Overview

  • Product name

    Anti-CD4 antibody [OKT4] (PerCP/Cy5.5®)
    See all CD4 primary antibodies
  • Description

    Mouse monoclonal [OKT4] to CD4 (PerCP/Cy5.5®)
  • Host species

    Mouse
  • Conjugation

    PerCP/Cy5.5®. Ex: 482nm, Em: 690nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Cynomolgus monkey, Rhesus monkey
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control

    • Human peripheral blood lymphocytes.
  • General notes

    This antibody recognizes a different epitope, and thus does not block binding of, the alternative Anti-Human CD4 antibody clone RPA-T4.

Properties

Applications

Our Abpromise guarantee covers the use of ab210324 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 5µl for 105-8 cells.

The antibody has been diluted for use at 5 µl (0.25 μg) per test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 µl.

Target

  • Function

    Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
  • Sequence similarities

    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications

    Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
  • Cellular localization

    Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
  • Information by UniProt
  • Database links

    see all
  • Alternative names

    • CD 4 antibody
    • CD4 (L3T4) antibody
    • CD4 antibody
    • CD4 antigen (p55) antibody
    • CD4 antigen antibody
    • CD4 molecule antibody
    • CD4 receptor antibody
    • CD4+ Lymphocyte deficiency, included antibody
    • CD4_HUMAN antibody
    • CD4mut antibody
    • L3T4 antibody
    • Leu3 antibody
    • Ly-4 antibody
    • Lymphocyte antigen CD4 antibody
    • MGC165891 antibody
    • OTTHUMP00000238897 antibody
    • p55 antibody
    • T cell antigen T4 antibody
    • T cell antigen T4/LEU3 antibody
    • T cell differentiation antigen L3T4 antibody
    • T cell OKT4 deficiency, included antibody
    • T cell surface antigen T4/Leu 3 antibody
    • T cell surface antigen T4/Leu3 antibody
    • T cell surface glycoprotein CD4 antibody
    • T-cell surface antigen T4/Leu-3 antibody
    • T-cell surface glycoprotein CD4 antibody
    • W3/25 antibody
    • W3/25 antigen antibody
    see all

Images

  • Flow cytometric analysis of Human peripheral blood lymphocytes labeling CD4 with 5 μl (0.25 μg) ab210324 (solid line), compared with 0.25 μg of PerCP/Cy5.5® Mouse IgG2b isotype control (dashed line).

References

This product has been referenced in:

  • Hu Y  et al. Effect of TGF-ß1 on blood CD4+CD25high regulatory T cell proliferation and Foxp3 expression during non-small cell lung cancer blood metastasis. Exp Ther Med 16:1403-1410 (2018). Read more (PubMed: 30112067) »
See 1 Publication for this product

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