Key features and details
- Mouse monoclonal [HIP8] to CD41
- Suitable for: Flow Cyt, IHC-Fr
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-CD41 antibody [HIP8]
See all CD41 primary antibodies
DescriptionMouse monoclonal [HIP8] to CD41
SpecificityAb15021 reconizes the GPIIb-alpha (heavy chain) of CD41. The CD41/CD61 complex is a receptor for fibronectin, fibrinogen, von Willebrand factor, vitronectin and thrombospondin and mediates platelets aggregation. Ab15021 [HIP8] blocks platelet aggregation and completely inhibits ADP, epinephrine, and collagen-induced platelet activation, and partially inhibits ristocetin and thrombin-induced platelet activation.
Tested applicationsSuitable for: Flow Cyt, IHC-Frmore details
Species reactivityReacts with: Human
Full length native protein (purified) corresponding to Human CD41. Full length native protein (purified) (Human).
Database link: P08514
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab15021 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
Can be used at 0.5 mg or less per 100 ml blood (per 106 cells). Tested on human platelets.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IHC-Fr||Use at an assay dependent concentration.|
FunctionIntegrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. It recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial cell surface.
Tissue specificityIsoform 1 and isoform 2 were identified in platelets and megakaryocytes, but not in reticulocytes or in Jurkat and U937 white blood cell line. Isoform 3 is expressed by leukemia, prostate adenocarcinoma and melanoma cells but not by platelets or normal prostate or breast epithelial cells.
Involvement in diseaseDefects in ITGA2B are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. GT is the most common inherited disease of platelets. It is an autosomal recessive disorder characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I, platelets show absence of the glycoprotein IIb/beta-3 complexes at their surface and lack fibrinogen and clot retraction capability. In type II, the platelets express the glycoprotein IIb/beta-3 complex at reduced levels (5-20% controls), have detectable amounts of fibrinogen, and have low or moderate clot retraction capability. The platelets of GT 'variants' have normal or near normal (60-100%) expression of dysfunctional receptors.
Sequence similaritiesBelongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
- Information by UniProt
- antigen CD41 antibody
- BDPLT16 antibody
- BDPLT2 antibody
ab15021 has been referenced in 1 publication.
- Gaertner F et al. Migrating Platelets Are Mechano-scavengers that Collect and Bundle Bacteria. Cell 171:1368-1382.e23 (2017). PubMed: 29195076