For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    cd41-antibody-epr4330-ab134131.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Cardiovascular Angiogenesis Adhesion / ECM Integrins
Share by email
RecombinantRabMAb

Recombinant Anti-CD41 antibody [EPR4330] (ab134131)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (16)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD41 antibody [EPR4330] (ab134131)
  • Western blot - Anti-CD41 antibody [EPR4330] (ab134131)
  • Immunocytochemistry/ Immunofluorescence - Anti-CD41 antibody [EPR4330] (ab134131)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD41 antibody [EPR4330] (ab134131)
  • Western blot - Anti-CD41 antibody [EPR4330] (ab134131)
  • Anti-CD41 antibody [EPR4330] (ab134131)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4330] to CD41
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free HRP

You may also be interested in

Kit
Product image
F-actin Staining Kit - Green Fluorescence - Cytopainter (ab112125)
Primary
Product image
Anti-Integrin beta 3 antibody [ERP17507] (ab179473)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-CD41 antibody [EPR4330]
    See all CD41 primary antibodies
  • Description

    Rabbit monoclonal [EPR4330] to CD41
  • Host species

    Rabbit
  • Specificity

    Mouse reactivity is only guaranteed for IHC-P. 

  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human CD41 aa 50-150 (extracellular). The exact sequence is proprietary. Synthetic peptide corresponding to residues in the extracellular domain in Human CD41.
    Database link: P08514

  • Positive control

    • Human platelet, fetal liver and placenta lysates; Human spleen tissue
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% Tris glycine, 0.88% Sodium chloride, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR4330
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Adhesion / ECM
    • Integrins
    • Cardiovascular
    • Blood
    • Platelets
    • Immunology
    • Cell Type Markers
    • CD
    • Platelets
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Integrins
    • Alpha
    • Stem Cells
    • Hematopoietic Progenitors
    • Hematopoietic Stem Cells
    • Murine Lineage Negative
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Thrombocytic Lineage

Associated products

  • Alternative Versions

    • HRP Anti-CD41 antibody [EPR4330] (ab194981)
    • Anti-CD41 antibody [EPR4330] - Low endotoxin, Azide free (ab229448)
    • Anti-CD41 antibody [EPR4330] - BSA and Azide free (ab271906)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab134131 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use at an assay dependent concentration.
WB
1/1000 - 1/10000. Predicted molecular weight: 113 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
ICC/IF
Use at an assay dependent concentration.
WB
1/1000 - 1/10000. Predicted molecular weight: 113 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Application notes
Is unsuitable for IP.

Target

  • Function

    Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. It recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial cell surface.
  • Tissue specificity

    Isoform 1 and isoform 2 were identified in platelets and megakaryocytes, but not in reticulocytes or in Jurkat and U937 white blood cell line. Isoform 3 is expressed by leukemia, prostate adenocarcinoma and melanoma cells but not by platelets or normal prostate or breast epithelial cells.
  • Involvement in disease

    Defects in ITGA2B are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. GT is the most common inherited disease of platelets. It is an autosomal recessive disorder characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I, platelets show absence of the glycoprotein IIb/beta-3 complexes at their surface and lack fibrinogen and clot retraction capability. In type II, the platelets express the glycoprotein IIb/beta-3 complex at reduced levels (5-20% controls), have detectable amounts of fibrinogen, and have low or moderate clot retraction capability. The platelets of GT 'variants' have normal or near normal (60-100%) expression of dysfunctional receptors.
  • Sequence similarities

    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P08514 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3674 Human
    • Entrez Gene: 16399 Mouse
    • Omim: 607759 Human
    • SwissProt: P08514 Human
    • SwissProt: Q9QUM0 Mouse
    • Unigene: 411312 Human
    • Alternative names

      • antigen CD41 antibody
      • BDPLT16 antibody
      • BDPLT2 antibody
      • CD41 antibody
      • CD41B antibody
      • form 2 antibody
      • GP2B antibody
      • GPalpha IIb antibody
      • GPIIb antibody
      • GT antibody
      • GTA antibody
      • HPA3 antibody
      • Integrin alpha 2b antibody
      • Integrin alpha IIb antibody
      • Integrin alpha-IIb light chain antibody
      • Integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41) antibody
      • ITA2B_HUMAN antibody
      • Itga2b antibody
      • ITGAB antibody
      • platelet fibrinogen receptor, alpha subunit antibody
      • platelet glycoprotein IIb of IIb/IIIa complex antibody
      • Platelet membrane glycoprotein IIb antibody
      • platelet specific antigen BAK antibody
      • PPP1R93 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD41 antibody [EPR4330] (ab134131)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD41 antibody [EPR4330] (ab134131)

      Ab134131 staining CD41 in paraffin embedded Human bone marrow tissue sections by Immunohistochemistry. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody at 1/2000 dilution (0.30 μg/ml).  A ready to use Goat Anti-rabbit IgG H&L (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Cytoplasmic staining in megakaryocytes of human bone marrow (PMID: 27128503; PMID: 23667055).

    • Western blot - Anti-CD41 antibody [EPR4330] (ab134131)
      Western blot - Anti-CD41 antibody [EPR4330] (ab134131)
      All lanes : Anti-CD41 antibody [EPR4330] (ab134131) at 0.6 µg/ml

      Lane 1 : HEL (human Erythroleukemia erythroblast) whole cell lysate
      Lane 2 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

      Predicted band size: 113 kDa


      Exposure time: 3 minutes


      Blocking and diluting buffer: 5% NFDM/TBST. 

      Negative control: K-562 (PMID 2458779)

    • Immunocytochemistry/ Immunofluorescence - Anti-CD41 antibody [EPR4330] (ab134131)
      Immunocytochemistry/ Immunofluorescence - Anti-CD41 antibody [EPR4330] (ab134131)

      Ab134131 staining CD41 in HEL (human Erythroleukemia erythroblast) cells by ICC/IF (Immunocytochemistry/Immunofluorescence. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% TritonX-100. Samples were incubated with primary antibody at 1/100 dilution (6 μg/ml). An AlexaFluor® 488 Goat anti-Rabbit (ab150077) was used as the secondary antibody at 1/1000 dilution (2 μg/ml). Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) , ab195889 was used a counterstain antibody at 1/200 dilution (2.5 μg/ml). DAPI was used as a nuclear counterstain. Confocal image showing membranous staining in HEL cell line.

      Negative control: K562( PMID 2458779)

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD41 antibody [EPR4330] (ab134131)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD41 antibody [EPR4330] (ab134131)

      Ab134131 staining CD41 paraffin embedded Mouse bone marrow tissue sections by Immunohistochemistry. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody at 1/500 dilution (1.21 μg/ml). A ready to use Goat Anti-rabbit IgG H&L (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Cytoplasmic staining in megakaryocytes of mouse bone marrow (PMID: 27128503).

    • Western blot - Anti-CD41 antibody [EPR4330] (ab134131)
      Western blot - Anti-CD41 antibody [EPR4330] (ab134131)
      All lanes : Anti-CD41 antibody [EPR4330] (ab134131) at 1/1000 dilution

      Lane 1 : Human platelet lysate
      Lane 2 : Human fetal liver lysate
      Lane 3 : Human placenta lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti rabbit at 1/2000 dilution

      Predicted band size: 113 kDa

    • Anti-CD41 antibody [EPR4330] (ab134131)
      Anti-CD41 antibody [EPR4330] (ab134131)

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (16)

    Publishing research using ab134131? Please let us know so that we can cite the reference in this datasheet.

    ab134131 has been referenced in 16 publications.

    • Li C  et al. KLF4 down-regulation resulting from TLR4 promotion of ERK1/2 phosphorylation underpins inflammatory response in sepsis. J Cell Mol Med 25:2013-2024 (2021). PubMed: 33369167
    • Xiao F  et al. Platelets and Regulatory T Cells May Induce a Type 2 Immunity That Is Conducive to the Progression and Fibrogenesis of Endometriosis. Front Immunol 11:610963 (2020). PubMed: 33381124
    • Maier A  et al. Molecular magnetic resonance imaging of activated platelets allows noninvasive detection of early myocarditis in mice. Sci Rep 10:13211 (2020). PubMed: 32764735
    • Cheng X  et al. Prothrombotic effects of high uric acid in mice via activation of MEF2C-dependent NF-?B pathway by upregulating let-7c. Aging (Albany NY) 12:17976-17989 (2020). PubMed: 32960786
    • Zhu W  et al. CD41-deficient exosomes from non-traumatic femoral head necrosis tissues impair osteogenic differentiation and migration of mesenchymal stem cells. Cell Death Dis 11:293 (2020). PubMed: 32341357
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review

    Filter by Application

    Filter by Species

    Filter by Ratings

    Immunohistochemistry (Frozen sections) abreview for Anti-CD41 antibody [EPR4330]

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (kidney-tissue)
    Permeabilization
    No
    Specification
    kidney-tissue
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Gideon Schäfer

    Verified customer

    Submitted Apr 27 2021

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2023 Abcam plc. All rights reserved.