Overview

  • Product name

    Anti-CD42a/GP-IX antibody [EP5294]
    See all CD42a/GP-IX primary antibodies
  • Description

    Rabbit monoclonal [EP5294] to CD42a/GP-IX
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CD42a/GP-IX aa 50-150. The exact sequence is proprietary.

  • Positive control

    • Human platelet and Peripheral Blood Mononuclear Cell (PBMC) lysates.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Previously labelled as CD42a. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 3.03 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EP5294
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab133573 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).
  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function

      The GPIb-V-IX complex functions as the vWF receptor and mediates vWF-dependent platelet adhesion to blood vessels. The adhesion of platelets to injured vascular surfaces in the arterial circulation is a critical initiating event in hemostasis. GP-IX may provide for membrane insertion and orientation of GP-Ib.
    • Involvement in disease

      Defects in GP9 are a cause of Bernard-Soulier syndrome (BSS) [MIM:231200]; also known as giant platelet disease (GPD). BSS patients have unusually large platelets and have a clinical bleeding tendency.
    • Sequence similarities

      Contains 1 LRR (leucine-rich) repeat.
      Contains 1 LRRCT domain.
      Contains 1 LRRNT domain.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • CD42a antibody
      • Glycoprotein 9 antibody
      • Glycoprotein IX antibody
      • Glycoprotein IX (platelet) antibody
      • Glycoprotein IX precursor antibody
      • GP IX antibody
      • GP-IX antibody
      • GP9 antibody
      • GPIX antibody
      • GPIX_HUMAN antibody
      • Platelet glycoprotein IX antibody
      see all

    Images

    • All lanes : Anti-CD42a/GP-IX antibody [EP5294] (ab133573) at 1/10000 dilution

      Lane 1 : Human platelet lysate
      Lane 2 : PBMC lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 19 kDa
      Observed band size: 19 kDa

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab133573 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab133573.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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