Key features and details
- Mouse monoclonal [MEM-241] to CD4 (APC)
- Suitable for: Flow Cyt, WB
- Reacts with: Human
- Conjugation: APC. Ex: 645nm, Em: 660nm
- Isotype: IgG1
Product nameAnti-CD4 antibody [MEM-241] (APC)
See all CD4 primary antibodies
DescriptionMouse monoclonal [MEM-241] to CD4 (APC)
ConjugationAPC. Ex: 645nm, Em: 660nm
Tested applicationsSuitable for: Flow Cyt, WBmore details
Species reactivityReacts with: Human
Fusion protein (Human).
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Constituents: PBS, BSA
Concentration information loading...
PurityProtein A purified
- Anti-CD4 antibody [MEM-241] (FITC) (ab18281)
- Anti-CD4 antibody [MEM-241] (PE) (ab18282)
- Anti-CD4 antibody [MEM241] (APC/Cy7®) (ab233298)
- Anti-CD4 antibody [MEM241] (PE/Cy7®) (ab233660)
- Anti-CD4 antibody [MEM-241] (Alexa Fluor® 700) (ab270671)
- Anti-CD4 antibody [MEM-241] (Biotin) (ab28069)
- Anti-CD4 antibody [MEM-241] (PerCP) (ab65951)
Our Abpromise guarantee covers the use of ab18280 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab37391 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|WB||Use at an assay dependent concentration. Detects a band of approximately 59 kDa.|
FunctionAccessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
Sequence similaritiesContains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
modificationsPalmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
Cellular localizationCell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
- Information by UniProt
- CD 4 antibody
- CD4 (L3T4) antibody
- CD4 antibody
Surface staining of human peripheral blood cells with anti-human CD4 (MEM-241) APC.
Overlay histogram showing peripheral blood lymphocytes stained with ab18280 (red line). The cells were incubated with the antibody (ab18280, 1/20 dilution) for 30 min at 4ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) with secondary antibody DyLight® 649 goat anti-mouse IgG (H&L) (ab96882) at 1/200 dilution for 30 min at 4ºC. Acquisition of >5,000 events was performed gating on peripheral blood lymphocytes.
ab18280 has not yet been referenced specifically in any publications.