Anti-CD40 antibody (ab13545)
- Datasheet
- References (5)
- Protocols
Overview
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Product nameAnti-CD40 antibody
See all CD40 primary antibodies -
DescriptionRabbit polyclonal to CD40
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Host speciesRabbit
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SpecificityThe antibody specificity is confirmed by specific peptide inhibition in immunoblotting studies.
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Tested applicationsSuitable for: IHC-P, WBmore details
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Species reactivityReacts with: Mouse, Rat, Sheep, Rabbit, Guinea pig, Hamster, Cow, Human, Pig, Monkey
Predicted to work with: Chicken, Dog
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Immunogen
Synthetic peptide:
CQPVTQEDGKESRISVQERQ
conjugated to KLH, corresponding to amino acids 258-277 of Human CD40. -
Positive control
- HeLa Cell Lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal tonsil.
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General notesStorage below -20°C is not recommended. For maximum product recovery centrifuge the product vial before removing cap. Shipped on gel packs.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
PurityAffinity purified
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Purification notesPeptide affinity purified.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab13545 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 34 kDa). |
Target
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FunctionReceptor for TNFSF5/CD40LG.
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Tissue specificityB-cells and in primary carcinomas.
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Involvement in diseaseDefects in CD40 are the cause of hyper-IgM immunodeficiency syndrome type 3 (HIGM3) [MIM:606843]; also known as hyper-IgM syndrome 3. HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation.
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Sequence similaritiesContains 4 TNFR-Cys repeats.
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Cellular localizationSecreted and Cell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 958 Human
- Entrez Gene: 21939 Mouse
- Entrez Gene: 171369 Rat
- Omim: 109535 Human
- SwissProt: P25942 Human
- SwissProt: P27512 Mouse
- Unigene: 472860 Human
- Unigene: 271833 Mouse
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Alternative names
- AI326936 antibody
- B cell associated molecule CD40 antibody
- B cell surface antigen CD40 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody (ab13545)IHC image of CD40 staining in Human normal tonsil formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab13545, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Western blot - Anti-CD40 antibody (ab13545)Anti-CD40 antibody (ab13545) at 1/1000 dilution +Recombinant human CD40 protein (Fc Chimera Active) (ab83917) at 1 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Exposure time: 8 minutes -
Western blot - Anti-CD40 antibody (ab13545)All lanes : Anti-CD40 antibody (ab13545) at 1/200 dilution
Lane 1 : ESK4 cell lysate
Lane 2 : Molt 4 cell lysate
Predicted band size: 34 kDa -
Western blot - Anti-CD40 antibody (ab13545)All lanes : Anti-CD40 antibody (ab13545) at 1/1000 dilution
Lanes 1-2 : Control human HCAEC cells - whole cell lysate
Lanes 3-4 : Treated human HCAEC cells - whole cell lysate
Secondary
All lanes : HRP conjugated Goat Anti-Rabbit IgG
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Exposure time: 20 seconds
This image is courtesy of an Abreview submitted on 10 August 2005. -
Western blot - Anti-CD40 antibody (ab13545)This image is courtesy of an anonymous AbreviewAll lanes : Anti-CD40 antibody (ab13545) at 1/5000 dilution
Lane 1 : Human B chronic lymphocytic leukaemia cells, nuclear fraction
Lane 2 : Human B chronic lymphocytic leukaemia cells, cytoskeletal fraction
Lane 3 : Human B chronic lymphocytic leukaemia cells, membrane fraction
Lane 4 : Human B chronic lymphocytic leukaemia cells, cytosolic fraction
Lysates/proteins at 18 µg per lane.
Secondary
All lanes : HRP conjugated goat anti-rabbit
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThe primary antibody was incubated with the membrane for 16 hours at 4°C.
References
This product has been referenced in:
- Shoji T et al. Local convection-enhanced delivery of an anti-CD40 agonistic monoclonal antibody induces antitumor effects in mouse glioma models. Neuro Oncol 18:1120-8 (2016). Read more (PubMed: 26917236) »
- Qiu X et al. CD40 ligand induces RIP1-dependent, necroptosis-like cell death in low-grade serous but not serous borderline ovarian tumor cells. Cell Death Dis 6:e1864 (2015). IHC-P ; Human . Read more (PubMed: 26313915) »
