Recombinant Anti-CD42b antibody [AK2] - BSA and Azide free (ab252264)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [AK2] to CD42b - BSA and Azide free
- Suitable for: ICC/IF, Flow Cyt, IHC-Fr
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-CD42b antibody [AK2] - BSA and Azide free
See all CD42b primary antibodies -
Description
Mouse monoclonal [AK2] to CD42b - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, Flow Cyt, IHC-Frmore details
Unsuitable for: IHC-P,IP or WB -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment. This information is considered to be commercially sensitive.
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Positive control
- ICC/IF: HEL cells and mouse splenocytes. Flow cyt: Human peripheral blood mononuclear cells
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General notes
ab252264 is the carrier-free version of ab61402.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
AK2 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab252264 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
1/50.
|
|
Flow Cyt |
1/490.
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|
IHC-Fr |
Use at an assay dependent concentration.
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Notes |
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ICC/IF
1/50. |
Flow Cyt
1/490. |
IHC-Fr
Use at an assay dependent concentration. |
Target
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Function
GP-Ib, a surface membrane protein of platelets, participates in the formation of platelet plugs by binding to the A1 domain of vWF, which is already bound to the subendothelium. -
Involvement in disease
Non-arteritic anterior ischemic optic neuropathy
Bernard-Soulier syndrome
Bernard-Soulier syndrome A2, autosomal dominant
Pseudo-von Willebrand disease -
Sequence similarities
Contains 7 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain. -
Post-translational
modificationsGlycocalicin, which is approximately coextensive with the extracellular part of the molecule, is cleaved off by calpain during platelet lysis. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 2811 Human
- Entrez Gene: 14723 Mouse
- Omim: 606672 Human
- SwissProt: P07359 Human
- SwissProt: O35930 Mouse
- Unigene: 1472 Human
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Alternative names
- Antigen CD42b alpha antibody
- Antigen CD42b-alpha antibody
- BDPLT1 antibody
see all
Images
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEL (human Erythroleukemia erythroblast) cells labelling CD42b with ab61402 at 1/50 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in HEL cells. ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker was used to counterstain tubulin at 1/1000 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at 1/1000 dilution (Red). The Nuclear counterstain was DAPI (Blue).
The negative controls are as follows:
-ve control 1: ab61402 at 1/100 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary secondary at 1/1000 dilution.
-ve control 2: ab179504 at 1/200 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary at 1/1000 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab61402).
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Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling CD42b with ab61402 at 1/490 (Right) compared with a Mouse IgG isotype control (Left). Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.
Cells were stained with mouse IgG (Left) or ab61402 (Right). Then stained with anti-CD41 conjugated to APC. Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab61402).
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse splenocytes cells labelling CD42b with ab61402 at 1/50 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in mouse splenocytes. ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker was used to counterstain tubulin at 1/1000 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at 1/1000 dilution (Red). The Nuclear counterstain was DAPI (Blue).
The negative controls are as follows:
-ve control 1: ab61402 at 1/100 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary secondary at 1/1000 dilution.
-ve control 2: ab179504 at 1/200 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary at 1/1000 dilution.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150113 AlexaFluor®488 Goat anti-Mouse secondary at 1:1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab61402).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab252264 has been referenced in 1 publication.
- Kumar RA et al. Kinetics of GPIbalpha-vWF-A1 tether bond under flow: effect of GPIbalpha mutations on the association and dissociation rates. Biophys J 85:4099-109 (2003). PubMed: 14645097