Anti-CD41 antibody [M148] (ab11024)
- Datasheet
- References (6)
- Protocols
Overview
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Product nameAnti-CD41 antibody [M148]
See all CD41 primary antibodies -
DescriptionMouse monoclonal [M148] to CD41
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Host speciesMouse
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Specificityab11024 binds to some medulloblastoma and neuroblastoma cells and rhabdomyosarcoma and certain other solid tumours. No binding observed to marrow constituents other than platelets and megakaryocytes.
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Tested applicationsSuitable for: Flow Cyt, ICC/IF, IP, IHC-P, IHC-Frmore details
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Species reactivityReacts with: Human
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Immunogen
Tissue, cells or virus corresponding to Human CD41. Homogenized human medulloblastoma tissue.
Database link: P08514
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferConstituent: PBS
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Concentration information loading...
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PurityProtein A purified
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ClonalityMonoclonal
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Clone numberM148
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MyelomaP3x63-Ag8.653
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IsotypeIgG1
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab11024 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt | Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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ICC/IF | Use at an assay dependent concentration. | |
IP | Use at an assay dependent concentration. | |
IHC-P | Use at an assay dependent concentration. PubMed: 25065646 | |
IHC-Fr | 1/1000. |
Target
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FunctionIntegrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. It recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial cell surface.
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Tissue specificityIsoform 1 and isoform 2 were identified in platelets and megakaryocytes, but not in reticulocytes or in Jurkat and U937 white blood cell line. Isoform 3 is expressed by leukemia, prostate adenocarcinoma and melanoma cells but not by platelets or normal prostate or breast epithelial cells.
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Involvement in diseaseDefects in ITGA2B are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. GT is the most common inherited disease of platelets. It is an autosomal recessive disorder characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I, platelets show absence of the glycoprotein IIb/beta-3 complexes at their surface and lack fibrinogen and clot retraction capability. In type II, the platelets express the glycoprotein IIb/beta-3 complex at reduced levels (5-20% controls), have detectable amounts of fibrinogen, and have low or moderate clot retraction capability. The platelets of GT 'variants' have normal or near normal (60-100%) expression of dysfunctional receptors.
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Sequence similaritiesBelongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats. -
Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 3674 Human
- Omim: 607759 Human
- SwissProt: P08514 Human
- Unigene: 411312 Human
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Alternative names
- antigen CD41 antibody
- BDPLT16 antibody
- BDPLT2 antibody
see all
Images
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Immunohistochemistry (Frozen sections) - Anti-CD41 antibody [M148] (ab11024)This image is courtesy of an abreview submitted by Dr. Ruma Raha-Chowdhury (University of Cambridge, United Kingdom)
IHC-Fr image of C41 staining on human blood film using ab11024 (1:1000). The sample was fixed using paraformaldehyde and permeabilized using 0.1% TritonX in 0.1% PBS. The slides were then blocked with 10% Donkey Serum for 1 hr at 24°C. ab11024 was diluted 1:100 using 0.1% TritonX with 0.1x PBS- 10% Donkeys and slides were incubated with the primary antibody for 4 hours at 24°C. The secondary antibody used was Donkey Polyclonal to rabbit IgG conjugated to Alexa Fuor 568 (1:1000)
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Immunocytochemistry/ Immunofluorescence - Anti-CD41 antibody [M148] (ab11024)This image was submitted by an anonymous abreviewer.
ICC/IF image of ab11024 staining of human platelet cells. The sections were incubated in 10% serum to block non-specific protein-protein interactions and in 0.2% triton X to permeabilise the cells. The sections were then incubated with ab11024 (1:400) for one hour at 20°C, followed by alexa 488 conjugated secondary antibody (green). Platelets were counterstained with rhodamine phalloidin to outline the cellular cytoskeleton (red).
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Overlay histogram showing HL60 cells stained with ab11024 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11024, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed HL60 cells used under the same conditions.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Protocols
References
This product has been referenced in:
- Rambøl MH et al. Recellularization of Decellularized Venous Grafts Using Peripheral Blood: A Critical Evaluation. EBioMedicine 32:215-222 (2018). IHC-Fr ; Human . Read more (PubMed: 29779699) »
- Dhenge A et al. Arachidonic acid and Docosahexanoic acid enhance platelet formation from human apheresis-derived CD34+ cells. Cell Cycle 16:979-990 (2017). Read more (PubMed: 28388313) »