violetFluor™ 450 Anti-CD4 antibody [GK1.5] (ab242009)
Key features and details
- violetFluor™ 450 Rat monoclonal [GK1.5] to CD4
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: violetFluor™ 450. Ex: 405nm, Em: 450nm
- Isotype: IgG2b
Related conjugates and formulations
Overview
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Product name
violetFluor™ 450 Anti-CD4 antibody [GK1.5]
See all CD4 primary antibodies -
Description
violetFluor™ 450 Rat monoclonal [GK1.5] to CD4 -
Host species
Rat -
Conjugation
violetFluor™ 450. Ex: 405nm, Em: 450nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Syrian hamster -
Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- Flow Cyt: C57Bl/6 splenocytes.
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General notes
If used together, the GK1.5 antibody and an alternative antibody, anti-Mouse CD4 clone RM4-5, will compete for binding, i.e. RM4-5 is able to block GK1.5 binding to cells. In contrast, the anti-Mouse CD4 clone RM4-4 does not block binding of the GK1.5 antibody to cells.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.12% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. -
Clonality
Monoclonal -
Clone number
GK1.5 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab242009 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use at an assay dependent concentration.
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Notes |
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Flow Cyt
Use at an assay dependent concentration. |
Target
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Function
Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. -
Sequence similarities
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsPalmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts. -
Cellular localization
Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 12504 Mouse
- SwissProt: P06332 Mouse
- Unigene: 2209 Mouse
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Alternative names
- CD 4 antibody
- CD4 (L3T4) antibody
- CD4 antibody
see all
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab242009 has not yet been referenced specifically in any publications.