Overview

  • Product name
  • Description
    Rabbit polyclonal to CD41
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: a wide range of other species
  • Immunogen

    Synthetic peptide corresponding to Human CD41 (N terminal). Synthetic peptide derived from the N terminal domain of Human CD41.
    Database link: P08514

  • General notes

    This product was changed from whole antiserum to Protein A purified on 15th June 2016. The following lots are from whole antiserum and are still in stock as of 15th June 2016, GR264406-3, GR250036-5 , GR250036-4. Lot numbers higher than GR264406-3, will be Protein A purified. Please note that the dilutions may need to be adjusted accordingly.

Properties

Applications

Our Abpromise guarantee covers the use of ab63983 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/5000. Predicted molecular weight: 113 kDa.
ICC/IF Use at an assay dependent concentration. PubMed: 20942599
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. It recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial cell surface.
  • Tissue specificity
    Isoform 1 and isoform 2 were identified in platelets and megakaryocytes, but not in reticulocytes or in Jurkat and U937 white blood cell line. Isoform 3 is expressed by leukemia, prostate adenocarcinoma and melanoma cells but not by platelets or normal prostate or breast epithelial cells.
  • Involvement in disease
    Defects in ITGA2B are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. GT is the most common inherited disease of platelets. It is an autosomal recessive disorder characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I, platelets show absence of the glycoprotein IIb/beta-3 complexes at their surface and lack fibrinogen and clot retraction capability. In type II, the platelets express the glycoprotein IIb/beta-3 complex at reduced levels (5-20% controls), have detectable amounts of fibrinogen, and have low or moderate clot retraction capability. The platelets of GT 'variants' have normal or near normal (60-100%) expression of dysfunctional receptors.
  • Sequence similarities
    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • antigen CD41 antibody
    • BDPLT16 antibody
    • BDPLT2 antibody
    • CD41 antibody
    • CD41B antibody
    • form 2 antibody
    • GP2B antibody
    • GPalpha IIb antibody
    • GPIIb antibody
    • GT antibody
    • GTA antibody
    • HPA3 antibody
    • Integrin alpha 2b antibody
    • Integrin alpha IIb antibody
    • Integrin alpha-IIb light chain antibody
    • Integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41) antibody
    • ITA2B_HUMAN antibody
    • Itga2b antibody
    • ITGAB antibody
    • platelet fibrinogen receptor, alpha subunit antibody
    • platelet glycoprotein IIb of IIb/IIIa complex antibody
    • Platelet membrane glycoprotein IIb antibody
    • platelet specific antigen BAK antibody
    • PPP1R93 antibody
    see all

Images

  • All lanes : Anti-CD41 antibody (ab63983) at 1/10000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : HepG2 whole cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal at 1/20000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 113 kDa
    Observed band size: 113 kDa


    Exposure time: 15 seconds

    See Abreview

  • All lanes : Anti-CD41 antibody (ab63983) at 1/5000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : HepG2 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/20000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 113 kDa
    Observed band size: 113 kDa


    Exposure time: 10 seconds

    See Abreview

References

This product has been referenced in:
  • Lee J  et al. Fully Dedifferentiated Chondrocytes Expanded in Specific Mesenchymal Stem Cell Growth Medium with FGF2 Obtains Mesenchymal Stem Cell Phenotype In Vitro but Retains Chondrocyte Phenotype In Vivo. Cell Transplant 26:1673-1687 (2017). Read more (PubMed: 29251111) »
  • Myllymäki MN  et al. Notch Downregulation and Extramedullary Erythrocytosis in Hypoxia-Inducible Factor Prolyl 4-Hydroxylase 2-Deficient Mice. Mol Cell Biol 37:N/A (2017). Read more (PubMed: 27821476) »

See all 15 Publications for this product

Customer reviews and Q&As

Thank you for confirming those details. I have now arranged for a new vial of ab63983 to be sent to you free of charge. This is on the order number 1162584.

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Thank you for contacting us yesterday and reporting the problems encountered with the Anti-CD41/Integrin alpha 2b antibody (ab63983).

As discussed over the phone, I am able to offer you an alternative lot of ab63983 if you would like to try i...

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I have tried to find some alternative suggestions for your experiment that you may want to consider. I found no further antibodies against VE-Cadherin that might be suitable and a...

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I am writing to inquire if the heat induced antigen retrieval step has helped to improve the results? I would be pleased to receive an update on how these experiments are progressing.
If the antibody is still not working as stated on the datasheet...

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Following your answer on the antigen retrieval, I would like to confirm one more point. Indeed, have the slides been de-paraffinized by sequential incubations? If the slides have not been deparaffinized, this c...

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I'm sorry to hear that this lot of ab63983 is giving you and your colleagues trouble. We have not had a recent complaint regarding this lot number. However, since it does not appear to be working as expected, I wo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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