Overview

  • Product name

    Anti-CD41 antibody [M148] (Alexa Fluor® 647)
    See all CD41 primary antibodies
  • Description

    Mouse monoclonal [M148] to CD41 (Alexa Fluor® 647)
  • Host species

    Mouse
  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Specificity

    This antibody binds to some medulloblastoma and neuroblastoma cells and rhabdomyosarcoma and certain other solid tumours. No binding observed to marrow constituents other than platelets and megakaryocytes.
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Tissue, cells or virus corresponding to Human CD41. Homogenized human medulloblastoma tissue.
    Database link: P08514

  • Positive control

    • Flow cyt: HEL 92.1.7 cells
  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

Applications

Our Abpromise guarantee covers the use of ab233054 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/12500.

Target

  • Function

    Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. It recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial cell surface.
  • Tissue specificity

    Isoform 1 and isoform 2 were identified in platelets and megakaryocytes, but not in reticulocytes or in Jurkat and U937 white blood cell line. Isoform 3 is expressed by leukemia, prostate adenocarcinoma and melanoma cells but not by platelets or normal prostate or breast epithelial cells.
  • Involvement in disease

    Defects in ITGA2B are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. GT is the most common inherited disease of platelets. It is an autosomal recessive disorder characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I, platelets show absence of the glycoprotein IIb/beta-3 complexes at their surface and lack fibrinogen and clot retraction capability. In type II, the platelets express the glycoprotein IIb/beta-3 complex at reduced levels (5-20% controls), have detectable amounts of fibrinogen, and have low or moderate clot retraction capability. The platelets of GT 'variants' have normal or near normal (60-100%) expression of dysfunctional receptors.
  • Sequence similarities

    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • antigen CD41 antibody
    • BDPLT16 antibody
    • BDPLT2 antibody
    • CD41 antibody
    • CD41B antibody
    • form 2 antibody
    • GP2B antibody
    • GPalpha IIb antibody
    • GPIIb antibody
    • GT antibody
    • GTA antibody
    • HPA3 antibody
    • Integrin alpha 2b antibody
    • Integrin alpha IIb antibody
    • Integrin alpha-IIb light chain antibody
    • Integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41) antibody
    • ITA2B_HUMAN antibody
    • Itga2b antibody
    • ITGAB antibody
    • platelet fibrinogen receptor, alpha subunit antibody
    • platelet glycoprotein IIb of IIb/IIIa complex antibody
    • Platelet membrane glycoprotein IIb antibody
    • platelet specific antigen BAK antibody
    • PPP1R93 antibody
    see all

Images

  • Overlay histogram showing left HEL92.1.7 positive cells and right negative Jurkat cells stained with ab233054 (red line). The cells were incubated in 1x PBS containing 10 % normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab233054)  (1x106 in 100 μl at 0.04 μg/ml (1/12500)) for 30 min on ice. Isotype control antibody (black line) was mouse IgG2a kappa; Alexa Fluor® 647  used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.

References

ab233054 has not yet been referenced specifically in any publications.

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