Product nameCD4 Antibody Panel (EPR6855, SP35, BL-155-1c11)
See all CD4 kits
Species reactivityReacts with: Human
CD4 Antibody Panel ab252199 contains multiple trial-sized versions of anti-human antibody clones against CD4, specifically selected for high performance in IHC. This panel contains 3 recombinant rabbit monoclonal antibodies against human CD4. They are provided in trial sizes to allow you to easily evaluate which is the best CD4 antibody for your IHC research assay needs.
For guidelines on how to use each antibody within the panel, please consult the individual datasheet for each antibody.
- Rabbit monoclonal [BL-155-1C11] to CD4 (20 µL) ab243872
- Rabbit monoclonal [EPR6855] to CD4 (20 µL) ab133616
- Rabbit monoclonal [SP35] to CD4 (20 µL) ab213215
Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Carrier-free formulations of our recombinant antibodies are available and ready to use for multiplex IHC analysis including Imaging Mass CytometryTM. Please refer to the ‘Associated products’ section below.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 kit ab243872 - Anti-CD4 antibody [BL-155-1C11] 2 x 10µl Anti-CD4 antibody [EPR6855] (ab133616) 2 x 10µl ab213215 - Anti-CD4 antibody [SP35] 2 x 10µl
FunctionAccessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
Sequence similaritiesContains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
modificationsPalmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
Cellular localizationCell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
- Information by UniProt
- CD 4
- CD4 (L3T4)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling CD4 with ab213215 at 1/50 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD4 with purified ab133616 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Formalin-fixed, paraffin-embedded human appendix tissue stained for CD4 using ab243872 at 1/200 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab252199 has not yet been referenced specifically in any publications.