Anti-CD40 antibody (ab13545)
Key features and details
- Rabbit polyclonal to CD40
- Suitable for: WB
- Reacts with: Human, Pig
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-CD40 antibody
See all CD40 primary antibodies -
Description
Rabbit polyclonal to CD40 -
Host species
Rabbit -
Specificity
The antibody specificity is confirmed by specific peptide inhibition in immunoblotting studies. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human, Pig
Predicted to work with: Chicken, Dog
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Immunogen
Synthetic peptide:
CQPVTQEDGKESRISVQERQ
conjugated to KLH, corresponding to amino acids 258-277 of Human CD40. -
General notes
Storage below -20°C is not recommended. For maximum product recovery centrifuge the product vial before removing cap. Shipped on gel packs.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Peptide affinity purified. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab13545 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 34 kDa).
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| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 34 kDa). |
Target
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Function
Receptor for TNFSF5/CD40LG. -
Tissue specificity
B-cells and in primary carcinomas. -
Involvement in disease
Defects in CD40 are the cause of hyper-IgM immunodeficiency syndrome type 3 (HIGM3) [MIM:606843]; also known as hyper-IgM syndrome 3. HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation. -
Sequence similarities
Contains 4 TNFR-Cys repeats. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 958 Human
- Omim: 109535 Human
- SwissProt: P25942 Human
- Unigene: 472860 Human
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Alternative names
- AI326936 antibody
- B cell associated molecule CD40 antibody
- B cell surface antigen CD40 antibody
see all
Images
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Western blot - Anti-CD40 antibody (ab13545)All lanes : Anti-CD40 antibody (ab13545) at 1/200 dilution
Lane 1 : ESK4 cell lysate
Lane 2 : Molt 4 cell lysate
Predicted band size: 34 kDa -
Western blot - Anti-CD40 antibody (ab13545)This image is courtesy of an anonymous AbreviewAll lanes : Anti-CD40 antibody (ab13545) at 1/5000 dilution
Lane 1 : Human B chronic lymphocytic leukaemia cells, nuclear fraction
Lane 2 : Human B chronic lymphocytic leukaemia cells, cytoskeletal fraction
Lane 3 : Human B chronic lymphocytic leukaemia cells, membrane fraction
Lane 4 : Human B chronic lymphocytic leukaemia cells, cytosolic fraction
Lysates/proteins at 18 µg per lane.
Secondary
All lanes : HRP conjugated goat anti-rabbit
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThe primary antibody was incubated with the membrane for 16 hours at 4°C.
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Western blot - Anti-CD40 antibody (ab13545)Anti-CD40 antibody (ab13545) at 1/1000 dilution +Recombinant human CD40 protein (Fc Chimera Active) (ab83917) at 1 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Exposure time: 8 minutes -
Western blot - Anti-CD40 antibody (ab13545)All lanes : Anti-CD40 antibody (ab13545) at 1/1000 dilution
Lanes 1-2 : Control human HCAEC cells - whole cell lysate
Lanes 3-4 : Treated human HCAEC cells - whole cell lysate
Secondary
All lanes : HRP conjugated Goat Anti-Rabbit IgG
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Exposure time: 20 seconds
This image is courtesy of an Abreview submitted on 10 August 2005.
Datasheets and documents
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SDS download
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Datasheet download
References (15)
ab13545 has been referenced in 15 publications.
- Wolf NI et al. Metachromatic leukodystrophy and transplantation: remyelination, no cross-correction. Ann Clin Transl Neurol 7:169-180 (2020). PubMed: 31967741
- Zhan T et al. Cangrelor alleviates bleomycin-induced pulmonary fibrosis by inhibiting platelet activation in mice. Mol Immunol 120:83-92 (2020). PubMed: 32106023
- Zhou YB et al. Injection of CD40 DNA vaccine ameliorates the autoimmune pathology of non-obese diabetic mice with Sjögren's syndrome. Immunol Lett 226:62-70 (2020). PubMed: 32707129
- Castelucci BG et al. Recruitment of monocytes and mature macrophages in mouse pubic symphysis relaxation during pregnancy and postpartum recovery†. Biol Reprod 101:466-477 (2019). PubMed: 31201427
- Liu Z et al. A NIK-SIX signalling axis controls inflammation by targeted silencing of non-canonical NF-?B. Nature 568:249-253 (2019). PubMed: 30894749
Customer reviews and Q&As
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