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    cd40-antibody-ab13545.pdf

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Immunology Adaptive Immunity B Cells CD
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Anti-CD40 antibody (ab13545)

  • Datasheet
  • SDS
Reviews (7) Submit a question References (18)

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Western blot - Anti-CD40 antibody (ab13545)
  • Western blot - Anti-CD40 antibody (ab13545)
  • Western blot - Anti-CD40 antibody (ab13545)
  • Western blot - Anti-CD40 antibody (ab13545)

Key features and details

  • Rabbit polyclonal to CD40
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Sheep, Rabbit, Guinea pig, Hamster, Cow, Human, Pig, Monkey
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-CD40 antibody [EPR20735] (ab224639)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-CD40 antibody
    See all CD40 primary antibodies
  • Description

    Rabbit polyclonal to CD40
  • Host species

    Rabbit
  • Specificity

    The antibody specificity is confirmed by specific peptide inhibition in immunoblotting studies.
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Sheep, Rabbit, Guinea pig, Hamster, Cow, Human, Pig, Monkey
    Predicted to work with: Chicken, Dog
  • Immunogen

    Synthetic peptide:

    CQPVTQEDGKESRISVQERQ

    conjugated to KLH, corresponding to amino acids 258-277 of Human CD40.
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    Storage below -20°C is not recommended. For maximum product recovery centrifuge the product vial before removing cap. Shipped on gel packs.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: PBS, 50% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    Peptide affinity purified.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • B Cells
    • CD
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Innate and adaptive immunity
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • B Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Dendritic Cell Lineage

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human CD40 protein (Active) (ab155710)
  • Related Products

    • Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab13545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (5)
Use at an assay dependent concentration.
WB (2)
1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 34 kDa).
Notes
IHC-P
Use at an assay dependent concentration.
WB
1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 34 kDa).

Target

  • Function

    Receptor for TNFSF5/CD40LG.
  • Tissue specificity

    B-cells and in primary carcinomas.
  • Involvement in disease

    Defects in CD40 are the cause of hyper-IgM immunodeficiency syndrome type 3 (HIGM3) [MIM:606843]; also known as hyper-IgM syndrome 3. HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation.
  • Sequence similarities

    Contains 4 TNFR-Cys repeats.
  • Cellular localization

    Secreted and Cell membrane.
  • Target information above from: UniProt accession P25942 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 958 Human
    • Entrez Gene: 21939 Mouse
    • Entrez Gene: 171369 Rat
    • Omim: 109535 Human
    • SwissProt: P25942 Human
    • SwissProt: P27512 Mouse
    • Unigene: 472860 Human
    • Unigene: 271833 Mouse
    • Alternative names

      • AI326936 antibody
      • B cell associated molecule CD40 antibody
      • B cell surface antigen CD40 antibody
      • B cell-associated molecule antibody
      • B-cell surface antigen CD40 antibody
      • Bp50 antibody
      • CD 40 antibody
      • CD40 antibody
      • CD40 antigen (TNF receptor superfamily member 5) antibody
      • CD40 antigen antibody
      • CD40 molecule antibody
      • CD40 molecule, TNF receptor superfamily member 5 antibody
      • CD40 protein antibody
      • CD40 type II isoform antibody
      • CD40L receptor antibody
      • CDw40 antibody
      • GP39 antibody
      • HIGM1 antibody
      • IGM antibody
      • IMD3 antibody
      • MGC9013 antibody
      • Nerve growth factor receptor related B lymphocyte activation molecule antibody
      • OTTHUMP00000031699 antibody
      • OTTHUMP00000031700 antibody
      • p50 antibody
      • T-BAM antibody
      • TBAM antibody
      • TNF receptor superfamily member 5 antibody
      • TNFRSF5 antibody
      • TNR5_HUMAN antibody
      • TRAP antibody
      • Tumor necrosis factor receptor superfamily , member 5 antibody
      • Tumor necrosis factor receptor superfamily member 5 antibody
      • Tumor necrosis factor receptor superfamily member 5 precursor antibody
      • Tumor necrosis factor receptor superfamily, member 5, isoform CRA_a antibody
      see all

    Images

    • Western blot - Anti-CD40 antibody (ab13545)
      Western blot - Anti-CD40 antibody (ab13545)
      All lanes : Anti-CD40 antibody (ab13545) at 1/200 dilution

      Lane 1 : ESK4 cell lysate
      Lane 2 : Molt 4 cell lysate

      Predicted band size: 34 kDa

    • Western blot - Anti-CD40 antibody (ab13545)
      Western blot - Anti-CD40 antibody (ab13545)This image is courtesy of an anonymous Abreview
      All lanes : Anti-CD40 antibody (ab13545) at 1/5000 dilution

      Lane 1 : Human B chronic lymphocytic leukaemia cells, nuclear fraction
      Lane 2 : Human B chronic lymphocytic leukaemia cells, cytoskeletal fraction
      Lane 3 : Human B chronic lymphocytic leukaemia cells, membrane fraction
      Lane 4 : Human B chronic lymphocytic leukaemia cells, cytosolic fraction

      Lysates/proteins at 18 µg per lane.

      Secondary
      All lanes : HRP conjugated goat anti-rabbit

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 34 kDa
      Observed band size: 42 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      The primary antibody was incubated with the membrane for 16 hours at 4°C.

      See Abreview

    • Western blot - Anti-CD40 antibody (ab13545)
      Western blot - Anti-CD40 antibody (ab13545)
      Anti-CD40 antibody (ab13545) at 1/1000 dilution + Recombinant human CD40 protein (Fc Chimera Active) (ab83917) at 1 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 34 kDa


      Exposure time: 8 minutes
    • Western blot - Anti-CD40 antibody (ab13545)
      Western blot - Anti-CD40 antibody (ab13545)
      All lanes : Anti-CD40 antibody (ab13545) at 1/1000 dilution

      Lanes 1-2 : Control human HCAEC cells - whole cell lysate
      Lanes 3-4 : Treated human HCAEC cells - whole cell lysate

      Secondary
      All lanes : HRP conjugated Goat Anti-Rabbit IgG

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 34 kDa
      Observed band size: 45 kDa why is the actual band size different from the predicted?


      Exposure time: 20 seconds


      This image is courtesy of an Abreview submitted on 10 August 2005.

      See Abreview

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (18)

    Publishing research using ab13545? Please let us know so that we can cite the reference in this datasheet.

    ab13545 has been referenced in 18 publications.

    • Liu C  et al. Genistein-3'-sodium sulfonate Attenuates Neuroinflammation in Stroke Rats by Down-Regulating Microglial M1 Polarization through a7nAChR-NF-?B Signaling Pathway. Int J Biol Sci 17:1088-1100 (2021). PubMed: 33867831
    • Wolf NI  et al. Metachromatic leukodystrophy and transplantation: remyelination, no cross-correction. Ann Clin Transl Neurol 7:169-180 (2020). PubMed: 31967741
    • Zhan T  et al. Cangrelor alleviates bleomycin-induced pulmonary fibrosis by inhibiting platelet activation in mice. Mol Immunol 120:83-92 (2020). PubMed: 32106023
    • Zhou YB  et al. Injection of CD40 DNA vaccine ameliorates the autoimmune pathology of non-obese diabetic mice with Sjögren's syndrome. Immunol Lett 226:62-70 (2020). PubMed: 32707129
    • Zhang S  et al. Renal Fibrosis Is Significantly Attenuated Following Targeted Disruption of Cd40 in Experimental Renal Ischemia. J Am Heart Assoc 9:e014072 (2020). PubMed: 32200719
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-7 of 7 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD40 antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Brain tissue)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate (0.01M) pH=6
    Permeabilization
    Yes - 0.05% Tween
    Specification
    Brain tissue
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More

    Boyd Kenkhuis

    Verified customer

    Submitted Aug 22 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD40 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (spleen)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
    Permeabilization
    No
    Specification
    spleen
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    10% NBF
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 28 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD40 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (skin)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
    Permeabilization
    No
    Specification
    skin
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 27 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD40 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (human tonsil)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Dako Low pH
    Permeabilization
    No
    Specification
    human tonsil
    Blocking step
    Flex endogenouse peroxidase block as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 19 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD40 antibody

    Excellent
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Dako Flex peroxidase as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Low pH
    Sample
    Human Tissue sections (Malignant melanoma/lymphocytes)
    Specification
    Malignant melanoma/lymphocytes
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 20 2015

    Western blot abreview for Anti-CD40 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - other (Human B chronic lymphocytic leukaemia cells)
    Loading amount
    18 µg
    Specification
    Human B chronic lymphocytic leukaemia cells
    Gel Running Conditions
    Reduced Denaturing (12% Tris-Glycine Gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 22 2008

    Western blot abreview for Anti-CD40 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HCAEC)
    Specification
    HCAEC
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 10 2005

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