Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18005-4] to CD40
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Mouse
Product nameAnti-CD40 antibody [EPR18005-4]
See all CD40 primary antibodies
DescriptionRabbit monoclonal [EPR18005-4] to CD40
Specificityab212058 recognises a different epitope to ab188181.
Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
Species reactivityReacts with: Mouse
Recombinant fragment within Mouse CD40 aa 1-200. The exact sequence is proprietary.
Database link: P27512
- WB: A20 whole cell lysate. ICC/IF: A20 cells. Flow Cyt: A20 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab212058 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 32 kDa).|
FunctionReceptor for TNFSF5/CD40LG.
Tissue specificityB-cells and in primary carcinomas.
Involvement in diseaseDefects in CD40 are the cause of hyper-IgM immunodeficiency syndrome type 3 (HIGM3) [MIM:606843]; also known as hyper-IgM syndrome 3. HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation.
Sequence similaritiesContains 4 TNFR-Cys repeats.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- AI326936 antibody
- B cell associated molecule CD40 antibody
- B cell surface antigen CD40 antibody
Flow cytometric analysis of A20 (mouse reticulum sarcoma cell line) cell line labeling CD40 with ab212058 at 1/600 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
Immunofluorescent analysis of 100% methanol-fixed A20 (mouse reticulum sarcoma cell line) cells labeling CD40 with ab212058 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Membranous staining on A20 cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
Anti-CD40 antibody [EPR18005-4] (ab212058) at 1/1000 dilution + A20 (mouse reticulum sarcoma cell line) whole cell lysate at 10 µg
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 32 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
Blocking/Dilution buffer: 5% NFDM/TBST.
The molecular mass observed is consistent with the literature (PMID: 23404288 and PMID: 16709811).
ab212058 has not yet been referenced specifically in any publications.