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Immunology Adaptive Immunity B Cells CD
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Anti-CD40 antibody [3/23] (ab22469)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (4)

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Flow Cytometry - Anti-CD40 antibody [3/23] (ab22469)

    Key features and details

    • Rat monoclonal [3/23] to CD40
    • Suitable for: Flow Cyt
    • Reacts with: Mouse
    • Isotype: IgG

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    Overview

    • Product name

      Anti-CD40 antibody [3/23]
      See all CD40 primary antibodies
    • Description

      Rat monoclonal [3/23] to CD40
    • Host species

      Rat
    • Specificity

      Does not react with normal mouse Ig or with human IgG1 and will stain most mature mouse B cells. Does not cross react with mouse T cells. ab22469 is a powerful activator of normal B cells especially in the presence of IL4.
    • Tested Applications & Species

      Application Species
      Flow Cyt
      Mouse
      See all applications and species data
    • Immunogen

      Extracellular Domain of Mouse CD40 and the Fc portion of Human IgG1.

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
    • Storage buffer

      pH: 7.40
      Preservative: 0.1% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • Purity

      Protein G purified
    • Clonality

      Monoclonal
    • Clone number

      3/23
    • Isotype

      IgG
    • Research areas

      • Immunology
      • Adaptive Immunity
      • B Cells
      • CD
      • Cardiovascular
      • Atherosclerosis
      • Vascular Inflammation
      • Innate and adaptive immunity
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • B Lymphocytic Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Myeloid
      • Dendritic Cell Lineage

    Associated products

    • Alternative Versions

      • FITC Anti-CD40 antibody [3/23] (ab22470)
    • Compatible Secondaries

      • Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
      • Goat Anti-Rat IgG H&L (HRP) (ab205720)
    • Conjugation kits

      • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
      • APC Conjugation Kit - Lightning-Link® (ab201807)
    • Isotype control

      • Rat IgG - Isotype Control (ab37361)
    • Recombinant Protein

      • Recombinant human CD40 protein (Active) (ab155710)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab22469 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    Flow Cyt
    Mouse
    Application Abreviews Notes
    Flow Cyt
    Use 10µl for 106 cells.

    ab37371 - Rat polyclonal IgG, is suitable for use as an isotype control with this antibody. 

    Notes
    Flow Cyt
    Use 10µl for 106 cells.

    ab37371 - Rat polyclonal IgG, is suitable for use as an isotype control with this antibody. 

    Target

    • Function

      Receptor for TNFSF5/CD40LG.
    • Tissue specificity

      B-cells and in primary carcinomas.
    • Involvement in disease

      Defects in CD40 are the cause of hyper-IgM immunodeficiency syndrome type 3 (HIGM3) [MIM:606843]; also known as hyper-IgM syndrome 3. HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation.
    • Sequence similarities

      Contains 4 TNFR-Cys repeats.
    • Cellular localization

      Secreted and Cell membrane.
    • Target information above from: UniProt accession P25942 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 21939 Mouse
      • SwissProt: P27512 Mouse
      • Unigene: 271833 Mouse
      • Alternative names

        • AI326936 antibody
        • B cell associated molecule CD40 antibody
        • B cell surface antigen CD40 antibody
        • B cell-associated molecule antibody
        • B-cell surface antigen CD40 antibody
        • Bp50 antibody
        • CD 40 antibody
        • CD40 antibody
        • CD40 antigen (TNF receptor superfamily member 5) antibody
        • CD40 antigen antibody
        • CD40 molecule antibody
        • CD40 molecule, TNF receptor superfamily member 5 antibody
        • CD40 protein antibody
        • CD40 type II isoform antibody
        • CD40L receptor antibody
        • CDw40 antibody
        • GP39 antibody
        • HIGM1 antibody
        • IGM antibody
        • IMD3 antibody
        • MGC9013 antibody
        • Nerve growth factor receptor related B lymphocyte activation molecule antibody
        • OTTHUMP00000031699 antibody
        • OTTHUMP00000031700 antibody
        • p50 antibody
        • T-BAM antibody
        • TBAM antibody
        • TNF receptor superfamily member 5 antibody
        • TNFRSF5 antibody
        • TNR5_HUMAN antibody
        • TRAP antibody
        • Tumor necrosis factor receptor superfamily , member 5 antibody
        • Tumor necrosis factor receptor superfamily member 5 antibody
        • Tumor necrosis factor receptor superfamily member 5 precursor antibody
        • Tumor necrosis factor receptor superfamily, member 5, isoform CRA_a antibody
        see all

      Images

      • Flow Cytometry - Anti-CD40 antibody [3/23] (ab22469)
        Flow Cytometry - Anti-CD40 antibody [3/23] (ab22469)
        ab22469 staining CD40 in Mouse Spleen cells by flow cytometric analysis.

      Protocols

      • Flow cytometry protocols
      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (4)

      Publishing research using ab22469? Please let us know so that we can cite the reference in this datasheet.

      ab22469 has been referenced in 4 publications.

      • Benchetrit H  et al. Direct Induction of the Three Pre-implantation Blastocyst Cell Types from Fibroblasts. Cell Stem Cell 24:983-994.e7 (2019). PubMed: 31031139
      • Carriba P & Davies AM CD40 is a major regulator of dendrite growth from developing excitatory and inhibitory neurons. Elife 6:N/A (2017). PubMed: 29111976
      • Donner AJ  et al. CD40 Generation 2.5 Antisense Oligonucleotide Treatment Attenuates Doxorubicin-induced Nephropathy and Kidney Inflammation. Mol Ther Nucleic Acids 4:e265 (2015). PubMed: 26623936
      • Hicks SD & Miller MW Effects of ethanol on transforming growth factor ?1-dependent and -independent mechanisms of neural stem cell apoptosis. Exp Neurol 229:372-80 (2011). WB, ICC ; Mouse . PubMed: 21419121

      Customer reviews and Q&As

      Show All Reviews Q&A
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      Immunohistochemistry (Frozen sections) abreview for Anti-CD40 antibody [3/23]

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Human Tissue sections (Skin)
      Permeabilization
      Yes - 0.5% Triton TBS Ca/Azide
      Specification
      Skin
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
      Fixative
      Paraformaldehyde
      Read More

      Mr. Tyler Crowe

      Verified customer

      Submitted Apr 20 2019

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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