Anti-CD40 antibody [3/23] (ab22469)
- Datasheet
- References (4)
- Protocols
Overview
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Product name
Anti-CD40 antibody [3/23]
See all CD40 primary antibodies -
Description
Rat monoclonal [3/23] to CD40 -
Host species
Rat -
Specificity
Does not react with normal mouse Ig or with human IgG1 and will stain most mature mouse B cells. Does not cross react with mouse T cells. ab22469 is a powerful activator of normal B cells especially in the presence of IL4. -
Tested applications
Suitable for: ELISA, Flow Cyt, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Extracellular Domain of Mouse CD40 and the Fc portion of Human IgG1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
3/23 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab22469 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | Use at an assay dependent concentration. | |
| Flow Cyt | Use 10µl for 106 cells. ab37371 - Rat polyclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| IHC-Fr | Use at an assay dependent concentration. |
Target
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Function
Receptor for TNFSF5/CD40LG. -
Tissue specificity
B-cells and in primary carcinomas. -
Involvement in disease
Defects in CD40 are the cause of hyper-IgM immunodeficiency syndrome type 3 (HIGM3) [MIM:606843]; also known as hyper-IgM syndrome 3. HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation. -
Sequence similarities
Contains 4 TNFR-Cys repeats. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 958 Human
- Entrez Gene: 21939 Mouse
- Omim: 109535 Human
- SwissProt: P25942 Human
- SwissProt: P27512 Mouse
- Unigene: 472860 Human
- Unigene: 271833 Mouse
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Alternative names
- AI326936 antibody
- B cell associated molecule CD40 antibody
- B cell surface antigen CD40 antibody
see all
Images
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![Flow Cytometry - Anti-CD40 antibody [3/23] (ab22469)](https://www.abcam.com/ps/products/22/ab22469/Images/ab22469-31849-anti-cd40-antibody-3-23-flow-cytometry.jpg)
Flow Cytometry - Anti-CD40 antibody [3/23] (ab22469)ab22469 staining CD40 in Mouse Spleen cells by flow cytometric analysis.
References
This product has been referenced in:
- Benchetrit H et al. Direct Induction of the Three Pre-implantation Blastocyst Cell Types from Fibroblasts. Cell Stem Cell 24:983-994.e7 (2019). Read more (PubMed: 31031139) »
- Carriba P & Davies AM CD40 is a major regulator of dendrite growth from developing excitatory and inhibitory neurons. Elife 6:N/A (2017). Read more (PubMed: 29111976) »
