Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.09% Sodium Azide
Constituents: PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab33775 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent concentration.|
|Flow Cyt||1/10 - 1/50.
Use 10µl of the suggested working dilution to label 106 cells in 100µl.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|IHC-Fr||Use at an assay dependent concentration.|
FunctionAccessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
Sequence similaritiesContains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
modificationsPalmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
Cellular localizationCell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
- Information by UniProt
- CD 4 antibody
- CD4 (L3T4) antibody
- CD4 antibody
This product has been referenced in:
- Heuer A et al. hESC-derived neural progenitors prevent xenograft rejection through neonatal desensitisation. Exp Neurol 282:78-85 (2016). IHC ; Rat . Read more (PubMed: 27235932) »
- Ellison SM et al. Dose-dependent Neuroprotection of VEGF165 in Huntington's Disease Striatum. Mol Ther 21:1862-1875 (2013). Read more (PubMed: 23799534) »