Anti-CD4 antibody [OX-35] (ab33775)
Key features and details
- Mouse monoclonal [OX-35] to CD4
- Suitable for: Flow Cyt
- Reacts with: Rat
- Isotype: IgG2a
Related conjugates and formulations
Overview
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Product name
Anti-CD4 antibody [OX-35]
See all CD4 primary antibodies -
Description
Mouse monoclonal [OX-35] to CD4 -
Host species
Mouse -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Tissue, cells or virus corresponding to Rat CD4. MLR generated rat T helper lymphocytes
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Epitope
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Purified by Protein A affinity chromatography -
Clonality
Monoclonal -
Clone number
OX-35 -
Myeloma
NS1 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab33775 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use a concentration of 1 - 4 µg/ml.
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Notes |
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Flow Cyt
Use a concentration of 1 - 4 µg/ml. |
Target
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Function
Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. -
Sequence similarities
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsPalmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts. -
Cellular localization
Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 24932 Rat
- SwissProt: P05540 Rat
- Unigene: 10748 Rat
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Alternative names
- CD 4 antibody
- CD4 (L3T4) antibody
- CD4 antibody
see all
Images
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Flow cytometric analysis using surface staining of rat splenocyte suspension. Rat splenocytes are stained using 1.6µg/ml ab33775 anti-rat CD4, GAM PE, (red) and are separated from REH cells stained with 1.6µg/ml mouse isotype control purified antibody
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Surface staining flow cytometric pattern of rat splenocytes stained with 1.6µg/ml ab33775 anti-rat CD4 purified antibody, GAM PE
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab33775 has been referenced in 8 publications.
- Lane EL et al. Spontaneous Graft-Induced Dyskinesias Are Independent of 5-HT Neurons and Levodopa Priming in a Model of Parkinson's Disease. Mov Disord 37:613-619 (2022). PubMed: 34766658
- Vainio SK et al. Dimethyl fumarate decreases short-term but not long-term inflammation in a focal EAE model of neuroinflammation. EJNMMI Res 12:6 (2022). PubMed: 35107664
- Heuer A et al. hESC-derived neural progenitors prevent xenograft rejection through neonatal desensitisation. Exp Neurol 282:78-85 (2016). IHC ; Rat . PubMed: 27235932
- Ellison SM et al. Dose-dependent Neuroprotection of VEGF165 in Huntington's Disease Striatum. Mol Ther 21:1862-1875 (2013). PubMed: 23799534
- Watanabe R et al. Inhibition of NF-?B activation by a novel IKK inhibitor reduces the severity of experimental autoimmune myocarditis via suppression of T-cell activation. Am J Physiol Heart Circ Physiol 305:H1761-71 (2013). PubMed: 24097428
- Thampi P et al. Pathological effects of processed bovine pericardial scaffolds--a comparative in vivo evaluation. Artif Organs 37:600-5 (2013). IHC ; Rat . PubMed: 23452255
- Randall KJ & Pearse G A dual-label technique for the immunohistochemical demonstration of T-lymphocyte subsets in formalin-fixed, paraffin-embedded rat lymphoid tissue. Toxicol Pathol 36:795-804 (2008). PubMed: 18776164
- Pohlers D et al. Differential clinical efficacy of anti-CD4 monoclonal antibodies in rat adjuvant arthritis is paralleled by differential influence on NF-kappaB binding activity and TNF-alpha secretion of T cells. Arthritis Res 4:184-9 (2002). PubMed: 12010568