Anti-CD40 antibody [3/23] (ab22469)
![Flow Cytometry - Anti-CD40 antibody [3/23] (ab22469)](https://www.abcam.com/ps/products/22/ab22469/Images/ab22469-31849-anti-cd40-antibody-3-23-flow-cytometry.jpg "Flow Cytometry - Anti-CD40 antibody [3/23] (ab22469)")
Key features and details
- Rat monoclonal [3/23] to CD40
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Isotype: IgG
Overview
-
Product name
Anti-CD40 antibody [3/23]
See all CD40 primary antibodies -
Description
Rat monoclonal [3/23] to CD40 -
Host species
Rat -
Specificity
Does not react with normal mouse Ig or with human IgG1 and will stain most mature mouse B cells. Does not cross react with mouse T cells. ab22469 is a powerful activator of normal B cells especially in the presence of IL4. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment (Fc-tag) corresponding to Mouse CD40 (extracellular). Extracellular Domain of Mouse CD40 and the Fc portion of Human IgG1.
Database link: P27512 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
3/23 -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab22469 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 10µl for 106 cells.
ab37371 - Rat polyclonal IgG, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
Flow Cyt
Use 10µl for 106 cells. ab37371 - Rat polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
-
Function
Receptor for TNFSF5/CD40LG. -
Tissue specificity
B-cells and in primary carcinomas. -
Involvement in disease
Defects in CD40 are the cause of hyper-IgM immunodeficiency syndrome type 3 (HIGM3) [MIM:606843]; also known as hyper-IgM syndrome 3. HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation. -
Sequence similarities
Contains 4 TNFR-Cys repeats. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 21939 Mouse
- SwissProt: P27512 Mouse
- Unigene: 271833 Mouse
-
Alternative names
- AI326936 antibody
- B cell associated molecule CD40 antibody
- B cell surface antigen CD40 antibody
see all
Images
-
Flow Cytometry - Anti-CD40 antibody [3/23] (ab22469)ab22469 staining CD40 in Mouse Spleen cells by flow cytometric analysis.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (6)
ab22469 has been referenced in 6 publications.
- Lu C et al. Tumor necrosis factor receptor type 1-associated death domain (TRADD) regulates epithelial-mesenchymal transition (EMT), M1/M2 macrophage polarization and ectopic endometrial cysts formation in endometriosis. Ann Transl Med 9:148 (2021). PubMed: 33569450
- Qian J et al. Mechanism of TGF-ß1 inhibiting Kupffer cell immune responses in cholestatic cirrhosis. Exp Ther Med 20:1541-1549 (2020). PubMed: 32742385
- Benchetrit H et al. Direct Induction of the Three Pre-implantation Blastocyst Cell Types from Fibroblasts. Cell Stem Cell 24:983-994.e7 (2019). PubMed: 31031139
- Carriba P & Davies AM CD40 is a major regulator of dendrite growth from developing excitatory and inhibitory neurons. Elife 6:N/A (2017). PubMed: 29111976
- Donner AJ et al. CD40 Generation 2.5 Antisense Oligonucleotide Treatment Attenuates Doxorubicin-induced Nephropathy and Kidney Inflammation. Mol Ther Nucleic Acids 4:e265 (2015). PubMed: 26623936
- Hicks SD & Miller MW Effects of ethanol on transforming growth factor ?1-dependent and -independent mechanisms of neural stem cell apoptosis. Exp Neurol 229:372-80 (2011). WB, ICC ; Mouse . PubMed: 21419121