Anti-CD42b antibody [6H6H10] (ab233794)
Key features and details
- Mouse monoclonal [6H6H10] to CD42b
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD42b antibody [6H6H10]
See all CD42b primary antibodies -
Description
Mouse monoclonal [6H6H10] to CD42b -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human CD42b aa 1-200 (extracellular). (Expressed in E.coli).
Database link: P07359 -
Positive control
- WB: HL-60, MOLT-4 and Ramos celll lysates; CD42b (aa 17-183)-hIgGFc transfected HEK-293 cell lysate; Human CD42b (aa 17-183) recombinant protein. Flow Cyt: HL-60 and Jurkat cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
6H6H10 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233794 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2000. Predicted molecular weight: 72 kDa.
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Flow Cyt |
1/200 - 1/400.
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Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 72 kDa. |
Flow Cyt
1/200 - 1/400. |
Target
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Function
GP-Ib, a surface membrane protein of platelets, participates in the formation of platelet plugs by binding to the A1 domain of vWF, which is already bound to the subendothelium. -
Involvement in disease
Non-arteritic anterior ischemic optic neuropathy
Bernard-Soulier syndrome
Bernard-Soulier syndrome A2, autosomal dominant
Pseudo-von Willebrand disease -
Sequence similarities
Contains 7 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain. -
Post-translational
modificationsGlycocalicin, which is approximately coextensive with the extracellular part of the molecule, is cleaved off by calpain during platelet lysis. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 2811 Human
- Omim: 606672 Human
- SwissProt: P07359 Human
- Unigene: 1472 Human
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Alternative names
- Antigen CD42b alpha antibody
- Antigen CD42b-alpha antibody
- BDPLT1 antibody
see all
Images
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Anti-CD42b antibody [6H6H10] (ab233794) at 1/500 dilution + Human CD42b (aa 17-183) recombinant protein
Predicted band size: 72 kDaExpected MW is 44.2 kDa.
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All lanes : Anti-CD42b antibody [6H6H10] (ab233794) at 1/500 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : CD42b (aa 17-183)-hIgGFc transfected HEK-293 cell lysate
Predicted band size: 72 kDa -
All lanes : Anti-CD42b antibody [6H6H10] (ab233794) at 1/500 dilution
Lane 1 : HL-60 (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : MOLT-4 (human lymphoblastic leukemia cell line) cell lysate
Lane 3 : Ramos (human Burkitt's lymphoma cell line) cell lysate
Predicted band size: 72 kDa -
Flow cytometric analysis of HL-60 (human promyelocytic leukemia cell line) cells labeling CD42b with ab233794 at 1/200 dilution (green) compared with a negative control (red).
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Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cells labeling CD42b with ab233794 at 1/200 dilution (green) compared with a negative control (red).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab233794 has not yet been referenced specifically in any publications.