Anti-CD40 antibody [3/23] (ab22469)
- Datasheet
- References (3)
- Protocols
Overview
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Product nameAnti-CD40 antibody [3/23]
See all CD40 primary antibodies -
DescriptionRat monoclonal [3/23] to CD40
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Host speciesRat
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SpecificityDoes not react with normal mouse Ig or with human IgG1 and will stain most mature mouse B cells. Does not cross react with mouse T cells. ab22469 is a powerful activator of normal B cells especially in the presence of IL4.
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Tested applicationsSuitable for: ELISA, Flow Cyt, IHC-Frmore details
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Species reactivityReacts with: Mouse, Human
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Immunogen
Extracellular Domain of Mouse CD40 and the Fc portion of Human IgG1.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferPreservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4 -
Concentration information loading... -
PurityProtein G purified
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ClonalityMonoclonal
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Clone number3/23
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab22469 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | Use at an assay dependent concentration. | |
| Flow Cyt | Use 10µl for 106 cells. ab37371 - Rat polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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| IHC-Fr | Use at an assay dependent concentration. |
Target
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FunctionReceptor for TNFSF5/CD40LG.
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Tissue specificityB-cells and in primary carcinomas.
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Involvement in diseaseDefects in CD40 are the cause of hyper-IgM immunodeficiency syndrome type 3 (HIGM3) [MIM:606843]; also known as hyper-IgM syndrome 3. HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation.
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Sequence similaritiesContains 4 TNFR-Cys repeats.
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Cellular localizationSecreted and Cell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 958 Human
- Entrez Gene: 21939 Mouse
- Omim: 109535 Human
- SwissProt: P25942 Human
- SwissProt: P27512 Mouse
- Unigene: 472860 Human
- Unigene: 271833 Mouse
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Alternative names
- AI326936 antibody
- B cell associated molecule CD40 antibody
- B cell surface antigen CD40 antibody
see all
Images
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![Flow Cytometry - Anti-CD40 antibody [3/23] (ab22469)](https://www.abcam.com/ps/products/22/ab22469/Images/CD40-Primary-antibodies-ab22469-2.JPG)
Flow Cytometry - Anti-CD40 antibody [3/23] (ab22469)ab22469 staining CD40 in Mouse Spleen cells by flow cytometric analysis.
References
This product has been referenced in:
- Carriba P & Davies AM CD40 is a major regulator of dendrite growth from developing excitatory and inhibitory neurons. Elife 6:N/A (2017). Read more (PubMed: 29111976) »
- Donner AJ et al. CD40 Generation 2.5 Antisense Oligonucleotide Treatment Attenuates Doxorubicin-induced Nephropathy and Kidney Inflammation. Mol Ther Nucleic Acids 4:e265 (2015). Read more (PubMed: 26623936) »
