Key features and details
- Rat monoclonal [MWReg30] to CD41 (PE)
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Product nameAnti-CD41 antibody [MWReg30] (PE)
See all CD41 primary antibodies
DescriptionRat monoclonal [MWReg30] to CD41 (PE)
ConjugationPE. Ex: 488nm, Em: 575nm
Specificityab33660 recognizes integrin alpha IIb subunit CD41.
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Mouse
The details of the immunogen for this antibody are not available.
Clone MWReg30 has been reported to inhibit PMA induced aggregation in vitro and to induce hypothermia in vivo. The azide free format is recommended for functional studies.
Purified IgG conjugated to R. Phycoerythrin (RPE).
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.40
Preservative: 0.09% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityProtein G purified
Purification notesPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Primary antibody notesClone MWReg30 has been reported to inhibit PMA induced aggregation in vitro and to induce hypothermia in vivo. The azide free format is recommended for functional studies.
Our Abpromise guarantee covers the use of ab33660 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 10µl for 106 cells.
Use 10µl of the suggested working dilution to label 106 cells in 100µl.
Note: The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors.
ab154449 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionIntegrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. It recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial cell surface.
Tissue specificityIsoform 1 and isoform 2 were identified in platelets and megakaryocytes, but not in reticulocytes or in Jurkat and U937 white blood cell line. Isoform 3 is expressed by leukemia, prostate adenocarcinoma and melanoma cells but not by platelets or normal prostate or breast epithelial cells.
Involvement in diseaseDefects in ITGA2B are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. GT is the most common inherited disease of platelets. It is an autosomal recessive disorder characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I, platelets show absence of the glycoprotein IIb/beta-3 complexes at their surface and lack fibrinogen and clot retraction capability. In type II, the platelets express the glycoprotein IIb/beta-3 complex at reduced levels (5-20% controls), have detectable amounts of fibrinogen, and have low or moderate clot retraction capability. The platelets of GT 'variants' have normal or near normal (60-100%) expression of dysfunctional receptors.
Sequence similaritiesBelongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
- Information by UniProt
- antigen CD41 antibody
- BDPLT16 antibody
- BDPLT2 antibody
ab33660 has been referenced in 2 publications.
- Mobarrez F et al. Microparticles in the blood of patients with systemic lupus erythematosus (SLE): phenotypic characterization and clinical associations. Sci Rep 6:36025 (2016). PubMed: 27777414
- Nieswandt B et al. Acute systemic reaction and lung alterations induced by an antiplatelet integrin gpIIb/IIIa antibody in mice. Blood 94:684-93 (1999). PubMed: 10397735