Product nameAnti-CD4 antibody [GK1.5] (PerCP/Cy5.5®)
See all CD4 primary antibodies
DescriptionRat monoclonal [GK1.5] to CD4 (PerCP/Cy5.5®)
ConjugationPerCP/Cy5.5®. Ex: 482nm, Em: 690nm
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Mouse
Predicted to work with: Syrian hamster
The details of the immunogen for this antibody are not available.
- C57Bl/6 splenocytes.
Storage instructionsShipped at 4°C. Store at +4°C. Store In the Dark.
Storage bufferpH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 0.12% Monobasic dihydrogen sodium phosphate, 0.1% Gelatin, 0.87% Sodium chloride
Concentration information loading...
PurityProtein A purified
Purification notesab210265 was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Light chain typekappa
- Anti-CD4 antibody [GK1.5] (FITC) (ab218745)
- Anti-CD4 antibody [GK1.5] (CF405M) (ab237029)
- Anti-CD4 antibody [GK1.5] (violetFluor™ 450) (ab242009)
- Anti-CD4 antibody [GK1.5] (redFluor™ 710) (ab242010)
- Anti-CD4 antibody [GK1.5] (ab25475)
- Anti-CD4 antibody [GK1.5] (PE/Cy5®) (ab25476)
- Anti-CD4 antibody [GK1.5] (Allophycocyanin) (ab25497)
Our Abpromise guarantee covers the use of ab210265 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
Use 0.06 µg.
FunctionAccessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
Sequence similaritiesContains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
modificationsPalmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
Cellular localizationCell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
- Information by UniProt
- CD 4 antibody
- CD4 (L3T4) antibody
- CD4 antibody
ab210265 has not yet been referenced specifically in any publications.