Anti-CD41 antibody [M148] (ab11024)

Reviews (2)Q&A (1)References (9)
Flow Cytometry - Anti-CD41 antibody [M148] (ab11024)
  • Flow Cytometry - Anti-CD41 antibody [M148] (ab11024)

Key features and details

  • Mouse monoclonal [M148] to CD41
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Isotype: IgG2a

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 FITC

Overview

  • Product name

    Anti-CD41 antibody [M148]
    See all CD41 primary antibodies

  • Description

    Mouse monoclonal [M148] to CD41

  • Host species

    Mouse

  • Tested applications

    Suitable for: Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cyt: HEL92.1.7 cells.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab11024 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use a concentration of 0.1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Notes
Flow Cyt
Use a concentration of 0.1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. It recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial cell surface.

  • Tissue specificity

    Isoform 1 and isoform 2 were identified in platelets and megakaryocytes, but not in reticulocytes or in Jurkat and U937 white blood cell line. Isoform 3 is expressed by leukemia, prostate adenocarcinoma and melanoma cells but not by platelets or normal prostate or breast epithelial cells.

  • Involvement in disease

    Defects in ITGA2B are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. GT is the most common inherited disease of platelets. It is an autosomal recessive disorder characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I, platelets show absence of the glycoprotein IIb/beta-3 complexes at their surface and lack fibrinogen and clot retraction capability. In type II, the platelets express the glycoprotein IIb/beta-3 complex at reduced levels (5-20% controls), have detectable amounts of fibrinogen, and have low or moderate clot retraction capability. The platelets of GT 'variants' have normal or near normal (60-100%) expression of dysfunctional receptors.

  • Sequence similarities

    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • antigen CD41 antibody
      • BDPLT16 antibody
      • BDPLT2 antibody
      • CD41 antibody
      • CD41B antibody
      • form 2 antibody
      • GP2B antibody
      • GPalpha IIb antibody
      • GPIIb antibody
      • GT antibody
      • GTA antibody
      • HPA3 antibody
      • Integrin alpha 2b antibody
      • Integrin alpha IIb antibody
      • Integrin alpha-IIb light chain antibody
      • Integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41) antibody
      • ITA2B_HUMAN antibody
      • Itga2b antibody
      • ITGAB antibody
      • platelet fibrinogen receptor, alpha subunit antibody
      • platelet glycoprotein IIb of IIb/IIIa complex antibody
      • Platelet membrane glycoprotein IIb antibody
      • platelet specific antigen BAK antibody
      • PPP1R93 antibody
      see all

    Images

    • Flow Cytometry - Anti-CD41 antibody [M148] (ab11024)
      Flow Cytometry - Anti-CD41 antibody [M148] (ab11024)

      Overlay histograms showing left HEL92.1.7 cells and right Jurkat cells stained with ab11024 (red line). The cells were incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab11024) (1x106 in 100µl at 0.1µg/ml) for 30 min on ice.

      The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150177)was used at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgG1κ (ab170190) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

      Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable single cells.

    • Flow Cytometry - Anti-CD41 antibody [M148] (ab11024)
      Flow Cytometry - Anti-CD41 antibody [M148] (ab11024)

      Overlay histogram showing HL60 cells stained with ab11024 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11024, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed HL60 cells used under the same conditions.

      Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

    References (9)

    Publishing research using ab11024? Please let us know so that we can cite the reference in this datasheet.

    ab11024 has been referenced in 9 publications.

    • Rossi A  et al. Calcium Signaling and Mitochondrial Function in Presenilin 2 Knock-Out Mice: Looking for Any Loss-of-Function Phenotype Related to Alzheimer's Disease. Cells 10:N/A (2021). PubMed: 33494218
    • Rambøl MH  et al. Recellularization of Decellularized Venous Grafts Using Peripheral Blood: A Critical Evaluation. EBioMedicine 32:215-222 (2018). IHC-Fr ; Human . PubMed: 29779699
    • Dhenge A  et al. Arachidonic acid and Docosahexanoic acid enhance platelet formation from human apheresis-derived CD34+ cells. Cell Cycle 16:979-990 (2017). PubMed: 28388313
    • Lohrke J  et al. 18F-GP1, a Novel PET Tracer Designed for High-Sensitivity, Low-Background Detection of Thrombi. J Nucl Med 58:1094-1099 (2017). PubMed: 28302764
    • Zhao H  et al. Inhibition of Megakaryocyte Differentiation by Antibody-Drug Conjugates (ADCs) is Mediated by Macropinocytosis: Implications for ADC-induced Thrombocytopenia. Mol Cancer Ther 16:1877-1886 (2017). PubMed: 28655784
    • Schunke KJ  et al. A novel atherothrombotic model of ischemic stroke induced by injection of collagen into the cerebral vasculature. J Neurosci Methods 239:65-74 (2015). PubMed: 25314906
    • Finnegan S  et al. Synovial membrane protein expression differs between juvenile idiopathic arthritis subtypes in early disease. Arthritis Res Ther 16:R8 (2014). IHC-Fr ; Human . PubMed: 24410838
    • Alonso-Orgaz S  et al. Proteomic characterization of human coronary thrombus in patients with ST-segment elevation acute myocardial infarction. J Proteomics 109C:368-381 (2014). IHC-P ; Human . PubMed: 25065646
    • Jones D  et al. A monoclonal antibody binding to human medulloblastoma cells and to the platelet glycoprotein IIB-IIIA complex. Br J Haematol 57:621-31 (1984). PubMed: 6234927

    Customer reviews and Q&As

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    1-3 of 3 Abreviews or Q&A

    Immunohistochemistry (Frozen sections)

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Sample
    Human Tissue sections (Human blood film)
    Specification
    Human blood film
    Permeabilization
    Yes - 0.1% TritonX in 0.1% PBS
    Fixative
    Paraformaldehyde
    Read More

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted Jun 05 2013

    Immunocytochemistry/ Immunofluorescence abreview for Anti-CD41/Integrin alpha 2b antibody [M148]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (platelet)
    Specification
    platelet
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 0.2% Triton X100, 5 min
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 14 2011

    Question

    Our customer requested discount codes for two antibodies(ab19079 and ab11024).

    Read More
    Answer

    DISCOUNT CODE for ab11024 : ****** Expiration date: DD MM YYYY     I am very pleased to hear you would like to accept our offer and test ab11024 in IHC-P and IHC-Fr in Rat. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for IHC-P and IHC-Fr in Rat. and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.       Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.     Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.     The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.    

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