Overview

  • Product name

    Anti-CD4 antibody [GK1.5] (violetFluor™ 450)
    See all CD4 primary antibodies
  • Description

    Rat monoclonal [GK1.5] to CD4 (violetFluor™ 450)
  • Host species

    Rat
  • Conjugation

    violetFluor™ 450
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Syrian hamster
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control

    • Flow Cyt: C57Bl/6 splenocytes.
  • General notes

    If used together, the GK1.5 antibody and an alternative antibody, anti-Mouse CD4 clone RM4-5, will compete for binding, i.e. RM4-5 is able to block GK1.5 binding to cells.  In contrast, the anti-Mouse CD4 clone RM4-4 does not block binding of the GK1.5 antibody to cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab242009 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

Target

  • Function

    Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
  • Sequence similarities

    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications

    Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
  • Cellular localization

    Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD 4 antibody
    • CD4 (L3T4) antibody
    • CD4 antibody
    • CD4 antigen (p55) antibody
    • CD4 antigen antibody
    • CD4 molecule antibody
    • CD4 receptor antibody
    • CD4+ Lymphocyte deficiency, included antibody
    • CD4_HUMAN antibody
    • CD4mut antibody
    • L3T4 antibody
    • Leu3 antibody
    • Ly-4 antibody
    • Lymphocyte antigen CD4 antibody
    • MGC165891 antibody
    • OTTHUMP00000238897 antibody
    • p55 antibody
    • T cell antigen T4 antibody
    • T cell antigen T4/LEU3 antibody
    • T cell differentiation antigen L3T4 antibody
    • T cell OKT4 deficiency, included antibody
    • T cell surface antigen T4/Leu 3 antibody
    • T cell surface antigen T4/Leu3 antibody
    • T cell surface glycoprotein CD4 antibody
    • T-cell surface antigen T4/Leu-3 antibody
    • T-cell surface glycoprotein CD4 antibody
    • W3/25 antibody
    • W3/25 antigen antibody
    see all

Images

  • Flow cytometric analysis of C57Bl/6 splenocytes stained for CD8CD4 with 0.25 µg ab242009 (solid line) or 0.25 µg violetFluor™ 450 Rat IgG2b isotype control (dashed line).

References

ab242009 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab242009.
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