Anti-CD40 antibody (ab65853)

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Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab65853 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 31 kDa).

Target

  • Function

    Receptor for TNFSF5/CD40LG.

  • Tissue specificity

    B-cells and in primary carcinomas.

  • Involvement in disease

    Defects in CD40 are the cause of hyper-IgM immunodeficiency syndrome type 3 (HIGM3) [MIM:606843]; also known as hyper-IgM syndrome 3. HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation.

  • Sequence similarities

    Contains 4 TNFR-Cys repeats.

  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • AI326936 antibody
      • B cell associated molecule CD40 antibody
      • B cell surface antigen CD40 antibody
      • B cell-associated molecule antibody
      • B-cell surface antigen CD40 antibody
      • Bp50 antibody
      • CD 40 antibody
      • CD40 antibody
      • CD40 antigen (TNF receptor superfamily member 5) antibody
      • CD40 antigen antibody
      • CD40 molecule antibody
      • CD40 molecule, TNF receptor superfamily member 5 antibody
      • CD40 protein antibody
      • CD40 type II isoform antibody
      • CD40L receptor antibody
      • CDw40 antibody
      • GP39 antibody
      • HIGM1 antibody
      • IGM antibody
      • IMD3 antibody
      • MGC9013 antibody
      • Nerve growth factor receptor related B lymphocyte activation molecule antibody
      • OTTHUMP00000031699 antibody
      • OTTHUMP00000031700 antibody
      • p50 antibody
      • T-BAM antibody
      • TBAM antibody
      • TNF receptor superfamily member 5 antibody
      • TNFRSF5 antibody
      • TNR5_HUMAN antibody
      • TRAP antibody
      • Tumor necrosis factor receptor superfamily , member 5 antibody
      • Tumor necrosis factor receptor superfamily member 5 antibody
      • Tumor necrosis factor receptor superfamily member 5 precursor antibody
      • Tumor necrosis factor receptor superfamily, member 5, isoform CRA_a antibody
      see all

    Images

    • Western blot - Anti-CD40 antibody (ab65853)
      Western blot - Anti-CD40 antibody (ab65853)
      All lanes : Anti-CD40 antibody (ab65853)

      Lane 1 : Recombinant Mouse CD 40 Protein at 0.01 µg
      Lane 2 : Recombinant Mouse CD 40 Protein at 0.005 µg
      Lane 3 : Recombinant Mouse CD 40 Protein at 0.0025 µg

      Predicted band size: 31 kDa

    References

    This product has been referenced in:

    • Jakovljevic M  et al. Induction of NTPDase1/CD39 by Reactive Microglia and Macrophages Is Associated With the Functional State During EAE. Front Neurosci 13:410 (2019). Read more (PubMed: 31105520) »
    • Manzo-Merino J  et al. The Human Papillomavirus (HPV) E6 Oncoprotein Regulates CD40 Expression via the AT-Hook Transcription Factor AKNA. Cancers (Basel) 10:N/A (2018). Read more (PubMed: 30562965) »
    See all 3 Publications for this product

    Publishing research using ab65853? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Question

    I just tried ab13545. It works very well. Could you continue send me this one as replacement? Thank you very much!

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    Answer

    Thank you for your email.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with ab13545.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

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    Question

    Human samples
    secondary checked, it's ok
    dilution 1/100
    incubation O/N 4ºC
    5% milk blocking.

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    Answer

    Thank you for contacting us.

    The details provided on the phone will enable us to investigate this case and will provide us with vital information for monitoring product quality.

    As mentioned over the phone, I would suggest to try 5% BSA solutionfor the blocking step as well as for the incubation of the primary and the secondary antibodies.

    Should the suggestions not improve the results, please do let me know.

    In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund.

    I hope this information is helpful, and I thank you for your cooperation.

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