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    cd40-antibody-ab65853.pdf

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Immunology Adaptive Immunity B Cells CD
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Anti-CD40 antibody (ab65853)

  • Datasheet
  • SDS
Submit a review Q&A (2)References (3)

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Abpromise

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Find out more.

Western blot - Anti-CD40 antibody (ab65853)

    Key features and details

    • Rabbit polyclonal to CD40
    • Suitable for: WB
    • Reacts with: Mouse
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-CD40 antibody
      See all CD40 primary antibodies
    • Description

      Rabbit polyclonal to CD40
    • Host species

      Rabbit
    • Tested Applications & Species

      Application Species
      WB
      Mouse
      See all applications and species data
    • Immunogen

      Synthetic peptide corresponding to Mouse CD40 aa 22-36.
      Sequence:

      QCVTCSDKQYLHDGQ


      Database link: P27512
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Lyophilized:0.2ml of distilled water will yield a concentration of 500µg/ml.
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      Constituents: Dibasic monohydrogen sodium phosphate, 50% Glycerol, Sodium chloride
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Immunology
      • Adaptive Immunity
      • B Cells
      • CD
      • Cardiovascular
      • Atherosclerosis
      • Vascular Inflammation
      • Innate and adaptive immunity
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • B Lymphocytic Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Myeloid
      • Dendritic Cell Lineage

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab65853 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Mouse
    Application Abreviews Notes
    WB
    Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 31 kDa).
    Notes
    WB
    Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 31 kDa).

    Target

    • Function

      Receptor for TNFSF5/CD40LG.
    • Tissue specificity

      B-cells and in primary carcinomas.
    • Involvement in disease

      Defects in CD40 are the cause of hyper-IgM immunodeficiency syndrome type 3 (HIGM3) [MIM:606843]; also known as hyper-IgM syndrome 3. HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation.
    • Sequence similarities

      Contains 4 TNFR-Cys repeats.
    • Cellular localization

      Secreted and Cell membrane.
    • Target information above from: UniProt accession P25942 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 21939 Mouse
      • SwissProt: P27512 Mouse
      • Unigene: 271833 Mouse
      • Alternative names

        • AI326936 antibody
        • B cell associated molecule CD40 antibody
        • B cell surface antigen CD40 antibody
        • B cell-associated molecule antibody
        • B-cell surface antigen CD40 antibody
        • Bp50 antibody
        • CD 40 antibody
        • CD40 antibody
        • CD40 antigen (TNF receptor superfamily member 5) antibody
        • CD40 antigen antibody
        • CD40 molecule antibody
        • CD40 molecule, TNF receptor superfamily member 5 antibody
        • CD40 protein antibody
        • CD40 type II isoform antibody
        • CD40L receptor antibody
        • CDw40 antibody
        • GP39 antibody
        • HIGM1 antibody
        • IGM antibody
        • IMD3 antibody
        • MGC9013 antibody
        • Nerve growth factor receptor related B lymphocyte activation molecule antibody
        • OTTHUMP00000031699 antibody
        • OTTHUMP00000031700 antibody
        • p50 antibody
        • T-BAM antibody
        • TBAM antibody
        • TNF receptor superfamily member 5 antibody
        • TNFRSF5 antibody
        • TNR5_HUMAN antibody
        • TRAP antibody
        • Tumor necrosis factor receptor superfamily , member 5 antibody
        • Tumor necrosis factor receptor superfamily member 5 antibody
        • Tumor necrosis factor receptor superfamily member 5 precursor antibody
        • Tumor necrosis factor receptor superfamily, member 5, isoform CRA_a antibody
        see all

      Images

      • Western blot - Anti-CD40 antibody (ab65853)
        Western blot - Anti-CD40 antibody (ab65853)
        All lanes : Anti-CD40 antibody (ab65853)

        Lane 1 : Recombinant Mouse CD 40 Protein at 0.01 µg
        Lane 2 : Recombinant Mouse CD 40 Protein at 0.005 µg
        Lane 3 : Recombinant Mouse CD 40 Protein at 0.0025 µg

        Predicted band size: 31 kDa

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (3)

      Publishing research using ab65853? Please let us know so that we can cite the reference in this datasheet.

      ab65853 has been referenced in 3 publications.

      • Jakovljevic M  et al. Induction of NTPDase1/CD39 by Reactive Microglia and Macrophages Is Associated With the Functional State During EAE. Front Neurosci 13:410 (2019). PubMed: 31105520
      • Manzo-Merino J  et al. The Human Papillomavirus (HPV) E6 Oncoprotein Regulates CD40 Expression via the AT-Hook Transcription Factor AKNA. Cancers (Basel) 10:N/A (2018). PubMed: 30562965
      • Cai H  et al. Neuroprotective effects of bajijiasu against cognitive impairment induced by amyloid-ß in APP/PS1 mice. Oncotarget 8:92621-92634 (2017). PubMed: 29190943

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-2 of 2 Abreviews or Q&A

      Question

      I just tried ab13545. It works very well. Could you continue send me this one as replacement? Thank you very much!

      Read More

      Abcam community

      Verified customer

      Asked on Aug 29 2012

      Answer

      Thank you for your email.

      I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with ab13545.

      To check the status of the order please contact our Customer Service team and reference this number.

      Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

      I wish you the best of luck with your research.

      Read More

      Abcam Scientific Support

      Answered on Aug 29 2012

      Question

      Human samples
      secondary checked, it's ok
      dilution 1/100
      incubation O/N 4ºC
      5% milk blocking.

      Read More

      Abcam community

      Verified customer

      Asked on Aug 10 2012

      Answer

      Thank you for contacting us.

      The details provided on the phone will enable us to investigate this case and will provide us with vital information for monitoring product quality.

      As mentioned over the phone, I would suggest to try 5% BSA solutionfor the blocking step as well as for the incubation of the primary and the secondary antibodies.

      Should the suggestions not improve the results, please do let me know.

      In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund.

      I hope this information is helpful, and I thank you for your cooperation.

      Read More

      Abcam Scientific Support

      Answered on Aug 10 2012

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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