Overview

  • Product name
    Anti-CD4 antibody [OX-35]
    See all CD4 primary antibodies
  • Description
    Mouse monoclonal [OX-35] to CD4
  • Host species
    Mouse
  • Tested applications
    Suitable for: ICC, Flow Cyt, IHC-Frmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    MLR generated rat T helper lymphocytes.

  • Epitope
    This antibody recognises a different epitope on the CD4 molecule than the W3/25 monoclonal (eg ab33779 and ab33783).

Properties

Applications

Our Abpromise guarantee covers the use of ab33775 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
Flow Cyt 1/10 - 1/50.

Use 10µl of the suggested working dilution to label 106 cells in 100µl.

 

 

 

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

IHC-Fr Use at an assay dependent concentration.

Target

  • Function
    Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
  • Sequence similarities
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
  • Cellular localization
    Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 4 antibody
    • CD4 (L3T4) antibody
    • CD4 antibody
    • CD4 antigen (p55) antibody
    • CD4 antigen antibody
    • CD4 molecule antibody
    • CD4 receptor antibody
    • CD4+ Lymphocyte deficiency, included antibody
    • CD4_HUMAN antibody
    • CD4mut antibody
    • L3T4 antibody
    • Leu3 antibody
    • Ly-4 antibody
    • Lymphocyte antigen CD4 antibody
    • MGC165891 antibody
    • OTTHUMP00000238897 antibody
    • p55 antibody
    • T cell antigen T4 antibody
    • T cell antigen T4/LEU3 antibody
    • T cell differentiation antigen L3T4 antibody
    • T cell OKT4 deficiency, included antibody
    • T cell surface antigen T4/Leu 3 antibody
    • T cell surface antigen T4/Leu3 antibody
    • T cell surface glycoprotein CD4 antibody
    • T-cell surface antigen T4/Leu-3 antibody
    • T-cell surface glycoprotein CD4 antibody
    • W3/25 antibody
    • W3/25 antigen antibody
    see all

References

This product has been referenced in:
  • Heuer A  et al. hESC-derived neural progenitors prevent xenograft rejection through neonatal desensitisation. Exp Neurol 282:78-85 (2016). IHC ; Rat . Read more (PubMed: 27235932) »
  • Ellison SM  et al. Dose-dependent Neuroprotection of VEGF165 in Huntington's Disease Striatum. Mol Ther 21:1862-1875 (2013). Read more (PubMed: 23799534) »
See all 5 Publications for this product

Customer reviews and Q&As

Answer

Thank you for contacting us. ab31630, can be used in IHC-P ab8536, can be used in IHC-P ab65865; IHC-P is yet not tested we are unable to confirm it. Can we suggest alternatives as ab34364, ab87951 and ab101500? ab33775; IHC-P is not a tested application we are unable to confirm its use. We suggest using alternative as ab846, ab51312 ab33854; IHC-P again is not a tested application for this ab. We can suggest using ab9496, ab59754 In case you are interested in buying ab65865, ab33775 and ab33854 only I can then offer you three Abreview discount codes which will give you next three antibodies free of charge. Please be advised that in some cases heat damages the antigenic epitope we therefore always recommend our customers optimizing the heat mediated antigen retrieval step. Let me know if you are interested in my offer; I will then send you the discount codes. The alternatives are recommended based on their use in IHC-P so I would also suggest carefully checking the datasheet particularly immunogen and species in case you plan to use these antibodies. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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