Anti-CD42b antibody [AK2] (ab61402)

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Key features and details

  • Mouse monoclonal [AK2] to CD42b
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-CD42b antibody [AK2]
    See all CD42b primary antibodies

  • Description

    Mouse monoclonal [AK2] to CD42b

  • Host species

    Mouse

  • Tested applications

    Suitable for: Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control

    • Human peripheral blood platelets.

  • General notes


    Clone AK2 has been reported to block the binding of von Willebrand Factor (VWF) to platelets.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Primary antibody notes

    Clone AK2 has been reported to block the binding of von Willebrand Factor (VWF) to platelets.

  • Clonality

    Monoclonal

  • Clone number

    AK2

  • Isotype

    IgG1

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab61402 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/490.

Use 10µl of the suggested working dilution to label 100µl whole blood.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Relevance

    CD42b is a platelet activation marker involved in the process of coagulation as an aggregating factor. It interacts with extracellular matrix as well as with adhesion molecules. Its expression and activation seems to be regulated by VEGF and PDGF.

  • Cellular localization

    Cell Membrane

  • Database links

    • Alternative names

      • Antigen CD42b alpha antibody
      • BDPLT1 antibody
      • BDPLT3 antibody
      • BSS antibody
      • CD 42b antibody
      • CD42b alpha antibody
      • CD42B antibody
      • CD42b antigen antibody
      • DBPLT3 antibody
      • GLYCOCALICIN antibody
      • Glycoprotein Ib (platelet) alpha polypeptide antibody
      • Glycoprotein Ibalpha antibody
      • GP Ib alpha antibody
      • GP1B antibody
      • GP1BA antibody
      • GPIb alpha antibody
      • GPIbA antibody
      • MGC34595 antibody
      • Platelet glycoprotein Ib alpha chain antibody
      • Platelet glycoprotein Ib alpha polypeptide antibody
      • Platelet membrane glycoprotein 1b alpha subunit antibody
      • VWDP antibody
      see all

    References (1)

    Publishing research using ab61402? Please let us know so that we can cite the reference in this datasheet.

    ab61402 has been referenced in 1 publication.

    • Ting LH  et al. Contractile forces in platelet aggregates under microfluidic shear gradients reflect platelet inhibition and bleeding risk. Nat Commun 10:1204 (2019). PubMed: 30867419

    Customer reviews and Q&As

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    Question

    Customer has kindly contacted us for further information regarding the use of this product in blocking application.

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    Answer

    Thank you for contacting us regarding ourAnti-CD42b antibody [AK2],ab61402. While we have not done blocking experiments ourselves with this product, the clone [AK2] was first created in the 1980s and has a number of published references in which it was found toblock the binding of von Willebrand Factor (VWF) to platelets as including in: WardCM,AndrewsRK, SmithAI, BerndtMC. Mocarhagin, a novel cobra venom metalloproteinase, cleaves the platelet von Willebrand factor receptor glycoprotein Ibalpha: identification of the sulfated tyrosine/anionic sequence Tyr-276-Glu-282 of glycoprotein Ibalpha as a binding site for von Willebrand factor and alpha-thrombin. Biochemistry.1996;35:4929-4938. Further papers may be found on Google Scholar:http://scholar.google.com/scholar?hl=en&q=AK2+von+willebrand&btnG=&as_sdt=1%2C38&as_sdtp= After review of some of those papers including the following I have seen thatconcentration of the antibody used in these experiments appears to be between 5-10ug/ml. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. Use our products? Submit an Abreview. Earn rewards! https://www.abcam.com/abreviews

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