Recombinant Anti-CD4 antibody [EPR7276] (ab133622)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-CD4 antibody [EPR7276]
See all CD4 primary antibodies -
Description
Rabbit monoclonal [EPR7276] to CD4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to the extracellular domain of Human CD4 (UniProt ID: P01730).
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Positive control
- Human fetal thymus and Human lymph node lysates, THP-1 cells
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7276 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab133622 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa). Abcam recommends using milk as the blocking agent. |
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| ICC/IF | 1/100 - 1/250. | |
| Flow Cyt | 1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. -
Sequence similarities
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsPalmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts. -
Cellular localization
Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 920 Human
- Omim: 186940 Human
- SwissProt: P01730 Human
- Unigene: 631659 Human
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Alternative names
- CD 4 antibody
- CD4 (L3T4) antibody
- CD4 antibody
see all
Images
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![Western blot - Anti-CD4 antibody [EPR7276] (ab133622)](https://www.abcam.com/ps/products/133/ab133622/Images/ab133622-147506-anti-cd4-antibody-epr7276-western-blot.jpg)
Western blot - Anti-CD4 antibody [EPR7276] (ab133622)All lanes : Anti-CD4 antibody [EPR7276] (ab133622) at 1/1000 dilution
Lane 1 : Human fetal thymus lysate
Lane 2 : Human lymph node lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 51 kDa
Observed band size: 51 kDa -
![Flow Cytometry - Anti-CD4 antibody [EPR7276] (ab133622)](https://www.abcam.com/ps/products/133/ab133622/Images/ab133622-147505-anti-cd4-antibody-epr7276-flow-cytometry.jpg)
Flow Cytometry - Anti-CD4 antibody [EPR7276] (ab133622)Flow Cytometrical analysis of THP-1 cells with ab133622 antibody at a dilution of 1/100 (red) or a rabbit IgG (negative) (green).
Protocols
Datasheets and documents
References
ab133622 has not yet been referenced specifically in any publications.
