Recombinant Anti-CD42b antibody [AK2] (ab61402)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [AK2] to CD42b
- Suitable for: ICC/IF, Flow Cyt, IHC-Fr
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
-
Product name
Anti-CD42b antibody [AK2]
See all CD42b primary antibodies -
Description
Mouse monoclonal [AK2] to CD42b -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, Flow Cyt, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Tissue, cells or virus corresponding to Human CD42b. Human platelets.
-
Positive control
- IHC-Fr: Human Spleen frozen tissue sections. Flow Cyt: Human whole blood and PBMCs. ICC/IF: HEL and Mouse splenocyte cells.
-
General notes
This product has switched from a hybridoma to recombinant production method on 08th March 2021.
Clone AK2 has been reported to block the binding of von Willebrand Factor (VWF) to platelets.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Primary antibody notes
Clone AK2 has been reported to block the binding of von Willebrand Factor (VWF) to platelets. -
Clonality
Monoclonal -
Clone number
AK2 -
Isotype
IgG1 -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab61402 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
1/100.
|
|
Flow Cyt |
Use a concentration of 10 µg/ml.
|
|
IHC-Fr |
Use a concentration of 1 µg/ml.
|
Notes |
---|
ICC/IF
1/100. |
Flow Cyt
Use a concentration of 10 µg/ml. |
IHC-Fr
Use a concentration of 1 µg/ml. |
Target
-
Function
GP-Ib, a surface membrane protein of platelets, participates in the formation of platelet plugs by binding to the A1 domain of vWF, which is already bound to the subendothelium. -
Involvement in disease
Non-arteritic anterior ischemic optic neuropathy
Bernard-Soulier syndrome
Bernard-Soulier syndrome A2, autosomal dominant
Pseudo-von Willebrand disease -
Sequence similarities
Contains 7 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain. -
Post-translational
modificationsGlycocalicin, which is approximately coextensive with the extracellular part of the molecule, is cleaved off by calpain during platelet lysis. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 2811 Human
- Entrez Gene: 14723 Mouse
- Omim: 606672 Human
- SwissProt: P07359 Human
- SwissProt: O35930 Mouse
- Unigene: 1472 Human
-
Alternative names
- Antigen CD42b alpha antibody
- Antigen CD42b-alpha antibody
- BDPLT1 antibody
see all
Images
-
Immunocytochemistry analysis of HEL (human Erythroleukemia erythroblast) labelling CD42b with ab61402 at 1/100 (6.3 µg/mL). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat anti Mouse IgG (Alexa Fluor® 488, ab150113) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. Cells were counterstained with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker 1/1000 (1 µg/mL), followed by Goat anti-Rabbit, AlexaFluor®594 ab150080 at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Confocal image showing membranous staining in HEL cells. -
Immunohistochemistry image of CD42b staining in a section of frozen normal human spleen performed on a Leica BOND™ system using the standard protocol.
The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab61402, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjµgated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions,primary antibody concentration and antibody incubation times. -
Flow cytometry staining of Human peripheral blood mononuclear cell (PBMC) with ab61402 (right) or mouse IgG isotype control (left) at 1/500 dilution, followed by Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution. Cells were stained with mouse IgG (Left) or ab61402 (Right). Then stained with anti-CD41 conjugated to APC.
Gated on viable cells.
-
Immunocytochemistry analysis of Mouse splenocytes labelling CD42b with ab61402 at 1/100 (6.3 µg/mL). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat anti Mouse IgG (Alexa Fluor® 488, ab150113) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. Cells were counterstained with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker 1/1000 (1 µg/mL), followed by Goat anti-Rabbit, AlexaFluor®594 ab150080 at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Confocal image showing membranous staining in mouse splenocytes. -
Flow cytometry staining of human whole blood with ab61402 (right) or mouse IgG1 kappa; (ab170190) isotype (left). Red blood cells of 200 µl blood were lysed, then cells were incubated for 30 min on ice in 1x PBS containing 10 µg/ml human IgG and 10 µll normal goat serum to block Fc receptors and non-specific protein-protein interaction followed by the antibody (ab61402) or mouse IgG1 kappa; (ab170190) isotype (1x106 in 100 µl; at 1 µg/ml) for 30 min on ice.
The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150117) was used at 1/2000 dilution for 30 min on ice.
The cells were simultaneously stained with CD41/CD61 APC. Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on granulocytes.
-
Negative control image. Immunohistochemistry image of CD42b staining in a section of frozen normal human cerebral cortex performed on a Leica BOND™ system using the standard protocol.
The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab61402, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjµgated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab61402 has been referenced in 1 publication.
- Ting LH et al. Contractile forces in platelet aggregates under microfluidic shear gradients reflect platelet inhibition and bleeding risk. Nat Commun 10:1204 (2019). PubMed: 30867419