Recombinant
RabMAb

Recombinant Anti-CD4 antibody [SP35], prediluted (ab101530)

Overview

  • Product name

    Anti-CD4 antibody [SP35], prediluted
    See all CD4 primary antibodies
  • Description

    Rabbit monoclonal [SP35] to CD4, prediluted
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Pig
  • Immunogen

    Synthetic peptide. This information is considered to be commercially sensitive.

  • Positive control

    • Human Tonsil, Human T cell lymphoma

Properties

Applications

Our Abpromise guarantee covers the use of ab101530 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P Use at an assay dependent concentration.

Antigen retrieval is recommended: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

Target

  • Function

    Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
  • Sequence similarities

    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications

    Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
  • Cellular localization

    Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD 4 antibody
    • CD4 (L3T4) antibody
    • CD4 antibody
    • CD4 antigen (p55) antibody
    • CD4 antigen antibody
    • CD4 molecule antibody
    • CD4 receptor antibody
    • CD4+ Lymphocyte deficiency, included antibody
    • CD4_HUMAN antibody
    • CD4mut antibody
    • L3T4 antibody
    • Leu3 antibody
    • Ly-4 antibody
    • Lymphocyte antigen CD4 antibody
    • MGC165891 antibody
    • OTTHUMP00000238897 antibody
    • p55 antibody
    • T cell antigen T4 antibody
    • T cell antigen T4/LEU3 antibody
    • T cell differentiation antigen L3T4 antibody
    • T cell OKT4 deficiency, included antibody
    • T cell surface antigen T4/Leu 3 antibody
    • T cell surface antigen T4/Leu3 antibody
    • T cell surface glycoprotein CD4 antibody
    • T-cell surface antigen T4/Leu-3 antibody
    • T-cell surface glycoprotein CD4 antibody
    • W3/25 antibody
    • W3/25 antigen antibody
    see all

Images

  • Flow cytometric analysis of rabbit anti-CD4 (SP35) antibody, prediluted, ab101530 in Jurkats cells (green) compared to negative control of rabbit IgG (blue)

  • Staining of Human CD4 in a Formalin/PFA-fixed paraffin-embedded section of Human T cell lymphoma using ab101530 at a dilution of 1/1.

References

This product has been referenced in:

  • Ravanidis S  et al. Subcutaneous Transplantation of Neural Precursor Cells in Experimental Autoimmune Encephalomyelitis Reduces Chemotactic Signals in the Central Nervous System. Stem Cells Transl Med 4:1450-62 (2015). Read more (PubMed: 26511651) »
  • Wick DA  et al. Surveillance of the tumor mutanome by T cells during progression from primary to recurrent ovarian cancer. Clin Cancer Res 20:1125-34 (2014). Read more (PubMed: 24323902) »
See all 2 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 40% · Temperature: 20°C
Sample
Human Tissue sections (human tonsil)
Specification
human tonsil
Permeabilization
Yes - saponin
Fixative
Paraformaldehyde

Mr. Jan Misiak

Verified customer

Submitted May 13 2014

Answer

Thank you for your enquiry.

I have checked these product numbers. I am sorry to confirm that ab4098 is discontinued and apologize for the inconvenience.

ab4098 is an antibody for detection of Nucleophosmin. However, ab101530 is an antibody for detection of CD4, which is a different protein. I would appreciate if you could you confirm these are the correctcatalog numbers so I can provide you withright information?

Please let me know which application and species the customer will be using, and for detection of which protein, and I will be pleased to find a suitable product for them.

Many thanks for your cooperation. I look forward to hearing from you.

Read More

Answer

Thank you very much for your phone call.

The best antibodies we can recommend are as follows. Please note that the recommendations are based on immunogen sequence only we currently do not hold any rabbit specific data.

Anti CD3 : ab16669; I can provide Abreview image discount code and will also provide Abpromise guarantee on use for this ab.

Anti FOXP3: ab20034, ab22510 are best antibodies we can offer. Eligible for new species Abreview discount code however not eligible for refund in case it does not work.

Anti CD4: ab61131, ab101530 and ab108329, are the best antibodies we can offer. Eligible for new species Abreview discount code however not eligible for refund in case it does not work.

anti CD8: ab108343, ab123630 and ab86733, are the best antibodies we can offer. Eligible for new species Abreview discount code however not eligible for refund in case it does not work.

Anti CD1A : ab76531, ab108309 and ab123133 are the best antibodies we can offer. Eligible for new species Abreview discount code however not eligible for refund in case it does not work.

Please also note that most of these antibodies are raised in Rabbit so there will be high back ground staining. Our recommendations to reduce the back ground staining are

- Use preadsorbed anti body e.g. ab7132, ab7083, ab

- Use (Fab)2 fragments antibodies e.g. ab6112, ab102296

- Incubate sections with affinity pure Fab fragment anti rabbit antibody before applying primary antibody

Please check the full details by clicking the links;

https://www.abcam.com/index.html?pageconfig=resource&rid=12722
https://www.abcam.com/index.html?pageconfig=resource&rid=12825
https://www.abcam.com/index.html?pageconfig=resource&rid=11465

The abreview discount code details can be found by clicking following link;

https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount

Could you please select the antibodies you are interested in, I will then be happy in creating a discount code for each one of these.

I will look forward to hearing from you soon.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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