Product nameAnti-CD4 antibody [RM4-5] (PE/Cy7 ®)
See all CD4 primary antibodies
DescriptionRat monoclonal [RM4-5] to CD4 (PE/Cy7 ®)
ConjugationPE/Cy7 ®. Ex: 496nm, Em: 774nm
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Mouse
The details of the immunogen for this antibody are not available.
- C57Bl/6 splenocytes.
The RM4-5 antibody is widely used as a phenotypic marker for CD4 expression.
Storage instructionsShipped at 4°C. Store at +4°C. Store In the Dark.
Storage bufferpH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.12% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin
Concentration information loading...
Purification notesab210351 was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Light chain typekappa
- Anti-CD4 antibody [RM4-5] (Allophycocyanin) (ab210347)
- Anti-CD4 antibody [RM4-5] (Allophycocyanin/Cy7 ®) (ab210348)
- Anti-CD4 antibody [RM4-5] (FITC) (ab210349)
- Anti-CD4 antibody [RM4-5] (Phycoerythrin) (ab210350)
- Anti-CD4 antibody [RM4-5] (violetFluor™ 450) (ab241097)
- Anti-CD4 antibody [RM4-5] (redFluor™ 710) (ab242011)
Our Abpromise guarantee covers the use of ab210351 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
Use 0.06 µg per test
FunctionAccessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
Sequence similaritiesContains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
modificationsPalmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
Cellular localizationCell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
- Information by UniProt
- CD 4 antibody
- CD4 (L3T4) antibody
- CD4 antibody
ab210351 has not yet been referenced specifically in any publications.