The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 1µg for 105-8 cells.
In a final volume of 100 μl.
Use at an assay dependent concentration.
Receptor for TNFSF5/CD40LG.
B-cells and in primary carcinomas.
Involvement in disease
Defects in CD40 are the cause of hyper-IgM immunodeficiency syndrome type 3 (HIGM3) [MIM:606843]; also known as hyper-IgM syndrome 3. HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation.
Flow cytometric analysis of BALB/c splenocytes with 0.5 µg Armenian Hamster IgG isotype control (open histogram) or 0.5 µg ab171209 (filled histogram) followed by Anti-Armenian Hamster IgM FITC. Total viable cells were used for analysis.