The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 - 10 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 30 kDa).
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Receptor for TNFSF5/CD40LG.
B-cells and in primary carcinomas.
Involvement in disease
Defects in CD40 are the cause of hyper-IgM immunodeficiency syndrome type 3 (HIGM3) [MIM:606843]; also known as hyper-IgM syndrome 3. HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation.
Pan W et al. Resveratrol Protects against TNF-a-Induced Injury in Human Umbilical Endothelial Cells through Promoting Sirtuin-1-Induced Repression of NF-KB and p38 MAPK. PLoS One11:e0147034 (2016).
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Holl V et al. Stimulation of HIV-1 replication in immature dendritic cells in contact with primary CD4 T or B lymphocytes. J Virol84:4172-82 (2010).
Read more (PubMed: 20147388) »