Key features and details
- Mouse monoclonal [MEM-241] to CD4 (FITC)
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Product nameAnti-CD4 antibody [MEM-241] (FITC)
See all CD4 primary antibodies
DescriptionMouse monoclonal [MEM-241] to CD4 (FITC)
ConjugationFITC. Ex: 493nm, Em: 528nm
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Human
Fusion protein (Human).
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Constituents: PBS, BSA
Concentration information loading...
PurityProtein G purified
- Anti-CD4 antibody [MEM-241] (APC) (ab18280)
- Anti-CD4 antibody [MEM-241] (PE) (ab18282)
- Anti-CD4 antibody [MEM241] (APC/Cy7®) (ab233298)
- Anti-CD4 antibody [MEM241] (PE/Cy7®) (ab233660)
- Anti-CD4 antibody [MEM-241] (Alexa Fluor® 700) (ab270671)
- Anti-CD4 antibody [MEM-241] (Biotin) (ab28069)
- Anti-CD4 antibody [MEM-241] (PerCP) (ab65951)
Our Abpromise guarantee covers the use of ab18281 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 4.15µl for 106 cells.
ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionAccessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
Sequence similaritiesContains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
modificationsPalmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
Cellular localizationCell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
- Information by UniProt
- CD 4 antibody
- CD4 (L3T4) antibody
- CD4 antibody
Overlay histogram showing peripheral blood lymphocytes stained with ab18281 (red line). The cells incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18281, 0.5 µg/1x106 cells) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 FITC (2 µg/1x106 cells ) for 30 min at 22°C. Acquisition of >5,000 events was performed.
ab18281 has not yet been referenced specifically in any publications.