

    • Alternative names

      • CD 4 antibody
      • CD4 (L3T4) antibody
      • CD4 antibody
      • CD4 antigen (p55) antibody
      • CD4 antigen antibody
      • CD4 molecule antibody
      • CD4 receptor antibody
      • CD4+ Lymphocyte deficiency, included antibody
      • CD4_HUMAN antibody
      • CD4mut antibody
      • L3T4 antibody
      • Leu3 antibody
      • Ly-4 antibody
      • Lymphocyte antigen CD4 antibody
      • MGC165891 antibody
      • OTTHUMP00000238897 antibody
      • p55 antibody
      • T cell antigen T4 antibody
      • T cell antigen T4/LEU3 antibody
      • T cell differentiation antigen L3T4 antibody
      • T cell OKT4 deficiency, included antibody
      • T cell surface antigen T4/Leu 3 antibody
      • T cell surface antigen T4/Leu3 antibody
      • T cell surface glycoprotein CD4 antibody
      • T-cell surface antigen T4/Leu-3 antibody
      • T-cell surface glycoprotein CD4 antibody
      • W3/25 antibody
      • W3/25 antigen antibody
      see all

    Images

    • Western blot - Anti-CD4 antibody [EPR7276] (ab133622)
      Western blot - Anti-CD4 antibody [EPR7276] (ab133622)
      All lanes : Anti-CD4 antibody [EPR7276] (ab133622) at 1/1000 dilution

      Lane 1 : Human fetal thymus lysate
      Lane 2 : Human lymph node lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 51 kDa
      Observed band size: 51 kDa

    • Flow Cytometry - Anti-CD4 antibody [EPR7276] (ab133622)
      Flow Cytometry - Anti-CD4 antibody [EPR7276] (ab133622)
      Flow Cytometrical analysis of THP-1 cells with ab133622 antibody at a dilution of 1/100 (red) or a rabbit IgG (negative) (green).
    • Anti-CD4 antibody [EPR7276] (ab133622)
      Anti-CD4 antibody [EPR7276] (ab133622)

    References (0)

    Publishing research using ab133622? Please let us know so that we can cite the reference in this datasheet.

    ab133622 has not yet been referenced specifically in any publications.

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