APC Anti-CD4 antibody [GK1.5] (ab25497)
Key features and details
- APC Rat monoclonal [GK1.5] to CD4
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: APC. Ex: 645nm, Em: 660nm
- Isotype: IgG2b
Related conjugates and formulations
Overview
-
Product name
APC Anti-CD4 antibody [GK1.5]
See all CD4 primary antibodies -
Description
APC Rat monoclonal [GK1.5] to CD4 -
Host species
Rat -
Conjugation
APC. Ex: 645nm, Em: 660nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue/ cell preparation (Mouse) - cloned mouse CTL line V4.
-
General notes
This antibody blocks helper T cell responses to MHC class II antigens,including cytolysis, proliferation, allogeneic B cell help, and release of lymphokines.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C. It is important to note that this product is reported to be light sensitive. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.142% Dibasic monohydrogen sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin -
Concentration information loading...
-
Purity
Affinity purified -
Purification notes
Purified from TCS -
Primary antibody notes
This antibody blocks helper T cell responses to MHC class II antigens,including cytolysis, proliferation, allogeneic B cell help, and release of lymphokines. -
Clonality
Monoclonal -
Clone number
GK1.5 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25497 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use at an assay dependent concentration.
|
Notes |
---|
Flow Cyt
Use at an assay dependent concentration. |
Target
-
Function
Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. -
Sequence similarities
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsPalmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts. -
Cellular localization
Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum. - Information by UniProt
-
Database links
- Entrez Gene: 12504 Mouse
- SwissProt: P06332 Mouse
- Unigene: 2209 Mouse
-
Alternative names
- CD 4 antibody
- CD4 (L3T4) antibody
- CD4 antibody
see all
Datasheets and documents
-
Datasheet download
References (0)
ab25497 has not yet been referenced specifically in any publications.