Overview

  • Product name

    Anti-CD4 antibody [SP35]
    See all CD4 primary antibodies
  • Description

    Rabbit monoclonal [SP35] to CD4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Synthetic peptide within Human CD4 aa 50-150 (internal sequence). The exact sequence is proprietary.
    Database link: P01730

  • Positive control

    • Flow Cyt: THP-1 and Jurkat cells. IHC-P: Human Hodgkin's lymphoma and tonsil tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab213215 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50.

Deparaffinize slides using xylene or xylene alternative and graded alcohols. Boil tissue section in EDTA buffer for 10 min followed by cooling at room temperature for 20 min.  Incubate with primary antibody for 30 minutes at room temperature. Slides must be washed in between steps.  Rinse slides with PBS/0.05% Tween. 

Flow Cyt 1/20 - 1/100.

Incubate with primary antibody for 30 minutes at 4°C.

Target

  • Function

    Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
  • Sequence similarities

    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications

    Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
  • Cellular localization

    Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD 4 antibody
    • CD4 (L3T4) antibody
    • CD4 antibody
    • CD4 antigen (p55) antibody
    • CD4 antigen antibody
    • CD4 molecule antibody
    • CD4 receptor antibody
    • CD4+ Lymphocyte deficiency, included antibody
    • CD4_HUMAN antibody
    • CD4mut antibody
    • L3T4 antibody
    • Leu3 antibody
    • Ly-4 antibody
    • Lymphocyte antigen CD4 antibody
    • MGC165891 antibody
    • OTTHUMP00000238897 antibody
    • p55 antibody
    • T cell antigen T4 antibody
    • T cell antigen T4/LEU3 antibody
    • T cell differentiation antigen L3T4 antibody
    • T cell OKT4 deficiency, included antibody
    • T cell surface antigen T4/Leu 3 antibody
    • T cell surface antigen T4/Leu3 antibody
    • T cell surface glycoprotein CD4 antibody
    • T-cell surface antigen T4/Leu-3 antibody
    • T-cell surface glycoprotein CD4 antibody
    • W3/25 antibody
    • W3/25 antigen antibody
    see all

Images

  • Flow cytometry analysis of THP-1 (human acute monocytic leukemia) labeling CD4 with purified ab213215 at 1/20 dilution (7.75 µg/ml) (red).

    Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlableled control - Unlabeled cells (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human Hodgkin lymphoma tissue sections labeling CD4 with ab213215 at 1/50 dilution (3.1 µg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human Hodgkin's lymphoma, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab213215 for 30 mins at room temperature.

  • Foreskin epidermis at 10× magnification stained with CCR5 (pseudocolored green), CD4 (pseudocolored red; ab213215) and SYTOX Orange (pseudocolored blue) for nuclear identification. All fields from ∼26.5 mm2 sections were used for analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling CD4 with ab213215 at 1/50 dilution (3.1 µg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab213215 for 30 mins at room temperature.

  • Flow cytometric analysis of Jurkat (Human T cell leukemia cell line from peripheral blood) cells labeling CD4 with ab213215 at 1/100 dilution (green), compared with a rabbit IgG negative control (blue).

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling CD4 with ab213215 at 1/50 dilution.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling CD4 with ab213215 at 1/50 dilution.

References

This product has been referenced in:

  • Scimeca M  et al. Programmed death ligand 1 expression in prostate cancer cells is associated with deep changes of the tumor inflammatory infiltrate composition. Urol Oncol 37:297.e19-297.e31 (2019). Read more (PubMed: 30827759) »
  • Oishi N  et al. Cutaneous lesions of angioimmunoblastic T-cell lymphoma: Clinical, pathological, and immunophenotypic features. J Cutan Pathol N/A:N/A (2019). Read more (PubMed: 30980412) »
See all 7 Publications for this product

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