Anti-CD40 antibody (ab65853)
Key features and details
- Rabbit polyclonal to CD40
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-CD40 antibody
See all CD40 primary antibodies -
Description
Rabbit polyclonal to CD40 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide corresponding to Mouse CD40 aa 22-36.
Sequence:QCVTCSDKQYLHDGQ
Database link: P27512 -
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Lyophilized:0.2ml of distilled water will yield a concentration of 500µg/ml. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituents: Dibasic monohydrogen sodium phosphate, 50% Glycerol, Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab65853 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Mouse
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 31 kDa).
|
| Notes |
|---|
|
WB
Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 31 kDa). |
Target
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Function
Receptor for TNFSF5/CD40LG. -
Tissue specificity
B-cells and in primary carcinomas. -
Involvement in disease
Defects in CD40 are the cause of hyper-IgM immunodeficiency syndrome type 3 (HIGM3) [MIM:606843]; also known as hyper-IgM syndrome 3. HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation. -
Sequence similarities
Contains 4 TNFR-Cys repeats. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 21939 Mouse
- SwissProt: P27512 Mouse
- Unigene: 271833 Mouse
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Alternative names
- AI326936 antibody
- B cell associated molecule CD40 antibody
- B cell surface antigen CD40 antibody
see all
Images
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Western blot - Anti-CD40 antibody (ab65853)All lanes : Anti-CD40 antibody (ab65853)
Lane 1 : Recombinant Mouse CD 40 Protein at 0.01 µg
Lane 2 : Recombinant Mouse CD 40 Protein at 0.005 µg
Lane 3 : Recombinant Mouse CD 40 Protein at 0.0025 µg
Predicted band size: 31 kDa
References (3)
ab65853 has been referenced in 3 publications.
- Jakovljevic M et al. Induction of NTPDase1/CD39 by Reactive Microglia and Macrophages Is Associated With the Functional State During EAE. Front Neurosci 13:410 (2019). PubMed: 31105520
- Manzo-Merino J et al. The Human Papillomavirus (HPV) E6 Oncoprotein Regulates CD40 Expression via the AT-Hook Transcription Factor AKNA. Cancers (Basel) 10:N/A (2018). PubMed: 30562965
- Cai H et al. Neuroprotective effects of bajijiasu against cognitive impairment induced by amyloid-ß in APP/PS1 mice. Oncotarget 8:92621-92634 (2017). PubMed: 29190943
