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    cd42b-antibody-ak2-ab61402.pdf

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Cardiovascular Blood Platelets
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Recombinant

Recombinant Anti-CD42b antibody [AK2] (ab61402)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (1)

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Immunocytochemistry/ Immunofluorescence - Anti-CD42b antibody [AK2] (ab61402)
  • Immunohistochemistry (Frozen sections) - Anti-CD42b antibody [AK2] (ab61402)
  • Flow Cytometry - Anti-CD42b antibody [AK2] (ab61402)
  • Immunocytochemistry/ Immunofluorescence - Anti-CD42b antibody [AK2] (ab61402)
  • Flow Cytometry - Anti-CD42b antibody [AK2] (ab61402)
  • Immunohistochemistry (Frozen sections) - Anti-CD42b antibody [AK2] (ab61402)
  • Anti-CD42b antibody [AK2] (ab61402)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [AK2] to CD42b
  • Suitable for: Flow Cyt, IHC-Fr, ICC
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Protein
Product image
Recombinant Human CD42b protein (ab158557)
Secondary
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Goat Anti-Mouse IgG H&L (HRP) (ab205719)
Primary
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Anti-Nac1 antibody (ab29047)

View more associated products

Overview

  • Product name

    Anti-CD42b antibody [AK2]
    See all CD42b primary antibodies
  • Description

    Mouse monoclonal [AK2] to CD42b
  • Host species

    Mouse
  • Tested applications

    Suitable for: Flow Cyt, IHC-Fr, ICCmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Tissue, cells or virus corresponding to Human CD42b. Human platelets.

  • Positive control

    • IHC-Fr: Human Spleen frozen tissue sections. Flow Cyt: Human whole blood and PBMCs. ICC: HEL and Mouse splenocyte cells.
  • General notes

    This product has switched from a hybridoma to recombinant production method on 08th March 2021.

    Clone AK2 has been reported to block the binding of von Willebrand Factor (VWF) to platelets.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Primary antibody notes

    Clone AK2 has been reported to block the binding of von Willebrand Factor (VWF) to platelets.
  • Clonality

    Monoclonal
  • Clone number

    AK2
  • Isotype

    IgG1
  • Research areas

    • Cardiovascular
    • Blood
    • Platelets
    • Immunology
    • Cell Type Markers
    • CD
    • Platelets

Associated products

  • Alternative Versions

    • PE Anti-CD42b antibody [AK2] (ab232912)
    • Anti-CD42b antibody [AK2] - BSA and Azide free (ab252264)
    • Biotin Anti-CD42b antibody [AK2] (ab30400)
    • FITC Anti-CD42b antibody [AK2] (ab30401)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant Human CD42b protein (ab158557)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab61402 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use a concentration of 10 µg/ml.
IHC-Fr
Use a concentration of 1 µg/ml.
ICC
1/100.
Notes
Flow Cyt
Use a concentration of 10 µg/ml.
IHC-Fr
Use a concentration of 1 µg/ml.
ICC
1/100.

Target

  • Function

    GP-Ib, a surface membrane protein of platelets, participates in the formation of platelet plugs by binding to the A1 domain of vWF, which is already bound to the subendothelium.
  • Involvement in disease

    Non-arteritic anterior ischemic optic neuropathy
    Bernard-Soulier syndrome
    Bernard-Soulier syndrome A2, autosomal dominant
    Pseudo-von Willebrand disease
  • Sequence similarities

    Contains 7 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.
  • Post-translational
    modifications

    Glycocalicin, which is approximately coextensive with the extracellular part of the molecule, is cleaved off by calpain during platelet lysis.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P07359 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2811 Human
    • Entrez Gene: 14723 Mouse
    • Omim: 606672 Human
    • SwissProt: P07359 Human
    • SwissProt: O35930 Mouse
    • Unigene: 1472 Human
    • Alternative names

      • Antigen CD42b alpha antibody
      • Antigen CD42b-alpha antibody
      • BDPLT1 antibody
      • BDPLT3 antibody
      • BSS antibody
      • CD 42b antibody
      • CD42b alpha antibody
      • CD42b antibody
      • CD42b antigen antibody
      • DBPLT3 antibody
      • Glycocalicin antibody
      • Glycoprotein Ib (platelet) alpha polypeptide antibody
      • Glycoprotein Ibalpha antibody
      • GP Ib alpha antibody
      • GP-Ib alpha antibody
      • GP1B antibody
      • Gp1ba antibody
      • GP1BA_HUMAN antibody
      • GPIb alpha antibody
      • GPIb-alpha antibody
      • GPIbA antibody
      • MGC34595 antibody
      • Platelet glycoprotein Ib alpha chain antibody
      • Platelet glycoprotein Ib alpha polypeptide antibody
      • Platelet membrane glycoprotein 1b alpha subunit antibody
      • VWDP antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-CD42b antibody [AK2] (ab61402)
      Immunocytochemistry/ Immunofluorescence - Anti-CD42b antibody [AK2] (ab61402)

      Immunocytochemistry analysis of HEL (human Erythroleukemia erythroblast) labelling CD42b with ab61402 at 1/100 (6.3 µg/mL). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat anti Mouse IgG (Alexa Fluor® 488, ab150113) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. Cells were counterstained with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker 1/1000 (1 µg/mL), followed by Goat anti-Rabbit, AlexaFluor®594 ab150080 at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
      Confocal image showing membranous staining in HEL cells.

    • Immunohistochemistry (Frozen sections) - Anti-CD42b antibody [AK2] (ab61402)
      Immunohistochemistry (Frozen sections) - Anti-CD42b antibody [AK2] (ab61402)

      Immunohistochemistry image of CD42b staining in a section of frozen normal human spleen performed on a Leica BOND™ system using the standard protocol.

      The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab61402, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjµgated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.


      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions,primary antibody concentration and antibody incubation times.

    • Flow Cytometry - Anti-CD42b antibody [AK2] (ab61402)
      Flow Cytometry - Anti-CD42b antibody [AK2] (ab61402)

      Flow cytometry staining of Human peripheral blood mononuclear cell (PBMC) with ab61402 (right) or mouse IgG isotype control (left) at 1/500 dilution, followed by Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution. Cells were stained with mouse IgG (Left) or ab61402 (Right). Then stained with anti-CD41 conjugated to APC.

      Gated on viable cells.

    • Immunocytochemistry/ Immunofluorescence - Anti-CD42b antibody [AK2] (ab61402)
      Immunocytochemistry/ Immunofluorescence - Anti-CD42b antibody [AK2] (ab61402)

      Immunocytochemistry analysis of Mouse splenocytes labelling CD42b with ab61402 at 1/100 (6.3 µg/mL). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat anti Mouse IgG (Alexa Fluor® 488, ab150113) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. Cells were counterstained with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker 1/1000 (1 µg/mL), followed by Goat anti-Rabbit, AlexaFluor®594 ab150080 at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
      Confocal image showing membranous staining in mouse splenocytes.

    • Flow Cytometry - Anti-CD42b antibody [AK2] (ab61402)
      Flow Cytometry - Anti-CD42b antibody [AK2] (ab61402)

      Flow cytometry staining of human whole blood with ab61402 (right) or mouse IgG1 kappa; (ab170190) isotype (left). Red blood cells of 200 µl blood were lysed, then cells were incubated for 30 min on ice in 1x PBS containing 10 µg/ml human IgG and 10 µll normal goat serum to block Fc receptors and non-specific protein-protein interaction followed by the antibody (ab61402) or mouse IgG1 kappa; (ab170190) isotype (1x106 in 100 µl; at 1 µg/ml) for 30 min on ice.

      The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150117) was used at 1/2000 dilution for 30 min on ice.

      The cells were simultaneously stained with CD41/CD61  APC. Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on granulocytes.

    • Immunohistochemistry (Frozen sections) - Anti-CD42b antibody [AK2] (ab61402)
      Immunohistochemistry (Frozen sections) - Anti-CD42b antibody [AK2] (ab61402)

      Negative control image. Immunohistochemistry image of CD42b staining in a section of frozen normal human cerebral cortex performed on a Leica BOND™ system using the standard protocol.

      The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab61402, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjµgated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    • Anti-CD42b antibody [AK2] (ab61402)
      Anti-CD42b antibody [AK2] (ab61402)

    Protocols

    • Flow cytometry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab61402? Please let us know so that we can cite the reference in this datasheet.

    ab61402 has been referenced in 1 publication.

    • Ting LH  et al. Contractile forces in platelet aggregates under microfluidic shear gradients reflect platelet inhibition and bleeding risk. Nat Commun 10:1204 (2019). PubMed: 30867419

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    Customer has kindly contacted us for further information regarding the use of this product in blocking application.

    Read More

    Abcam community

    Verified customer

    Asked on Sep 26 2012

    Answer

    Thank you for contacting us regarding ourAnti-CD42b antibody [AK2],ab61402. While we have not done blocking experiments ourselves with this product, the clone [AK2] was first created in the 1980s and has a number of published references in which it was found toblock the binding of von Willebrand Factor (VWF) to platelets as including in: WardCM,AndrewsRK, SmithAI, BerndtMC. Mocarhagin, a novel cobra venom metalloproteinase, cleaves the platelet von Willebrand factor receptor glycoprotein Ibalpha: identification of the sulfated tyrosine/anionic sequence Tyr-276-Glu-282 of glycoprotein Ibalpha as a binding site for von Willebrand factor and alpha-thrombin. Biochemistry.1996;35:4929-4938. Further papers may be found on Google Scholar:http://scholar.google.com/scholar?hl=en&q=AK2+von+willebrand&btnG=&as_sdt=1%2C38&as_sdtp= After review of some of those papers including the following I have seen thatconcentration of the antibody used in these experiments appears to be between 5-10ug/ml. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. Use our products? Submit an Abreview. Earn rewards! https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Sep 26 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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