FITC Anti-CD4 antibody [OKT4] (ab210322)
Key features and details
- FITC Mouse monoclonal [OKT4] to CD4
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2b
Related conjugates and formulations
Overview
-
Product name
FITC Anti-CD4 antibody [OKT4]
See all CD4 primary antibodies -
Description
FITC Mouse monoclonal [OKT4] to CD4 -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Cynomolgus monkey, Rhesus monkey -
Immunogen
The details of the immunogen for this antibody are not available.
-
Positive control
- Human peripheral blood lymphocytes
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.12% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin -
Concentration information loading...
-
Purity
Protein A purified -
Purification notes
ab210322 was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. -
Clonality
Monoclonal -
Clone number
OKT4 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab210322 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use 5µl for 105-8 cells.
The antibody has been diluted for use at 5 µl (0.25 µg) per test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 µl. |
Notes |
---|
Flow Cyt
Use 5µl for 105-8 cells. The antibody has been diluted for use at 5 µl (0.25 µg) per test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 µl. |
Target
-
Function
Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. -
Sequence similarities
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsPalmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts. -
Cellular localization
Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum. - Information by UniProt
-
Database links
- Entrez Gene: 450124 Chimpanzee
- Entrez Gene: 101864991 Cynomolgus monkey
- Entrez Gene: 920 Human
- Entrez Gene: 713807 Rhesus monkey
- Omim: 186940 Human
- SwissProt: P16004 Chimpanzee
- SwissProt: P79185 Cynomolgus monkey
- SwissProt: P01730 Human
see all -
Alternative names
- CD 4 antibody
- CD4 (L3T4) antibody
- CD4 antibody
see all
Images
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab210322 has not yet been referenced specifically in any publications.