Recombinant
RabMAb

Recombinant Anti-CD40 antibody [EPR20540] (ab213205)

Overview

  • Product name

    Anti-CD40 antibody [EPR20540]
    See all CD40 primary antibodies
  • Description

    Rabbit monoclonal [EPR20540] to CD40
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human CD40 aa 1-200. The exact sequence is proprietary.
    Database link: P25942

  • Positive control

    • WB: Human tonsil, lymph node and lymphoma lysates; Raji and Daudi whole cell lysates. IHC-P: Human tonsil and large B cell lymphoma tissues.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab213205 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/2000. Detects a band of approximately 42 kDa (predicted molecular weight: 30 kDa).

Target

  • Function

    Receptor for TNFSF5/CD40LG.
  • Tissue specificity

    B-cells and in primary carcinomas.
  • Involvement in disease

    Defects in CD40 are the cause of hyper-IgM immunodeficiency syndrome type 3 (HIGM3) [MIM:606843]; also known as hyper-IgM syndrome 3. HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation.
  • Sequence similarities

    Contains 4 TNFR-Cys repeats.
  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • AI326936 antibody
    • B cell associated molecule CD40 antibody
    • B cell surface antigen CD40 antibody
    • B cell-associated molecule antibody
    • B-cell surface antigen CD40 antibody
    • Bp50 antibody
    • CD 40 antibody
    • CD40 antibody
    • CD40 antigen (TNF receptor superfamily member 5) antibody
    • CD40 antigen antibody
    • CD40 molecule antibody
    • CD40 molecule, TNF receptor superfamily member 5 antibody
    • CD40 protein antibody
    • CD40 type II isoform antibody
    • CD40L receptor antibody
    • CDw40 antibody
    • GP39 antibody
    • HIGM1 antibody
    • IGM antibody
    • IMD3 antibody
    • MGC9013 antibody
    • Nerve growth factor receptor related B lymphocyte activation molecule antibody
    • OTTHUMP00000031699 antibody
    • OTTHUMP00000031700 antibody
    • p50 antibody
    • T-BAM antibody
    • TBAM antibody
    • TNF receptor superfamily member 5 antibody
    • TNFRSF5 antibody
    • TNR5_HUMAN antibody
    • TRAP antibody
    • Tumor necrosis factor receptor superfamily , member 5 antibody
    • Tumor necrosis factor receptor superfamily member 5 antibody
    • Tumor necrosis factor receptor superfamily member 5 precursor antibody
    • Tumor necrosis factor receptor superfamily, member 5, isoform CRA_a antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD40 with ab213205 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. 

    Membranous and cytoplasmic staining on germinal center of human tonsil is observed [PMID: 10360965] [PMID: 7507299] [PMID:24452203].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • All lanes : Anti-CD40 antibody [EPR20540] (ab213205) at 1/2000 dilution

    Lane 1 : Human tonsil lysate
    Lane 2 : Human lymph node lysate
    Lane 3 : Human lymphoma lysate
    Lane 4 : Raji (Human Burkitt's lymphoma cell line) whole cell lysate
    Lane 5 : Daudi (Human Burkitt's lymphoma cell line) whole cell lysate
    Lane 6 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lanes 1-3 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution
    Lanes 4-6 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 30 kDa
    Observed band size: 42 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1,2,3 and 4: 30 seconds; Lane 5,6 and 7: 5 seconds.

    The molecular weight observed is consistent with the literature (PMID: 23404288);

    Negative control: Jurkat (PMID:10498643; PMID:15708600).

  • Immunohistochemical analysis of paraffin-embedded human large B cell lymphoma tissue labeling CD40 with ab213205 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Membranous staining on human large B cell lymphoma is observed [PMID: 7507299].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

ab213205 has not yet been referenced specifically in any publications.

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