Anti-CD40 antibody [3/23] (ab22469)
![Flow Cytometry - Anti-CD40 antibody [3/23] (ab22469)](https://www.abcam.com/ps/products/22/ab22469/Images/ab22469-31849-anti-cd40-antibody-3-23-flow-cytometry.jpg "Flow Cytometry - Anti-CD40 antibody [3/23] (ab22469)")
Key features and details
- Rat monoclonal [3/23] to CD40
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-CD40 antibody [3/23]
See all CD40 primary antibodies -
Description
Rat monoclonal [3/23] to CD40 -
Host species
Rat -
Specificity
Does not react with normal mouse Ig or with human IgG1 and will stain most mature mouse B cells. Does not cross react with mouse T cells. ab22469 is a powerful activator of normal B cells especially in the presence of IL4. -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Mouse
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Immunogen
Extracellular Domain of Mouse CD40 and the Fc portion of Human IgG1.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
3/23 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab22469 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| Flow Cyt |
Mouse
|
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 10µl for 106 cells.
ab37371 - Rat polyclonal IgG, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
Flow Cyt
Use 10µl for 106 cells. ab37371 - Rat polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Receptor for TNFSF5/CD40LG. -
Tissue specificity
B-cells and in primary carcinomas. -
Involvement in disease
Defects in CD40 are the cause of hyper-IgM immunodeficiency syndrome type 3 (HIGM3) [MIM:606843]; also known as hyper-IgM syndrome 3. HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation. -
Sequence similarities
Contains 4 TNFR-Cys repeats. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 21939 Mouse
- SwissProt: P27512 Mouse
- Unigene: 271833 Mouse
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Alternative names
- AI326936 antibody
- B cell associated molecule CD40 antibody
- B cell surface antigen CD40 antibody
see all
Images
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Flow Cytometry - Anti-CD40 antibody [3/23] (ab22469)ab22469 staining CD40 in Mouse Spleen cells by flow cytometric analysis.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab22469 has been referenced in 4 publications.
- Benchetrit H et al. Direct Induction of the Three Pre-implantation Blastocyst Cell Types from Fibroblasts. Cell Stem Cell 24:983-994.e7 (2019). PubMed: 31031139
- Carriba P & Davies AM CD40 is a major regulator of dendrite growth from developing excitatory and inhibitory neurons. Elife 6:N/A (2017). PubMed: 29111976
- Donner AJ et al. CD40 Generation 2.5 Antisense Oligonucleotide Treatment Attenuates Doxorubicin-induced Nephropathy and Kidney Inflammation. Mol Ther Nucleic Acids 4:e265 (2015). PubMed: 26623936
- Hicks SD & Miller MW Effects of ethanol on transforming growth factor ?1-dependent and -independent mechanisms of neural stem cell apoptosis. Exp Neurol 229:372-80 (2011). WB, ICC ; Mouse . PubMed: 21419121