Overview

  • Product name
    Anti-CD4 antibody [GK1.5]
    See all CD4 primary antibodies
  • Description
    Rat monoclonal [GK1.5] to CD4
  • Host species
    Rat
  • Specificity
    ab25475 recognises CD4/L3T4, Mr 52 kDa.
  • Tested applications
    Suitable for: Functional Studies, Flow Cyt, IP, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue/ cell preparation (Mouse) - cloned mouse CTL line V4.

  • General notes

    This antibody blocks helper T cell responses to MHC class II antigens, including cytolysis, proliferation, allogeneic B cell help, and release of lymphokines.

Properties

Applications

Our Abpromise guarantee covers the use of ab25475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration. PubMed: 6415170(In vivo and in vitro depletion of CD4+ cells; Inhibition of T helper function and Fractionation of CD4+ cells) Use at an assay dependent dilution. For details see: Kruisbeek, A. M., et al. 1991. In Current Protocols in Immunology. John Wiley & Sons, New York, p. 4.1.1. See also PubMed: 6415170. For details see: Kruisbeek, A. M., et al.
Flow Cyt Use at an assay dependent concentration.

ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. PubMed: 21345609

Target

  • Function
    Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
  • Sequence similarities
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
  • Cellular localization
    Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 4 antibody
    • CD4 (L3T4) antibody
    • CD4 antibody
    • CD4 antigen (p55) antibody
    • CD4 antigen antibody
    • CD4 molecule antibody
    • CD4 receptor antibody
    • CD4+ Lymphocyte deficiency, included antibody
    • CD4_HUMAN antibody
    • CD4mut antibody
    • L3T4 antibody
    • Leu3 antibody
    • Ly-4 antibody
    • Lymphocyte antigen CD4 antibody
    • MGC165891 antibody
    • OTTHUMP00000238897 antibody
    • p55 antibody
    • T cell antigen T4 antibody
    • T cell antigen T4/LEU3 antibody
    • T cell differentiation antigen L3T4 antibody
    • T cell OKT4 deficiency, included antibody
    • T cell surface antigen T4/Leu 3 antibody
    • T cell surface antigen T4/Leu3 antibody
    • T cell surface glycoprotein CD4 antibody
    • T-cell surface antigen T4/Leu-3 antibody
    • T-cell surface glycoprotein CD4 antibody
    • W3/25 antibody
    • W3/25 antigen antibody
    see all

Images

  • ab25475 staining CD4 (red) in mouse spleen tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 5% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in PBS) for 12 hours at 4°C. A Cy3®-conjugated Donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    Blue: DAPI.

    See Abreview

  • Flow Cytometry analysis of BALB/c mouse splenocytes labeling CD4 with ab25475 at 1 μg/106 cells dilution (purple). A Mouse Anti-Rat IgG2b-PE was used as the secondary antibody. Grey - Isotype Control, Rat IgG2b-UNLB followed by Mouse Anti-Rat IgG2b-PE.

References

This product has been referenced in:
  • Xu X  et al. Newcastle disease virus co-expressing interleukin 7 and interleukin 15 modified tumor cells as a vaccine for cancer immunotherapy. Cancer Sci 109:279-288 (2018). IHC-P ; Mouse . Read more (PubMed: 29224228) »
  • Nakajima Y  et al. Near-Infrared Fluorescence Imaging Directly Visualizes Lymphatic Drainage Pathways and Connections between Superficial and Deep Lymphatic Systems in the Mouse Hindlimb. Sci Rep 8:7078 (2018). Read more (PubMed: 29728629) »
See all 19 Publications for this product

Customer reviews and Q&As

1-10 of 10 Abreviews or Q&A

Question
Answer


I am very pleased to hear you would like to accept our offer and test ab25475 in IHC-P. This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview forIHC-P and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Liver, Spleen, Lung)
Antigen retrieval step
Heat mediated
Permeabilization
No
Specification
Liver, Spleen, Lung
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
no fixation

Abcam user community

Verified customer

Submitted Aug 15 2013

Answer

I can confirm that these two antibodies ab25478 and ab25475 are tested externally in functional studies.

There are publications where these clones have been tested in this application. I have attached thse for your review and hope this will be helpful.

The protocol referenced for CD4 antibody [GK1.5] can be found in the following book. Hathcock, K.S. 1991. In Current Protocols in Immunology John Wiley & Sons, New York, p. 3.4.1.

I hope this information will be helpful. Please do not hesitae to let me know if you have any questions or if I may assist you further.

Read More

Answer

Thank you for contacting us.

Our products of animal origin are derived from animals that have not given evidence of any disease, mentioned in list A of the Office of International des Epizooties (OIE). With that being said, our products are not specifically tested for pathogens; therefore, we are unable to make this guarantee.

All our products are for research use only and are not intended for diagnostic or therapeutic use.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Question
Answer

I am very pleased to hear you would like to accept our offer and test ab25475 inIHC-P. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forIHC-P and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Spleen)
Specification
Spleen
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jul 23 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (spleen)
Specification
spleen
Fixative
Formaldehyde
Permeabilization
Yes - 0.05%TritonX-100/PBS
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Dec 12 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (tumor mouse)
Specification
tumor mouse
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 20°C

Miss. Silke Vorwald

Verified customer

Submitted Sep 26 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (mouse skeletal muscle)
Specification
mouse skeletal muscle
Fixative
Acetone
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Feb 13 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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