Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityIon Exchange Chromatography
Purification notesSterile-filtered through 0.22 µm and treated to remove endotoxins. Carrier and preservative free
Our Abpromise guarantee covers the use of ab47021 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 5 - 10 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 30 kDa).|
|Flow Cyt||Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionReceptor for TNFSF5/CD40LG.
Tissue specificityB-cells and in primary carcinomas.
Involvement in diseaseDefects in CD40 are the cause of hyper-IgM immunodeficiency syndrome type 3 (HIGM3) [MIM:606843]; also known as hyper-IgM syndrome 3. HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation.
Sequence similaritiesContains 4 TNFR-Cys repeats.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- AI326936 antibody
- B cell associated molecule CD40 antibody
- B cell surface antigen CD40 antibody
A typical staining pattern with the B-B20 monoclonal antibody of lymphocytes FACS analysis of CD40 expression in Human lymphocytes using ab47021.
All lanes : Anti-CD40 antibody [B-B20] (ab47021) at 5 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 30 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 17 kDa, 30 kDa. We are unsure as to the identity of these extra bands.
CD40 is a transmembrane glycoprotein that runs at 48 to 50 kDa on SDS page gels.
This product has been referenced in:
- Pan W et al. Resveratrol Protects against TNF-a-Induced Injury in Human Umbilical Endothelial Cells through Promoting Sirtuin-1-Induced Repression of NF-KB and p38 MAPK. PLoS One 11:e0147034 (2016). Human . Read more (PubMed: 26799794) »
- Holl V et al. Stimulation of HIV-1 replication in immature dendritic cells in contact with primary CD4 T or B lymphocytes. J Virol 84:4172-82 (2010). Flow Cyt ; Human . Read more (PubMed: 20147388) »