Recombinant Anti-CD41 antibody [EPR4330] (ab134131)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD41 antibody [EPR4330] (ab134131)](https://www.abcam.com/ps/products/134/ab134131/Images/ab134131-315109-anti-cd41-antibody-epr4330-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD41 antibody [EPR4330] (ab134131)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4330] to CD41
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-CD41 antibody [EPR4330]
See all CD41 primary antibodies -
Description
Rabbit monoclonal [EPR4330] to CD41 -
Host species
Rabbit -
Specificity
Mouse reactivity is only guaranteed for IHC-P.
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Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human CD41 aa 50-150 (extracellular). The exact sequence is proprietary. Synthetic peptide corresponding to residues in the extracellular domain in Human CD41.
Database link: P08514 -
Positive control
- Human platelet, fetal liver and placenta lysates; Human spleen tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% Tris glycine, 0.88% Sodium chloride, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4330 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134131 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
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| WB |
1/1000 - 1/10000. Predicted molecular weight: 113 kDa.
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| Notes |
|---|
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ICC/IF
Use at an assay dependent concentration. |
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WB
1/1000 - 1/10000. Predicted molecular weight: 113 kDa. |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. It recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial cell surface. -
Tissue specificity
Isoform 1 and isoform 2 were identified in platelets and megakaryocytes, but not in reticulocytes or in Jurkat and U937 white blood cell line. Isoform 3 is expressed by leukemia, prostate adenocarcinoma and melanoma cells but not by platelets or normal prostate or breast epithelial cells. -
Involvement in disease
Defects in ITGA2B are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. GT is the most common inherited disease of platelets. It is an autosomal recessive disorder characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I, platelets show absence of the glycoprotein IIb/beta-3 complexes at their surface and lack fibrinogen and clot retraction capability. In type II, the platelets express the glycoprotein IIb/beta-3 complex at reduced levels (5-20% controls), have detectable amounts of fibrinogen, and have low or moderate clot retraction capability. The platelets of GT 'variants' have normal or near normal (60-100%) expression of dysfunctional receptors. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3674 Human
- Entrez Gene: 16399 Mouse
- Omim: 607759 Human
- SwissProt: P08514 Human
- SwissProt: Q9QUM0 Mouse
- Unigene: 411312 Human
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Alternative names
- antigen CD41 antibody
- BDPLT16 antibody
- BDPLT2 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD41 antibody [EPR4330] (ab134131)
Ab134131 staining CD41 in paraffin embedded Human bone marrow tissue sections by Immunohistochemistry. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody at 1/2000 dilution (0.30 μg/ml). A ready to use Goat Anti-rabbit IgG H&L (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Cytoplasmic staining in megakaryocytes of human bone marrow (PMID: 27128503; PMID: 23667055).
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![Western blot - Anti-CD41 antibody [EPR4330] (ab134131)](https://www.abcam.com/ps/products/134/ab134131/Images/ab134131-315101-anti-cd41-antibody-epr4330-western-blot.jpg)
Western blot - Anti-CD41 antibody [EPR4330] (ab134131)All lanes : Anti-CD41 antibody [EPR4330] (ab134131) at 0.6 µg/ml
Lane 1 : HEL (human Erythroleukemia erythroblast) whole cell lysate
Lane 2 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 113 kDa
Exposure time: 3 minutesBlocking and diluting buffer: 5% NFDM/TBST.
Negative control: K-562 (PMID 2458779) -
![Immunocytochemistry/ Immunofluorescence - Anti-CD41 antibody [EPR4330] (ab134131)](https://www.abcam.com/ps/products/134/ab134131/Images/ab134131-315112-anti-cd41-antibody-epr4330-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD41 antibody [EPR4330] (ab134131)Ab134131 staining CD41 in HEL (human Erythroleukemia erythroblast) cells by ICC/IF (Immunocytochemistry/Immunofluorescence. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% TritonX-100. Samples were incubated with primary antibody at 1/100 dilution (6 μg/ml). An AlexaFluor® 488 Goat anti-Rabbit (ab150077) was used as the secondary antibody at 1/1000 dilution (2 μg/ml). Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) , ab195889 was used a counterstain antibody at 1/200 dilution (2.5 μg/ml). DAPI was used as a nuclear counterstain. Confocal image showing membranous staining in HEL cell line.
Negative control: K562( PMID 2458779)
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD41 antibody [EPR4330] (ab134131)](https://www.abcam.com/ps/products/134/ab134131/Images/ab134131-315110-anti-cd41-antibody-epr4330-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD41 antibody [EPR4330] (ab134131)Ab134131 staining CD41 paraffin embedded Mouse bone marrow tissue sections by Immunohistochemistry. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody at 1/500 dilution (1.21 μg/ml). A ready to use Goat Anti-rabbit IgG H&L (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Cytoplasmic staining in megakaryocytes of mouse bone marrow (PMID: 27128503).
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![Western blot - Anti-CD41 antibody [EPR4330] (ab134131)](https://www.abcam.com/ps/products/134/ab134131/Images/ab134131-148364-anti-cd41-antibody-epr4330-western-blot.jpg)
Western blot - Anti-CD41 antibody [EPR4330] (ab134131)All lanes : Anti-CD41 antibody [EPR4330] (ab134131) at 1/1000 dilution
Lane 1 : Human platelet lysate
Lane 2 : Human fetal liver lysate
Lane 3 : Human placenta lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti rabbit at 1/2000 dilution
Predicted band size: 113 kDa -
![Anti-CD41 antibody [EPR4330] (ab134131)](https://www.abcam.com/ps/products/134/ab134131/Images/ab134131-10-benefits-of-recombinant-antibodies.png)
Anti-CD41 antibody [EPR4330] (ab134131)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (16)
ab134131 has been referenced in 16 publications.
- Li C et al. KLF4 down-regulation resulting from TLR4 promotion of ERK1/2 phosphorylation underpins inflammatory response in sepsis. J Cell Mol Med 25:2013-2024 (2021). PubMed: 33369167
- Xiao F et al. Platelets and Regulatory T Cells May Induce a Type 2 Immunity That Is Conducive to the Progression and Fibrogenesis of Endometriosis. Front Immunol 11:610963 (2020). PubMed: 33381124
- Maier A et al. Molecular magnetic resonance imaging of activated platelets allows noninvasive detection of early myocarditis in mice. Sci Rep 10:13211 (2020). PubMed: 32764735
- Cheng X et al. Prothrombotic effects of high uric acid in mice via activation of MEF2C-dependent NF-?B pathway by upregulating let-7c. Aging (Albany NY) 12:17976-17989 (2020). PubMed: 32960786
- Zhu W et al. CD41-deficient exosomes from non-traumatic femoral head necrosis tissues impair osteogenic differentiation and migration of mesenchymal stem cells. Cell Death Dis 11:293 (2020). PubMed: 32341357